Next Article in Journal
Genomic Characterization, Formulation and Efficacy in Planta of a Siphoviridae and Podoviridae Protection Cocktail against the Bacterial Plant Pathogens Pectobacterium spp.
Next Article in Special Issue
Reduce and Control: A Combinatorial Strategy for Achieving Sustained HIV Remissions in the Absence of Antiretroviral Therapy
Previous Article in Journal
Manipulation of Jasmonate Signaling by Plant Viruses and Their Insect Vectors
Previous Article in Special Issue
The Impact of Cellular Proliferation on the HIV-1 Reservoir
Open AccessReview

Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence

1
HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University, 9000 Ghent, Belgium
2
BioBix, Department of Data Analysis and Mathematical Modelling, Faculty of Bioscience Engineering, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(2), 149; https://doi.org/10.3390/v12020149
Received: 20 December 2019 / Revised: 24 January 2020 / Accepted: 26 January 2020 / Published: 28 January 2020
(This article belongs to the Special Issue HIV-1 Latency: Regulation and Reversal)
While current antiretroviral therapies are able to halt HIV-1 progression, they are not curative, as an interruption of treatment usually leads to viral rebound. The persistence of this stable HIV-1 latent reservoir forms the major barrier in HIV-1 cure research. The need for a better understanding of the mechanisms behind reservoir persistence resulted in the development of several novel assays allowing to perform an extensive in-depth characterization. The objective of this review is to present an overview of the current state-of-the-art PCR-based technologies to study the replication-competent HIV-1 reservoir. Here, we outline the advantages, limitations, and clinical relevance of different approaches. Future HIV-1 eradication studies would benefit from information-rich, high-throughput assays as they provide a more efficient and standardized way of characterizing the persisting HIV-1 reservoir. View Full-Text
Keywords: HIV-1; HIV-1 reservoir; PCR; sequencing; NGS; replication-competent; integration site; HIV-1 genome HIV-1; HIV-1 reservoir; PCR; sequencing; NGS; replication-competent; integration site; HIV-1 genome
Show Figures

Figure 1

MDPI and ACS Style

Lambrechts, L.; Cole, B.; Rutsaert, S.; Trypsteen, W.; Vandekerckhove, L. Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence. Viruses 2020, 12, 149. https://doi.org/10.3390/v12020149

AMA Style

Lambrechts L, Cole B, Rutsaert S, Trypsteen W, Vandekerckhove L. Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence. Viruses. 2020; 12(2):149. https://doi.org/10.3390/v12020149

Chicago/Turabian Style

Lambrechts, Laurens; Cole, Basiel; Rutsaert, Sofie; Trypsteen, Wim; Vandekerckhove, Linos. 2020. "Emerging PCR-Based Techniques to Study HIV-1 Reservoir Persistence" Viruses 12, no. 2: 149. https://doi.org/10.3390/v12020149

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop