Viruses 2019, 11(1), 87; https://doi.org/10.3390/v11010087
Aerosol and Contact Transmission Following Intranasal Infection of Mice with Japanese Encephalitis Virus
Chunxia Chai 1,†, Rachel Palinski 2,†, Yixuan Xu 1,†
, Qiao Wang 1, Sanjie Cao 1, Yi Geng 3, Qin Zhao 1, Yiping Wen 1, Xiaobo Huang 1, Qiguai Yan 1, Xiaoping Ma 1, Xintian Wen 1, Yong Huang 1, Xinfeng Han 1, Wenjun Ma 2,* and Rui Wu 1,3,*
, Qiao Wang 1, Sanjie Cao 1, Yi Geng 3, Qin Zhao 1, Yiping Wen 1, Xiaobo Huang 1, Qiguai Yan 1, Xiaoping Ma 1, Xintian Wen 1, Yong Huang 1, Xinfeng Han 1, Wenjun Ma 2,* and Rui Wu 1,3,*
1
Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
2
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA
3
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Received: 25 October 2018 / Revised: 8 January 2019 / Accepted: 18 January 2019 / Published: 21 January 2019
(This article belongs to the Section Animal Viruses)
Abstract
The Japanese encephalitis virus (JEV), a causative agent of severe viral encephalitis in humans, has a biological cycle fluctuating between transmission in mosquitoes and avian species and amplification in pigs. Contact transmission of JEV was recently shown in pigs in the absence of arthropod vectors. Here, we show JEV transmission between infected and contact mice and further demonstrate that JEV transmission occurs between animals via aerosols, as both viral RNA and infectious JEV were detected in direct contact- and aerosol-exposed contact animals. The results of this study change our understanding of JEV transmission in densely populated regions and may help to explain JEV outbreaks without the presence of arthropod vectors. View Full-TextKeywords:
JEV; contact transmission; aerosol transmission; intranasal infection; mice
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MDPI and ACS Style
Chai, C.; Palinski, R.; Xu, Y.; Wang, Q.; Cao, S.; Geng, Y.; Zhao, Q.; Wen, Y.; Huang, X.; Yan, Q.; Ma, X.; Wen, X.; Huang, Y.; Han, X.; Ma, W.; Wu, R. Aerosol and Contact Transmission Following Intranasal Infection of Mice with Japanese Encephalitis Virus. Viruses 2019, 11, 87.
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