Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacteria, Media, and Growth Conditions
2.2. Propagation of Phage vB_EfaS-271
2.3. Phage Titration
2.4. Determination of Number of Colony Forming Units
2.5. Formation and Assessment of Biofilm Formed on Catheters
2.6. Assessment of vB_EfaS-271 Phage Cytotoxicity
2.7. Microscopic Analyses
2.8. Assessment of Appearance of Phage-Resistant Bacteria
2.9. Statistical Analysis
3. Results
3.1. Efficiency of Phage Treatment of Biofilms Formed on Catheters
3.2. Assessment of Toxicity of vB_EfaS-271 Phage Particles to Mammalian Cells and Effects of This Phage on E. faecalis Co-Cultured with These Cells
3.3. Appearance of Phage-Resistant Bacteria
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Topka-Bielecka, G.; Nejman-Faleńczyk, B.; Bloch, S.; Dydecka, A.; Necel, A.; Węgrzyn, A.; Węgrzyn, G. Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis. Viruses 2021, 13, 318. https://doi.org/10.3390/v13020318
Topka-Bielecka G, Nejman-Faleńczyk B, Bloch S, Dydecka A, Necel A, Węgrzyn A, Węgrzyn G. Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis. Viruses. 2021; 13(2):318. https://doi.org/10.3390/v13020318
Chicago/Turabian StyleTopka-Bielecka, Gracja, Bożena Nejman-Faleńczyk, Sylwia Bloch, Aleksandra Dydecka, Agnieszka Necel, Alicja Węgrzyn, and Grzegorz Węgrzyn. 2021. "Phage–Bacteria Interactions in Potential Applications of Bacteriophage vB_EfaS-271 against Enterococcus faecalis" Viruses 13, no. 2: 318. https://doi.org/10.3390/v13020318