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Viruses 2018, 10(9), 488; https://doi.org/10.3390/v10090488

Functional Scanning of Apple Geminivirus Proteins as Symptom Determinants and Suppressors of Posttranscriptional Gene Silencing

1
State Key Laboratory for Plant Disease and Insect Pest, Institute of Plant protection, China Academy of Agricultural Sciences, Beijing 100193, China
2
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China
*
Authors to whom correspondence should be addressed.
Received: 30 July 2018 / Revised: 26 August 2018 / Accepted: 7 September 2018 / Published: 11 September 2018
(This article belongs to the Special Issue Plant-Virus Interactions)
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Abstract

Apple geminivirus (AGV) is a recently identified geminivirus which is isolated from the apple tree in China. We carried out functional scanning of apple geminivirus proteins as symptom determinants and suppressors of posttranscriptional gene silencing (PTGS). Our results indicated that AGV V2 is an important virulence factor localized to the nucleus and cytoplasm that suppresses PTGS and induces severe symptoms of crinkling and necrosis. AGV C1 is also a virulence determinant which elicits systemic necrosis when expressed from a PVX-based vector. The AGV C4 is targeted to cytoplasm, plasma membrane, nucleus, and chloroplasts. The inoculation of PVX-C4 on N. benthamiana induced severe upward leaf curling, which implied that AGV C4 also functions as a symptom determinant, and mutation analyses suggested that the acylated residues on Gly2 and Cys8 play important roles in its subcellular localization and symptom development. View Full-Text
Keywords: Apple geminivirus; RNA silencing suppressor; symptom determinant Apple geminivirus; RNA silencing suppressor; symptom determinant
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhan, B.; Zhao, W.; Li, S.; Yang, X.; Zhou, X. Functional Scanning of Apple Geminivirus Proteins as Symptom Determinants and Suppressors of Posttranscriptional Gene Silencing. Viruses 2018, 10, 488.

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