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Article

Specificity of Morbillivirus Hemagglutinins to Recognize SLAM of Different Species

1
Center for Research and Education on Drug Discovery, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan
2
Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan
3
Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan
4
Department of Virology 3, National Institute of Infectious Diseases, Tokyo 208-0011, Japan
5
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan
6
Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 4-1-8, Honcho Kawaguchi, Saitama 332-0012, Japan
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Viruses 2019, 11(8), 761; https://doi.org/10.3390/v11080761
Received: 31 May 2019 / Revised: 15 August 2019 / Accepted: 16 August 2019 / Published: 19 August 2019
(This article belongs to the Special Issue Morbilliviruses)
Measles virus (MV) and canine distemper virus (CDV) are highly contagious and deadly, forming part of the morbillivirus genus. The receptor recognition by morbillivirus hemagglutinin (H) is important for determining tissue tropism and host range. Recent reports largely urge caution as regards to the potential expansion of host specificities of morbilliviruses. Nonetheless, the receptor-binding potential in different species of morbillivirus H proteins is largely unknown. Herein, we show that the CDV-H protein binds to the dog signaling lymphocyte activation molecule (SLAM), but not to the human, tamarin, or mouse SLAM. In contrast, MV-H can bind to human, tamarin and dog SLAM, but not to that of mice. Notably, MV binding to dog SLAM showed a lower affinity and faster kinetics than that of human SLAM, and MV exhibits a similar entry activity in dog SLAM- and human SLAM-expressing Vero cells. The mutagenesis study using a fusion assay, based on the MV-H–SLAM complex structure, revealed differences in tolerance for the receptor specificity between MV-H and CDV-H. These results provide insights into H-SLAM specificity related to potential host expansion. View Full-Text
Keywords: morbillivirus; hemagglutinin; SLAM; canine distemper virus; measles virus; surface plasmon resonance; structure morbillivirus; hemagglutinin; SLAM; canine distemper virus; measles virus; surface plasmon resonance; structure
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MDPI and ACS Style

Fukuhara, H.; Ito, Y.; Sako, M.; Kajikawa, M.; Yoshida, K.; Seki, F.; Mwaba, M.H.; Hashiguchi, T.; Higashibata, M.-a.; Ose, T.; Kuroki, K.; Takeda, M.; Maenaka, K. Specificity of Morbillivirus Hemagglutinins to Recognize SLAM of Different Species. Viruses 2019, 11, 761. https://doi.org/10.3390/v11080761

AMA Style

Fukuhara H, Ito Y, Sako M, Kajikawa M, Yoshida K, Seki F, Mwaba MH, Hashiguchi T, Higashibata M-a, Ose T, Kuroki K, Takeda M, Maenaka K. Specificity of Morbillivirus Hemagglutinins to Recognize SLAM of Different Species. Viruses. 2019; 11(8):761. https://doi.org/10.3390/v11080761

Chicago/Turabian Style

Fukuhara, Hideo, Yuri Ito, Miyuki Sako, Mizuho Kajikawa, Koki Yoshida, Fumio Seki, Mwila H. Mwaba, Takao Hashiguchi, Masa-aki Higashibata, Toyoyuki Ose, Kimiko Kuroki, Makoto Takeda, and Katsumi Maenaka. 2019. "Specificity of Morbillivirus Hemagglutinins to Recognize SLAM of Different Species" Viruses 11, no. 8: 761. https://doi.org/10.3390/v11080761

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