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10.3390/v11080759
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Article

A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum

by
Ayesha Azhar
1,2,
Fan Mu
1,2,
Huang Huang
1,2,
Jiasen Cheng
1,2,
Yanping Fu
2,
Muhammad Rizwan Hamid
1,2,
Daohong Jiang
1,2 and
Jiatao Xie
1,2,*
1
State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China
2
Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(8), 759; https://doi.org/10.3390/v11080759
Submission received: 16 May 2019 / Revised: 12 August 2019 / Accepted: 15 August 2019 / Published: 16 August 2019
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

Infection by diverse mycoviruses is a common phenomenon in Sclerotinia sclerotiorum. In this study, the full genome of a single-stranded RNA mycovirus, tentatively named Hubei sclerotinia RNA virus 1 (HuSRV1), was determined in the hypovirulent strain 277 of S. sclerotiorum. The HuSRV1 genome is 4492 nucleotides (nt) long and lacks a poly (A) tail at the 3ˊ- terminus. Sequence analyses showed that the HuSRV1 genome contains four putative open reading frames (ORFs). ORF1a was presumed to encode a protein with a conserved protease domain and a transmembrane domain. This protein is 27% identical to the P2a protein encoded by the subterranean clover mottle virus. ORF1b encodes a protein containing a conserved RNA-dependent RNA polymerase (RdRp) domain, which may be translated into a fusion protein by a -1 ribosome frameshift. This protein is 45.9% identical to P2b encoded by the sowbane mosaic virus. ORF2 was found to encode a putative coat protein, which shares 23% identical to the coat protein encoded by the olive mild mosaic virus. ORF3 was presumed to encode a putative protein with an unknown function. Evolutionary relation analyses indicated that HuSRV1 is related to members within Sobemovirus, but forms a unique phylogenetic branch, suggesting that HuSRV1 represents a new member within Solemoviridae. HuSRV1 virions, approximately 30 nm in diameter, were purified from strain 277. The purified virions were successfully introduced into virulent strain Ep-1PNA367, resulting in a new hypovirulent strain, which confirmed that HuSRV1 confers hypovirulence on S. sclerotiorum.
Keywords: Sclerotinia sclerotiorum; mycovirus; plant virus; Sobemovirus Sclerotinia sclerotiorum; mycovirus; plant virus; Sobemovirus

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MDPI and ACS Style

Azhar, A.; Mu, F.; Huang, H.; Cheng, J.; Fu, Y.; Hamid, M.R.; Jiang, D.; Xie, J. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses 2019, 11, 759. https://doi.org/10.3390/v11080759

AMA Style

Azhar A, Mu F, Huang H, Cheng J, Fu Y, Hamid MR, Jiang D, Xie J. A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses. 2019; 11(8):759. https://doi.org/10.3390/v11080759

Chicago/Turabian Style

Azhar, Ayesha, Fan Mu, Huang Huang, Jiasen Cheng, Yanping Fu, Muhammad Rizwan Hamid, Daohong Jiang, and Jiatao Xie. 2019. "A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum" Viruses 11, no. 8: 759. https://doi.org/10.3390/v11080759

APA Style

Azhar, A., Mu, F., Huang, H., Cheng, J., Fu, Y., Hamid, M. R., Jiang, D., & Xie, J. (2019). A Novel RNA Virus Related to Sobemoviruses Confers Hypovirulence on the Phytopathogenic Fungus Sclerotinia sclerotiorum. Viruses, 11(8), 759. https://doi.org/10.3390/v11080759

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