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RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection

1
CSIRO Health & Biosecurity, Australian Animal Health Laboratory, Geelong 3220, Australia
2
School of Applied Sciences, RMIT University, Bundoora 3083, Australia
3
School of Health and Biomedical Science, RMIT University, Bundoora 3083, Australia
*
Author to whom correspondence should be addressed.
Current Address: Mosquitoes and Emerging Arboviruses, Institut Pasteur de la Guyane, Cayenne 97306, French Guiana.
Viruses 2019, 11(6), 513; https://doi.org/10.3390/v11060513
Received: 4 May 2019 / Accepted: 2 June 2019 / Published: 4 June 2019
(This article belongs to the Special Issue Chikungunya Virus and (Re-) Emerging Alphaviruses)
Chikungunya virus (CHIKV) is an emerging pathogen around the world and causes significant morbidity in patients. A single amino acid mutation in the envelope protein of CHIKV has led to a shift in vector preference towards Aedes albopictus. While mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. We performed whole transcriptome analysis on dissected midguts of Aedes albopictus infected with CHIKV to identify differentially expressed genes. For this, RNA was extracted at two days post-infection (2-dpi) from pooled midguts. We initially identified 25 differentially expressed genes (p-value < 0.05) when mapped to a reference transcriptome. Further, multiple differentially expressed genes were identified from a custom de novo transcriptome, which was assembled using the reads that did not align with the reference genome. Thirteen of the identified transcripts, possibly involved in immunity, were validated by qRT-PCR. Homologues of seven of these genes were also found to be significantly upregulated in Aedes aegypti midguts 2 dpi, indicating a conserved mechanism at play. These results will help us to characterise the molecular interaction between Aedes albopictus and CHIKV and can be utilised to reduce the impact of this viral infection. View Full-Text
Keywords: Chikungunya; Aedes albopictus; RNASeq; Host–pathogen interactions Chikungunya; Aedes albopictus; RNASeq; Host–pathogen interactions
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Vedururu, R.; Neave, M.J.; Tachedjian, M.; Klein, M.J.; Gorry, P.R.; Duchemin, J.-B.; Paradkar, P.N. RNASeq Analysis of Aedes albopictus Mosquito Midguts after Chikungunya Virus Infection. Viruses 2019, 11, 513.

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