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Article

Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana

1
West African Center for Cell Biology of Infectious Pathogens (WACCBIP), Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Accra 00233, Ghana
2
Ear, Nose and Throat Unit, Korle-Bu Teaching Hospital, Accra 00233, Ghana
3
School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(7), 766; https://doi.org/10.3390/v12070766
Received: 1 May 2020 / Revised: 5 June 2020 / Accepted: 8 June 2020 / Published: 16 July 2020
Nasopharyngeal cancer (NPC) is associated with Epstein Barr virus (EBV) infection. However different viral strains have been implicated in NPC worldwide. This study aimed to detect and characterize EBV in patients diagnosed with NPC in Ghana. A total of 55 patients diagnosed with NPC by CT scan and endoscopy were age-matched with 53 controls without a known oncological disease. Venous blood was collected from the study participants and DNA extracted from the blood samples. Detection of EBV and genotyping were done by amplifying Epstein Barr nuclear antigen 1 (EBNA-1) and Epstein Barr nuclear antigen 2 (EBNA-2), respectively, using specific primers. Viral load in patients and controls was determined using real-time polymerase chain reaction. EBV positivity in controls (92%) was significantly greater than that of NPC patients (67%) (χ2 = 19.17, p < 0.0001), and viral infection was independent of gender (χ2 = 1.770, p = 0.1834). The predominant EBV genotypes in patients and controls were genotype 2 (52%) and genotype 1 (62%), respectively. Median EBV load was significantly higher in NPC patients than the control group (p < 0.01). In summary, prevalence of EBV genotype 2 infection was higher in NPC patients than the control group. Assessment of EBV load may be used as a biomarker for the diagnosis of NPC. View Full-Text
Keywords: nasopharyngeal cancer; Epstein Barr virus; genotypes; viral load; Ghana nasopharyngeal cancer; Epstein Barr virus; genotypes; viral load; Ghana
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MDPI and ACS Style

Ayee, R.; Ofori, M.E.O.; Tagoe, E.A.; Languon, S.; Searyoh, K.; Armooh, L.; Bilson-Amoah, E.; Baidoo, K.; Kitcher, E.; Wright, E.; Quaye, O. Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana. Viruses 2020, 12, 766. https://doi.org/10.3390/v12070766

AMA Style

Ayee R, Ofori MEO, Tagoe EA, Languon S, Searyoh K, Armooh L, Bilson-Amoah E, Baidoo K, Kitcher E, Wright E, Quaye O. Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana. Viruses. 2020; 12(7):766. https://doi.org/10.3390/v12070766

Chicago/Turabian Style

Ayee, Richmond; Ofori, Maame E.O.; Tagoe, Emmanuel A.; Languon, Sylvester; Searyoh, Kafui; Armooh, Louis; Bilson-Amoah, Estella; Baidoo, Kenneth; Kitcher, Emmanuel; Wright, Edward; Quaye, Osbourne. 2020. "Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana" Viruses 12, no. 7: 766. https://doi.org/10.3390/v12070766

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