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Dengue: A Minireview
Article

Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment

1
Imunologia Celular e Molecular, Instituto René Rachou, Belo Horizonte, MG 30190-002, Brazil
2
Helmintologia e Malacologia Médica, Instituto René Rachou, Belo Horizonte, MG 30190-002, Brazil
3
Secretaria Municipal de Saúde, Prefeitura de Belo Horizonte, Belo Horizonte, MG 30550-340, Brazil
4
Informática de Biossistemas, Instituto René Rachou, Belo Horizonte, MG 30190-002, Brazil
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(11), 1284; https://doi.org/10.3390/v12111284
Received: 7 October 2020 / Revised: 29 October 2020 / Accepted: 30 October 2020 / Published: 10 November 2020
(This article belongs to the Special Issue 7th Pan-American Dengue Research Network Meeting: Lima 2020)
Dengue is an acute viral disease caused by Dengue virus (DENV) and is considered to be the most common arbovirus worldwide. The clinical characteristics of dengue may vary from asymptomatic to severe complications and severe organ impairment, particularly affecting the liver. Dengue treatment is palliative with acetaminophen (APAP), usually known as Paracetamol, being the most used drug aiming to relieve the mild symptoms of dengue. APAP is a safe and effective drug but, like dengue, can trigger the development of liver disorders. Given this scenario, it is necessary to investigate the effects of combining these two factors on hepatocyte homeostasis. Therefore, this study aimed to evaluate the molecular changes in hepatocytes resulting from the association between DENV infection and treatment with sub-toxic APAP concentrations. Using an in vitro experimental model of DENV-2 infected hepatocytes (AML-12 cells) treated with APAP, we evaluated the influence of the virus and drug association on the transcriptome of these hepatocytes by RNA sequencing (RNAseq). The virus–drug association was able to induce changes in the gene expression profile of AML-12 cells and here we highlight and explore these changes and its putative influence on biological processes for cellular homeostasis. View Full-Text
Keywords: Dengue; DENV; acetaminophen; hepatocytes; transcriptome; RNA sequencing (RNAseq) Dengue; DENV; acetaminophen; hepatocytes; transcriptome; RNA sequencing (RNAseq)
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MDPI and ACS Style

Ferreira, J.G.G.; Gava, S.G.; Oliveira, E.S.; Batista, I.C.A.; Fernandes, G.d.R.; Mourão, M.M.; Calzavara-Silva, C.E. Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment. Viruses 2020, 12, 1284. https://doi.org/10.3390/v12111284

AMA Style

Ferreira JGG, Gava SG, Oliveira ES, Batista ICA, Fernandes GdR, Mourão MM, Calzavara-Silva CE. Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment. Viruses. 2020; 12(11):1284. https://doi.org/10.3390/v12111284

Chicago/Turabian Style

Ferreira, Jorge G.G., Sandra G. Gava, Eneida S. Oliveira, Izabella C.A. Batista, Gabriel d.R. Fernandes, Marina M. Mourão, and Carlos E. Calzavara-Silva 2020. "Gene Expression Signatures in AML-12 Hepatocyte Cells upon Dengue virus Infection and Acetaminophen Treatment" Viruses 12, no. 11: 1284. https://doi.org/10.3390/v12111284

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