BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Cohort
2.2. Nucleic Acid Extraction
2.3. Real-Time PCR Assay for BKPyV DNA
2.4. miRNA RT-PCR Assay
2.5. Statistical Analyses
3. Results
3.1. Case-Control Analysis
3.2. Urine BKPyV miRNA Levels Are Less Accurate than DNA Levels as Marker of DNAemia
3.3. BKPyV miRNAs: Changes over Time Following Kidney Transplantation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cases | p Value | ||||
---|---|---|---|---|---|
Patients | Controls | BKU | BKP | Controls vs. BKU | Controls vs. BKP |
n (total = 43) | 24 | 19 | 17 | ||
Age (mean ± SEM) | 52.29 ± 2.66 | 56.05 ± 3.30 | 58.00 ± 3.28 | 0.375 | 0.182 |
Male:female sex ratio | 1.4 | 2.17 | 2.4 | 0.542 | 0.52 |
Induction therapy | |||||
Basiliximab (vs. ATG) | 16 (vs. 8) | 9 (vs. 10) | 8 (vs. 9) | 0.23 | 0.335 |
Maintenance therapy | |||||
Tacrolimus (vs. Cyclosporine) | 21 (vs. 3) | 19 (vs. 0) | 17 (vs. 0) | 0.242 | 0.254 |
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Demey, B.; Descamps, V.; Presne, C.; Helle, F.; Francois, C.; Duverlie, G.; Castelain, S.; Brochot, E. BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients. Viruses 2021, 13, 351. https://doi.org/10.3390/v13020351
Demey B, Descamps V, Presne C, Helle F, Francois C, Duverlie G, Castelain S, Brochot E. BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients. Viruses. 2021; 13(2):351. https://doi.org/10.3390/v13020351
Chicago/Turabian StyleDemey, Baptiste, Véronique Descamps, Claire Presne, Francois Helle, Catherine Francois, Gilles Duverlie, Sandrine Castelain, and Etienne Brochot. 2021. "BK Polyomavirus Micro-RNAs: Time Course and Clinical Relevance in Kidney Transplant Recipients" Viruses 13, no. 2: 351. https://doi.org/10.3390/v13020351