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Volume 13
Issue 8
10.3390/v13081428
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Article

Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter

by
Daniela D’Angelantonio
1,†,
Silvia Scattolini
1,†,
Arianna Boni
2,
Diana Neri
3,
Gabriella Di Serafino
4,
Philippa Connerton
5,
Ian Connerton
5,
Francesco Pomilio
1,
Elisabetta Di Giannatale
1,
Giacomo Migliorati
1 and
Giuseppe Aprea
1,*
1
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, Italy
2
Istituto Superiore di Sanità, 00161 Rome, Italy
3
Local Health Unit of Ferrara (USL Ferrara), 44121 Ferrara, Italy
4
Local Health Unit of Lanciano-vasto-Chieti (ASL Chieti), 66100 Chieti, Italy
5
Division of Food Science, School of Bioscience, The University of Nottingham, Nottingham LE12 5RD, UK
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editors: Mansel Griffiths and Hany Anany
Viruses 2021, 13(8), 1428; https://doi.org/10.3390/v13081428
Received: 7 July 2021 / Revised: 16 July 2021 / Accepted: 19 July 2021 / Published: 22 July 2021
(This article belongs to the Special Issue Bacteriophages in Food Applications)
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Abstract

Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni is the main cause of the infection, and bacterial colonization in broiler chickens is widespread and difficult to prevent, leading to high risk of occurrence in broiler meat. Phage therapy represents an alternative strategy to control Campylobacter in poultry. The aim of this work was to assess the efficacy of two field-isolated bacteriophages against experimental infections with an anti-microbial resistant (AMR) Campylobacter jejuni strain. A two-step phage application was tested according to a specific combination between chickens’ rearing time and specific multiplicities of infections (MOIs), in order to reduce the Campylobacter load in the animals at slaughtering and to limit the development of phage-resistant mutants. In particular, 75 broilers were divided into three groups (A, B and C), and phages were administered to animals of groups B and C at day 38 (Φ 16-izsam) and 39 (Φ 7-izsam) at MOI 0.1 (group B) and 1 (group C). All broilers were euthanized at day 40, and Campylobacter jejuni was enumerated in cecal contents. Reductions in Campylobacter counts were statistically significant in both group B (1 log10 colony forming units (cfu)/gram (gr)) and group C (2 log10 cfu/gr), compared to the control group. Our findings provide evidence about the ability of phage therapy to reduce the Campylobacter load in poultry before slaughtering, also associated with anti-microbial resistance pattern. View Full-Text
Keywords: anti-microbial resistance (AMR); broiler chickens; Campylobacter jejuni; phage therapy anti-microbial resistance (AMR); broiler chickens; Campylobacter jejuni; phage therapy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style

D’Angelantonio, D.; Scattolini, S.; Boni, A.; Neri, D.; Di Serafino, G.; Connerton, P.; Connerton, I.; Pomilio, F.; Di Giannatale, E.; Migliorati, G.; Aprea, G. Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter. Viruses 2021, 13, 1428. https://doi.org/10.3390/v13081428

AMA Style

D’Angelantonio D, Scattolini S, Boni A, Neri D, Di Serafino G, Connerton P, Connerton I, Pomilio F, Di Giannatale E, Migliorati G, Aprea G. Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter. Viruses. 2021; 13(8):1428. https://doi.org/10.3390/v13081428

Chicago/Turabian Style

D’Angelantonio, Daniela, Silvia Scattolini, Arianna Boni, Diana Neri, Gabriella Di Serafino, Philippa Connerton, Ian Connerton, Francesco Pomilio, Elisabetta Di Giannatale, Giacomo Migliorati, and Giuseppe Aprea. 2021. "Bacteriophage Therapy to Reduce Colonization of Campylobacter jejuni in Broiler Chickens before Slaughter" Viruses 13, no. 8: 1428. https://doi.org/10.3390/v13081428

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