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Vector Competence of Culicoides sonorensis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype 2 Strains from Canada and Florida

Florida Medical Entomology Laboratory, University of Florida, 200 9th St. SE, Vero Beach, FL 32962, USA
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Viruses 2019, 11(4), 367; https://doi.org/10.3390/v11040367
Received: 28 March 2019 / Revised: 18 April 2019 / Accepted: 20 April 2019 / Published: 22 April 2019
(This article belongs to the Special Issue Virus-Vector-Host Interactions of Culicoides-Borne Diseases)
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Abstract

Epizootic hemorrhagic disease virus (EHDV), an Orbivirus transmitted by Culicoides spp. vectors, is represented by seven serotypes and numerous strains worldwide. While studies comparing vector competence between serotypes exist, studies between viral strains are lacking. In this study, we examined the rates of infection, dissemination, and transmission of two strains of EHDV-2 orally fed to the known vector, Culicoides sonorensis Wirth & Jones. Culicoides sonorensis cohorts were fed an infectious blood meal containing EHDV-2 strains from either Alberta, Canada (Can-Alberta) or Florida (5.5 log10 PFUe/mL) and tested for the vector’s susceptibility to infection and dissemination. In addition, transmission rates of the virus were assessed and compared using capillary tube and honey card methods. Our results show that the Florida strain had higher infection and dissemination rates than the Can-Alberta strain in spite of the Florida strain having significantly lower viral titers in C. sonorensis bodies, legs, and saliva than the Can-Alberta strain. Overall transmission rates were not significantly different between the two strains but varied significantly between the methods used. These findings suggest that the consequences of EHDV infection in C. sonorensis vary between virus strains and have huge implications in future vector competence studies involving Culicoides species and Orbiviruses. View Full-Text
Keywords: Culicoides sonorensis; epizootic hemorrhagic disease virus; EHDV-2; vector competence; strain; infection; dissemination; transmission Culicoides sonorensis; epizootic hemorrhagic disease virus; EHDV-2; vector competence; strain; infection; dissemination; transmission
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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McGregor, B.L.; Erram, D.; Acevedo, C.; Alto, B.W.; Burkett-Cadena, N.D. Vector Competence of Culicoides sonorensis (Diptera: Ceratopogonidae) for Epizootic Hemorrhagic Disease Virus Serotype 2 Strains from Canada and Florida. Viruses 2019, 11, 367.

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