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Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV)

1
Vector Borne Diseases Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, Delhi 110067, India
2
Animal House Facility, International Centre for Genetic Engineering and Biotechnology, New Delhi, Delhi 110067, India
*
Author to whom correspondence should be addressed.
Current address: Department of Human Retrovirology, Montreal Clinical Research Institute, Montreal, QC H2W 1R7, Canada.
These authors contributed equally to this work.
Viruses 2019, 11(6), 578; https://doi.org/10.3390/v11060578
Received: 1 March 2019 / Revised: 18 May 2019 / Accepted: 20 May 2019 / Published: 25 June 2019
(This article belongs to the Special Issue Chikungunya Virus and (Re-) Emerging Alphaviruses)
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Abstract

Chikungunya (CHIK) is a febrile arboviral illness caused by chikungunya virus (CHIKV) and has been identified in more than 60 countries across the globe. A major public health concern, the infection occurs as an acute febrile phase and a chronic arthralgic phase. The disease manifests differently in different age groups that can range from asymptomatic infection in the younger age group to a prolonged chronic phase in the elderly population. The present study was undertaken to evaluate strain-specific pathogenesis of ECSA genotype of CHIKV strains derived from clinical isolates in adult C57BL/6J mice model. The strain that was pathogenic and developed distinct acute and post–acute phase of CHIK infection was further evaluated for dose-dependent pathogenesis. Upon arriving on the optimal dose to induce clinical symptoms in the mice, the disease progression was evaluated across the acute and the post–acute phase of infection for a period of 15 days post–infection in two age groups of mice, namely eight weeks old and 20 weeks old mice groups. Biochemical, hematological, and virology attributes were measured and correlated to morbidity and linked neurotropism and limb thickness in the two age groups. Our results show that CHIKV exhibit strain-specific pathogenesis in C57BL/6J mice. Distinct dissimilarities were observed between the two age groups in terms of pathogenesis, viral clearance and host response to CHIKV infection. View Full-Text
Keywords: chikungunya virus; animal model; pathogenicity; C57BL/6J mice; disease progression chikungunya virus; animal model; pathogenicity; C57BL/6J mice; disease progression
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Jain, J.; Narayanan, V.; Kumar, A.; Shrinet, J.; Srivastava, P.; Chaturvedi, S.; Sunil, S. Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV). Viruses 2019, 11, 578.

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