Next Article in Journal
Identification of the Human Papillomavirus Genotypes, According to the Human Immunodeficiency Virus Status in a Cohort of Women from Maputo, Mozambique
Previous Article in Journal
FMDV Leader Protein Interacts with the NACHT and LRR Domains of NLRP3 to Promote IL-1β Production
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 14
Issue 1
10.3390/v14010023
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Emerging SARS-CoV-2 Genotypes Show Different Replication Patterns in Human Pulmonary and Intestinal Epithelial Cells

by
Gabriel Augusto Pires de Souza
1,2,
Marion Le Bideau
1,2,
Celine Boschi
1,2,
Lorène Ferreira
1,2,
Nathalie Wurtz
1,2,
Christian Devaux
1,2,
Philippe Colson
1,2 and
Bernard La Scola
1,2,*
1
Unité de Recherche Microbe Phylogeny and Evoluition (MEPHI), Institut de Recherche pour le Développement (IRD), Assistance Publique—Hôpitaux de Marseille (AP-HM), Aix-Marseille Université, 27 Boulevard Jean Moulin, 13005 Marseille, France
2
IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, France
*
Author to whom correspondence should be addressed.
Viruses 2022, 14(1), 23; https://doi.org/10.3390/v14010023
Submission received: 22 September 2021 / Revised: 29 November 2021 / Accepted: 21 December 2021 / Published: 23 December 2021
(This article belongs to the Topic Burden of COVID-19 in Different Countries)
Browse Figures
Versions Notes

Abstract

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) quickly spread worldwide following its emergence in Wuhan, China, and hit pandemic levels. Its tremendous incidence favoured the emergence of viral variants. The current genome diversity of SARS-CoV-2 has a clear impact on epidemiology and clinical practice, especially regarding transmission rates and the effectiveness of vaccines. In this study, we evaluated the replication of different SARS-CoV-2 isolates representing different virus genotypes which have been isolated throughout the pandemic. We used three distinct cell lines, including Vero E6 cells originating from monkeys; Caco-2 cells, an intestinal epithelium cell line originating from humans; and Calu-3 cells, a pulmonary epithelium cell line also originating from humans. We used RT-qPCR to replicate different SARS-CoV-2 genotypes by quantifying the virus released in the culture supernatant of infected cells. We found that the different viral isolates replicate similarly in Caco-2 cells, but show very different replicative capacities in Calu-3 cells. This was especially highlighted for the lineages B.1.1.7, B.1.351 and P.1, which are considered to be variants of concern. These results underscore the importance of the evaluation and characterisation of each SARS-CoV-2 isolate in order to establish the replication patterns before performing tests, and of the consideration of the ideal SARS-CoV-2 genotype–cell type pair for each assay.
Keywords: SARS-CoV-2; COVID-19; viral culture; isolate; genotype; variants; Vero E6 cells; Caco-2 cells; Calu-3 cells SARS-CoV-2; COVID-19; viral culture; isolate; genotype; variants; Vero E6 cells; Caco-2 cells; Calu-3 cells

Share and Cite

MDPI and ACS Style

de Souza, G.A.P.; Le Bideau, M.; Boschi, C.; Ferreira, L.; Wurtz, N.; Devaux, C.; Colson, P.; La Scola, B. Emerging SARS-CoV-2 Genotypes Show Different Replication Patterns in Human Pulmonary and Intestinal Epithelial Cells. Viruses 2022, 14, 23. https://doi.org/10.3390/v14010023

AMA Style

de Souza GAP, Le Bideau M, Boschi C, Ferreira L, Wurtz N, Devaux C, Colson P, La Scola B. Emerging SARS-CoV-2 Genotypes Show Different Replication Patterns in Human Pulmonary and Intestinal Epithelial Cells. Viruses. 2022; 14(1):23. https://doi.org/10.3390/v14010023

Chicago/Turabian Style

de Souza, Gabriel Augusto Pires, Marion Le Bideau, Celine Boschi, Lorène Ferreira, Nathalie Wurtz, Christian Devaux, Philippe Colson, and Bernard La Scola. 2022. "Emerging SARS-CoV-2 Genotypes Show Different Replication Patterns in Human Pulmonary and Intestinal Epithelial Cells" Viruses 14, no. 1: 23. https://doi.org/10.3390/v14010023

APA Style

de Souza, G. A. P., Le Bideau, M., Boschi, C., Ferreira, L., Wurtz, N., Devaux, C., Colson, P., & La Scola, B. (2022). Emerging SARS-CoV-2 Genotypes Show Different Replication Patterns in Human Pulmonary and Intestinal Epithelial Cells. Viruses, 14(1), 23. https://doi.org/10.3390/v14010023

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop