Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimens
2.2. Serology
2.3. Virus and Nucleic Acid Detection
2.4. Immunohistochemistry (IHC)
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | VNT (Titre) |
---|---|---|---|---|---|---|
28/08/2012 | Serum | 21 | Negative | 512 | ||
22/10/2012 | Serum | 23 | Negative | ≥512 | ||
12/11/2012 | Extended semen | 23 | Positive (35.4) | Negative | ||
23/11/2012 | Extended semen | 24 | Positive (35.4) | Negative | ||
26/11/2012 | Extended semen | 24 | Positive (35.8) | Negative | ||
28/11/2012 | Extended semen | 24 | Positive (32.8) | Negative | ||
30/11/2012 | Extended semen | 24 | Positive (35.5) | Negative | ||
6/12/2012 | Serum | 24 | ≥512 |
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | AGID | VNT (Titre) |
---|---|---|---|---|---|---|---|
23/01/2012 | Serum | 10 | Negative | 3 | |||
13/03/2012 | Serum | 12 | Negative | 2 | |||
17/04/2012 | Extended semen | 13 | Negative a | Negative | |||
17/04/2012 | Extended semen | 15 | Negative a | ||||
19/06/2012 | Extended semen | 15 | Negative a | ||||
29/06/2012 | Extended semen | 16 | Negative a | ||||
20/07/2012 | Extended semen | 17 | Negative a | ||||
8/08/2012 | Extended semen | 17 | Negative a | ||||
20/08/2012 | Extended semen | 17 | Negative a | ||||
24/08/2012 | Extended semen | 17 | Negative a | ||||
31/08/2012 | Extended semen | 18 | Negative a | ||||
4/09/2012 | Extended semen | 18 | Negative a | ||||
7/09/2012 | Extended semen | 18 | Negative a | ||||
11/09/2012 | Extended semen | 18 | Negative a | ||||
21/09/2012 | Extended semen | 18 | Negative a | ||||
28/09/2012 | Extended semen | 19 | Negative a | ||||
26/10/2012 | Extended semen | 20 | Positive (36.5) a | ||||
13/11/2012 | Serum | 26 | Negative | >3 | |||
7/05/2013 | Serum | 38 | Negative | 3 | |||
5/05/2014 | Serum | 50 | Negative | 3 | |||
4/05/2015 | Serum | 62 | Negative | 3 | |||
2/05/2016 | Serum | 74 | Negative | ≥512 | |||
1/05/2017 | Extended semen | 82 | Positive (29.7) | Positive | |||
18/01/2018 | Extended semen | 82 | Positive (32.6) | Negative | |||
23/01/2018 | Extended semen | 82 | Positive (32.4) | Negative | |||
25/01/2018 | Extended semen | 83 | Positive (30.9) | Positive | |||
1/02/2018 | Extended semen | 83 | Positive (35.9) | Negative | |||
6/02/2018 | Extended semen | 83 | Positive (32.5) | Negative | |||
8/02/2018 | Serum | 83 | Negative | ||||
12/02/2018 | Extended semen | 83 | Positive (33.3) | Negative | |||
13/02/2018 | Extended semen | 83 | Positive (34.4) | Negative | |||
15/02/2018 | Extended semen | 83 | Positive (31.58) | Negative | |||
16/02/2018 | Extended semen | 83 | Positive (38.46) | ||||
20/02/2018 | Extended semen | 83 | Positive (36.82) | ||||
22/02/2018 | Extended semen | 83 | Negative | ||||
27/02/2018 | Extended semen | 84 | Negative |
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | VNT (Titre) |
---|---|---|---|---|---|---|
20/02/2013 | Serum | 15 | Negative | |||
22/05/2013 | Extended semen | 18 | Positive (29.2) | |||
24/05/2013 | Extended semen | 18 | Positive (29.7) | Negative | ||
28/05/2013 | Extended semen | 18 | Positive (29.3) | Negative | ||
30/05/2013 | Extended semen | 18 | Positive (29.1) | |||
3/06/2013 | Extended semen | 18 | Positive (32.6) | |||
7/06/2013 | Extended semen | 18 | Positive (30.0) | |||
10/06/2013 | Serum | 18 | Negative | ≥512 | ||
12/06/2013 | Extended semen | 18 | Positive (31.0) | |||
14/06/2013 | Extended semen | 19 | Positive (30.5) | |||
18/06/2013 | Extended semen | 19 | Positive (29.5) | |||
20/06/2013 | Extended semen | 19 | Positive (30.0) | |||
24/06/2013 | Extended semen | 19 | Positive (30.4) | |||
26/06/2013 | Extended semen | 19 | Positive (30.5) | |||
3/07/2013 | Extended semen | 19 | Positive (30.5) | |||
8/07/2013 | Extended semen | 19 | Positive (32.0) | |||
12/07/2013 | Extended semen | 19 | Positive (31.0) | |||
12/07/2013 | Extended semen | 19 | Positive (30.5) | Negative | ||
16/07/2013 | Extended semen | 20 | Positive (30.4) | |||
18/07/2013 | Extended semen | 20 | Positive (31.4) | |||
23/07/2013 | Extended semen | 20 | Positive (32.2) | |||
15/08/2013 | Extended semen | 21 | Positive (30.7) | Negative |
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | AGID | VNT (Titre) |
---|---|---|---|---|---|---|---|
3/09/2013 | Serum | 18 | Negative | Negative | |||
8/10/2013 | Serum | 19 | Negative | >3 | |||
22/10/2013 | Extended semen | 20 | Positive (31.7) | Positive | |||
24/10/2013 | Extended semen | 20 | Positive (32.0) | Negative | |||
16/12/2013 | Seminal fluid | 22 | Positive (30.2) | Negative | |||
16/02/2014 a | Extended semen | 24 | Positive (30.1) | ||||
16/02/2014 a | Serum | 24 | ≥512 |
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | VNT (Titre) |
---|---|---|---|---|---|---|
31/03/2014 | Serum | 20 | Negative | 4 | ||
12/05/2014 | Serum | 21 | Negative | 256 | ||
15/05/2014 | Extended semen | 21 | Positive (32.6) | |||
24/05/2014 | Neat semen | 22 | Positive (34.2) | Negative | ||
11/06/2014 | Extended semen | 22 | Positive (34.8) | Negative | ||
2/07/2014 | Neat semen | 23 | Positive (34.1) | Negative | ||
17/07/2014 | Extended semen | 23 | Positive (33.9) | Negative | ||
20/02/2015 | Serum | 31 | Negative | ≥512 | ||
8/04/2015 | Extended semen | 32 | Negative | |||
20/04/2015 | Serum | 32 | Negative | ≥512 | ||
22/04/2015 | Extended semen | 33 | Negative | |||
27/04/2015 | Extended semen | 33 | Negative | |||
14/05/2015 | Extended semen | 33 | Negative | |||
19/05/2015 | Extended semen | 33 | Negative | |||
21/05/2015 | Extended semen | 34 | Negative | |||
25/05/2015 | Extended semen | 34 | Negative | |||
28/05/2015 | Extended semen | 34 | Negative | |||
2/06/2015 | Extended semen | 34 | Negative | |||
4/06/2015 | Extended semen | 34 | Negative | |||
8/06/2015 | Extended semen | 34 | Negative | |||
12/06/2015 | Extended semen | 34 | Negative | |||
16/06/2015 | Extended semen | 34 | Negative | |||
28/06/2015 | Extended semen | 35 | Negative | |||
18/07/2015 | Extended semen | 35 | Negative | |||
17/08/2015 | Extended semen | 36 | Negative | |||
21/08/2015 | Extended semen | 37 | Negative | |||
24/08/2015 | Extended semen | 37 | Negative | |||
31/08/2015 | Extended semen | 38 | Negative | |||
6/02/2017 | Serum | 54 | Negative | 512 | ||
3/04/2017 | Serum | 56 | 128 | |||
1/05/2017 | Extended semen | 57 | Negative | |||
4/05/2017 | Extended semen | 57 | Negative |
Sample Date | Sample Type | Age (Months) | qRT-PCR (Ct) | Virus Isolation | Antigen ELISA | VNT |
---|---|---|---|---|---|---|
14/12/2018 | Extended semen | 15 | Negative | |||
6/04/2019 a | Serum | 19 | Negative | |||
21/05/2019 | Extended semen | 20 | Positive (27.6) | Positive | ||
1/09/2019 | Serum | 24 | Negative | >512 | ||
24/09/2019 | Serum | 24 | >512 | |||
17/10/2019 | Extended semen | 25 | Positive (38.0) | Negative | ||
22/11/2019 | Extended semen | 26 | Positive (36.4) | |||
8/01/2020 | Extended semen | 28 | Positive (36.2) | |||
23/01/2020 | Extended semen | 28 | Negative |
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Bull Number | Breed | Estimated Age at First Detection of Infection (Months) a | Estimated Duration of Shedding (Months) b | qRT-PCR Results in Semen (Ct) | Virus Isolation (Pos/Total Tested) |
---|---|---|---|---|---|
1 | Main Anjou | 21 | 3 | 32.8–35.8 | 0/6 |
2 | Holstein | 10 | 73 | 29.7–38.4 | 2/10 |
3 | Angus | 18 | 3 | 29.1–32.6 | 0/5 |
4 | Angus | 19 | 4 | 30.1–32.0 | 1/3 |
5 | Angus | 20 | 6 | 32.6–34.8 | 0/6 |
6 | Wagyu | 21 | 8 | 27.6–38.0 | 1/2 c |
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Read, A.J.; Gestier, S.; Parrish, K.; Finlaison, D.S.; Gu, X.; O’Connor, T.W.; Kirkland, P.D. Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls. Viruses 2020, 12, 674. https://doi.org/10.3390/v12060674
Read AJ, Gestier S, Parrish K, Finlaison DS, Gu X, O’Connor TW, Kirkland PD. Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls. Viruses. 2020; 12(6):674. https://doi.org/10.3390/v12060674
Chicago/Turabian StyleRead, Andrew J., Sarah Gestier, Kate Parrish, Deborah S. Finlaison, Xingnian Gu, Tiffany W. O’Connor, and Peter D. Kirkland. 2020. "Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls" Viruses 12, no. 6: 674. https://doi.org/10.3390/v12060674
APA StyleRead, A. J., Gestier, S., Parrish, K., Finlaison, D. S., Gu, X., O’Connor, T. W., & Kirkland, P. D. (2020). Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls. Viruses, 12(6), 674. https://doi.org/10.3390/v12060674