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Characterization of the RNA Transcription Profile of Bombyx mori Bidensovirus

Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China
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Viruses 2019, 11(4), 325; https://doi.org/10.3390/v11040325
Received: 19 March 2019 / Revised: 29 March 2019 / Accepted: 30 March 2019 / Published: 3 April 2019
(This article belongs to the Special Issue New Insights into Parvovirus Research)
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Abstract

Bombyx mori bidensovirus (BmBDV) is a single-stranded DNA (ssDNA) virus from the genus Bidensovirus of the Bidnaviridae family, which, thus far, solely infects insects. It has a unique genome that contains bipartite DNA molecules (VD1 and VD2). In this study, we explored the detailed transcription mapping of the complete BmBDV genome (VD1 and VD2) by rapid amplification of cDNA ends (RACE), reverse transcription quantitative real-time PCR (RT-qPCR), and luciferase assays. For the first time, we report the transcription map of VD2. Our mapping of the transcriptional start sites reveals that the NS genes in VD1 have separate transcripts that are derived from overlapping promoters, P5 and P5.5. Thus, our study provides a strategy for alternative promoter usage in the expression of BmBDV genes. View Full-Text
Keywords: Bombyx mori bidensovirus; RACE; RT-qPCR; transcription mapping; overlapping promoters Bombyx mori bidensovirus; RACE; RT-qPCR; transcription mapping; overlapping promoters
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Li, R.; Chang, P.; Lü, P.; Hu, Z.; Chen, K.; Yao, Q.; Yu, Q. Characterization of the RNA Transcription Profile of Bombyx mori Bidensovirus. Viruses 2019, 11, 325.

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