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LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses

Laboratory for Genome Engineering and Synthetic Biology, King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Saudi Arabia
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Eugene Savenkov and Katalin Nemes
Viruses 2021, 13(3), 466; https://doi.org/10.3390/v13030466
Received: 13 January 2021 / Revised: 21 February 2021 / Accepted: 26 February 2021 / Published: 12 March 2021
(This article belongs to the Special Issue Molecular Plant-Virus Interactions)
One important factor for successful disease management is the ability to rapidly and accurately identify the causal agent. Plant viruses cause severe economic losses and pose a serious threat to sustainable agriculture. Therefore, optimization of the speed, sensitivity, feasibility, portability, and accuracy of virus detection is urgently needed. Here, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid diagnostic method utilizing the CRISPR–Cas12a system for detecting two geminiviruses, tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV), which have single-stranded DNA genomes. Our assay detected TYLCV and ToLCNDV in infected plants with high sensitivity and specificity. Our newly developed assay can be performed in ~1 h and provides easy-to-interpret visual readouts using a simple, low-cost fluorescence visualizer, making it suitable for point-of-use applications. View Full-Text
Keywords: molecular diagnostics; plant viruses; CRISPR–Cas12; biosensing; genome engineering molecular diagnostics; plant viruses; CRISPR–Cas12; biosensing; genome engineering
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MDPI and ACS Style

Mahas, A.; Hassan, N.; Aman, R.; Marsic, T.; Wang, Q.; Ali, Z.; Mahfouz, M.M. LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses. Viruses 2021, 13, 466. https://doi.org/10.3390/v13030466

AMA Style

Mahas A, Hassan N, Aman R, Marsic T, Wang Q, Ali Z, Mahfouz MM. LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses. Viruses. 2021; 13(3):466. https://doi.org/10.3390/v13030466

Chicago/Turabian Style

Mahas, Ahmed, Norhan Hassan, Rashid Aman, Tin Marsic, Qiaochu Wang, Zahir Ali, and Magdy M. Mahfouz 2021. "LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses" Viruses 13, no. 3: 466. https://doi.org/10.3390/v13030466

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