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Open AccessArticle

Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus

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Unité Épidémiologie et Physiopathologie des Virus Oncogènes, UMR Centre National de la Recherche Scientifique 3569, Département Virologie, Institut Pasteur, 75015 Paris, France
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Université de Paris, 75013 Paris, France
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Groupe à 5 ans Plasticité cellulaire et Modélisation des Maladies, Département Biologie du Développement et cellules souches, Institut Pasteur, 75015 Paris, France
*
Authors to whom correspondence should be addressed.
Viruses 2019, 11(10), 950; https://doi.org/10.3390/v11100950
Received: 12 September 2019 / Revised: 8 October 2019 / Accepted: 10 October 2019 / Published: 15 October 2019
(This article belongs to the Section Animal Viruses)
Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk of infected mothers, and the existence of breastfeeding as a new mode of mother-to-child transmission of ZIKV was recently hypothesized. However, the maternal origin of infectious particles in breast milk is currently unknown. Here, we show that ZIKV disseminates to the mammary glands of infected mice after both systemic and local exposure with differential kinetics. Ex vivo, we demonstrate that primary human mammary epithelial cells were sensitive and permissive to ZIKV infection in this study. Moreover, by using in vitro models, we prove that mammary luminal- and myoepithelial-phenotype cell lines are both able to produce important virus progeny after ZIKV exposure. Our data suggest that the dissemination of ZIKV to the mammary glands and subsequent infection of the mammary epithelium could be one mechanism of viral excretion in human breast milk. View Full-Text
Keywords: Zika virus; dissemination; mammary glands; tropism; primary cells; luminal cells; myoepithelial cells Zika virus; dissemination; mammary glands; tropism; primary cells; luminal cells; myoepithelial cells
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MDPI and ACS Style

Hubert, M.; Chiche, A.; Legros, V.; Jeannin, P.; Montange, T.; Gessain, A.; Ceccaldi, P.-E.; Vidy, A. Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus. Viruses 2019, 11, 950.

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