Next Article in Journal
Aerosol and Contact Transmission Following Intranasal Infection of Mice with Japanese Encephalitis Virus
Previous Article in Journal
The Incorporation of Host Proteins into the External HIV-1 Envelope
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Viruses 2019, 11(1), 86; https://doi.org/10.3390/v11010086

Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections

1
State Key laboratory of Agricultural Microbiology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2
The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China
3
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture), Animal Husbandry and Veterinary Institute, Hubei Academy of Agricultural Science, Wuhan 430064, China
*
Authors to whom correspondence should be addressed.
Received: 6 November 2018 / Revised: 15 January 2019 / Accepted: 18 January 2019 / Published: 21 January 2019
(This article belongs to the Section Bacterial Viruses)
Full-Text   |   PDF [5713 KB, uploaded 21 January 2019]   |  
  |   Review Reports

Abstract

A lytic bacteriophage PHB01 specific for Pasteurella multocida type D was isolated from the sewage water collected from a pig farm. This phage had the typical morphology of the family Podoviridae, order Caudovirales, presenting an isometric polyhedral head and a short noncontractile tail. PHB01 was able to infect most of the non-toxigenic P. multocida type D strains tested, but not toxigenic type D strains and those belonging to other capsular types. Phage PHB01, the first lytic phage specific for P. multocida type D sequenced thus far, presents a 37,287-bp double-stranded DNA genome with a 223-bp terminal redundancy. The PHB01 genome showed the highest homology with that of PHB02, a lytic phage specific for P. multocida type A. Phylogenetic analysis showed that PHB01 and PHB02 were composed of a genus that was close to the T7-virus genus. In vivo tests using mouse models showed that the administration of PHB01 was safe to the mice and had a good effect on treating the mice infected with different P. multocida type D strains including virulent strain HN05. These findings suggest that PHB01 has a potential use in therapy against infections caused by P. multocida type D. View Full-Text
Keywords: bacteriophage; lytic; P. multocida type D; isolation; therapeutic application bacteriophage; lytic; P. multocida type D; isolation; therapeutic application
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Chen, Y.; Guo, G.; Sun, E.; Song, J.; Yang, L.; Zhu, L.; Liang, W.; Hua, L.; Peng, Z.; Tang, X.; Chen, H.; Wu, B. Isolation of a T7-Like Lytic Pasteurella Bacteriophage vB_PmuP_PHB01 and Its Potential Use in Therapy against Pasteurella multocida Infections. Viruses 2019, 11, 86.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top