Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Lines
2.3. Stimulation of THP-1 Cells and Differentiation into Adherent Macrophages
2.4. Plasmid Construction
2.5. Lentivirus Construction
2.6. Real-Time PCR
2.7. Western Blotting
2.8. 50% Tissue Culture Infective Dose (TCID50) Assay
2.9. Plaque Assay
2.10. Coimmunoprecipitation (Co-IP)
2.11. Confocal Microscopy
2.12. Oligonucleotide Microarray Assay
2.13. Statistical Analyses
3. Results
3.1. ILF2 Represses EV71 Infection in RD Cells
3.2. ILF2 Expression is Attenuated in EV71-Infected Cells
3.3. EV71 Represses ILF2 mRNA Expression and Protein Production
3.4. EV71 2B Interacts and Colocalizes with ILF2 in the Cytoplasm
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Jin, J.; Wang, W.; Ai, S.; Liu, W.; Song, Y.; Luo, Z.; Zhang, Q.; Wu, K.; Liu, Y.; Wu, J. Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects. Viruses 2020, 12, 22. https://doi.org/10.3390/v12010022
Jin J, Wang W, Ai S, Liu W, Song Y, Luo Z, Zhang Q, Wu K, Liu Y, Wu J. Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects. Viruses. 2020; 12(1):22. https://doi.org/10.3390/v12010022
Chicago/Turabian StyleJin, Jing, Wenbiao Wang, Sha Ai, Weiyong Liu, Yu Song, Zhen Luo, Qi Zhang, Kailang Wu, Yingle Liu, and Jianguo Wu. 2020. "Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects" Viruses 12, no. 1: 22. https://doi.org/10.3390/v12010022