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Open AccessArticle

Detection of Two Highly Diverse Peribunyaviruses in Mosquitoes from Palenque, Mexico

1
Berlin Institute of Health, Institute of Virology, Charité—Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 10117 Berlin, Germany
2
Institute of Virology, University of Bonn Medical Centre, Bonn, Germany, Biotest AG, 63303 Dreieich, Germany
3
Department of Biology, Universität Regensburg, 93053 Regensburg, Germany
4
Estación de Biología Tropical Los Tuxtlas, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City 04513, Mexico
5
ProBioGen AG, 13086 Berlin, Germany
6
Department of Environmental Sciences and Program in Population Biology, Ecology and Evolution, Emory University, Atlanta, GA 30322, USA
7
Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA 30322, USA
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(9), 832; https://doi.org/10.3390/v11090832
Received: 16 August 2019 / Revised: 5 September 2019 / Accepted: 6 September 2019 / Published: 7 September 2019
(This article belongs to the Special Issue Emerging Arboviruses)
The Peribunyaviridae family contains the genera Orthobunyavirus, Herbevirus, Pacuvirus, and Shangavirus. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insects. Here, we tested mosquitoes from a tropical rainforest in Mexico for infections with peribunyaviruses. We identified and characterized two previously unknown viruses, designated Baakal virus (BKAV) and Lakamha virus (LAKV). Sequencing and de novo assembly of the entire BKAV and LAKV genomes revealed that BKAV is an orthobunyavirus and LAKV is likely to belong to a new genus. LAKV was almost equidistant to the established peribunyavirus genera and branched as a deep rooting solitary lineage basal to herbeviruses. Virus isolation attempts of LAKV failed. BKAV is most closely related to the bird-associated orthobunyaviruses Koongol virus and Gamboa virus. BKAV was successfully isolated in mosquito cells but did not replicate in common mammalian cells from various species and organs. Also cells derived from chicken were not susceptible. Interestingly, BKAV can infect cells derived from a duck species that is endemic in the region where the BKAV-positive mosquito was collected. These results suggest a narrow host specificity and maintenance in a mosquito–bird transmission cycle. View Full-Text
Keywords: Peribunyaviridae; orthobunyavirus; arbovirus; insect-specific virus; mosquito; Mexico Peribunyaviridae; orthobunyavirus; arbovirus; insect-specific virus; mosquito; Mexico
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Kopp, A.; Hübner, A.; Zirkel, F.; Hobelsberger, D.; Estrada, A.; Jordan, I.; Gillespie, T.R.; Drosten, C.; Junglen, S. Detection of Two Highly Diverse Peribunyaviruses in Mosquitoes from Palenque, Mexico. Viruses 2019, 11, 832.

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