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Article

Circulation of Fluorescently Labelled Phage in a Murine Model

1
Research and Development Center, Regional Specialist Hospital, Kamieńskiego 73a, 51-154 Wroclaw, Poland
2
Bacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland
3
Laboratory of Experimental Anticancer Therapy, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Zuzanna Drulis-Kawa and Daria Augustyniak
Viruses 2021, 13(2), 297; https://doi.org/10.3390/v13020297
Received: 21 January 2021 / Revised: 10 February 2021 / Accepted: 11 February 2021 / Published: 14 February 2021
(This article belongs to the Special Issue Phage-Bacteria Interplay in Health and Disease)
Interactions between bacteriophages and mammals strongly affect possible applications of bacteriophages. This has created a need for tools that facilitate studies of phage circulation and deposition in tissues. Here, we propose red fluorescent protein (RFP)-labelled E. coli lytic phages as a new tool for the investigation of phage interactions with cells and tissues. The interaction of RFP-labelled phages with living eukaryotic cells (macrophages) was visualized after 20 min of co-incubation. RFP-labeled phages were applied in a murine model of phage circulation in vivo. Phages administered by three different routes (intravenously, orally, rectally) were detected through the course of time. The intravenous route of administration was the most efficient for phage delivery to multiple body compartments: 20 min after administration, virions were detected in lymph nodes, lungs, and liver; 30 min after administration, they were detectable in muscles; and 1 h after administration, phages were detected in spleen and lymph nodes. Oral and rectal administration of RFP-labelled phages allowed for their detection in the gastrointestinal (GI) tract only. View Full-Text
Keywords: molecular imaging; phage circulation; RFP; HAP phage; pharmacokinetics; murine model molecular imaging; phage circulation; RFP; HAP phage; pharmacokinetics; murine model
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MDPI and ACS Style

Kaźmierczak, Z.; Majewska, J.; Milczarek, M.; Owczarek, B.; Dąbrowska, K. Circulation of Fluorescently Labelled Phage in a Murine Model. Viruses 2021, 13, 297. https://doi.org/10.3390/v13020297

AMA Style

Kaźmierczak Z, Majewska J, Milczarek M, Owczarek B, Dąbrowska K. Circulation of Fluorescently Labelled Phage in a Murine Model. Viruses. 2021; 13(2):297. https://doi.org/10.3390/v13020297

Chicago/Turabian Style

Kaźmierczak, Zuzanna, Joanna Majewska, Magdalena Milczarek, Barbara Owczarek, and Krystyna Dąbrowska. 2021. "Circulation of Fluorescently Labelled Phage in a Murine Model" Viruses 13, no. 2: 297. https://doi.org/10.3390/v13020297

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