Next Article in Journal
Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea
Next Article in Special Issue
Strategies to Encapsulate the Staphylococcus aureus Bacteriophage phiIPLA-RODI
Previous Article in Journal
Molecular and Biological Characterisation of Turnip mosaic virus Isolates Infecting Poppy (Papaver somniferum and P. rhoeas) in Slovakia
Previous Article in Special Issue
Structure and Analysis of R1 and R2 Pyocin Receptor-Binding Fibers
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessCommunication

Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens

Physical Biochemistry, University of Potsdam, 14476 Potsdam, Germany
Institut Pasteur, Unité de Chimie des Biomolécules, 28 rue du Roux, 75015 Paris, France
CNRS UMR 3523, Institut Pasteur, 75015 Paris, France
National Reference Centre for Salmonella and other Bacterial Enterics, Robert Koch Institute, 38855 Wernigerode, Germany
Author to whom correspondence should be addressed.
Current address: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK.
Viruses 2018, 10(8), 431;
Received: 29 May 2018 / Revised: 26 July 2018 / Accepted: 9 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Biotechnological Applications of Phage and Phage-Derived Proteins)
PDF [2361 KB, uploaded 15 August 2018]


Bacteriophage research is gaining more importance due to increasing antibiotic resistance. However, for treatment with bacteriophages, diagnostics have to be improved. Bacteriophages carry adhesion proteins, which bind to the bacterial cell surface, for example tailspike proteins (TSP) for specific recognition of bacterial O-antigen polysaccharide. TSP are highly stable proteins and thus might be suitable components for the integration into diagnostic tools. We used the TSP of bacteriophage Sf6 to establish two applications for detecting Shigella flexneri (S. flexneri), a highly contagious pathogen causing dysentery. We found that Sf6TSP not only bound O-antigen of S. flexneri serotype Y, but also the glucosylated O-antigen of serotype 2a. Moreover, mass spectrometry glycan analyses showed that Sf6TSP tolerated various O-acetyl modifications on these O-antigens. We established a microtiter plate-based ELISA like tailspike adsorption assay (ELITA) using a Strep-tag®II modified Sf6TSP. As sensitive screening alternative we produced a fluorescently labeled Sf6TSP via coupling to an environment sensitive dye. Binding of this probe to the S. flexneri O-antigen Y elicited a fluorescence intensity increase of 80% with an emission maximum in the visible light range. The Sf6TSP probes thus offer a promising route to a highly specific and sensitive bacteriophage TSP-based Shigella detection system. View Full-Text
Keywords: Shigella flexneri; bacteriophage; tailspike proteins; O-antigen; serotyping; microtiter plate assay; fluorescence sensor Shigella flexneri; bacteriophage; tailspike proteins; O-antigen; serotyping; microtiter plate assay; fluorescence sensor

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Kunstmann, S.; Scheidt, T.; Buchwald, S.; Helm, A.; Mulard, L.A.; Fruth, A.; Barbirz, S. Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens. Viruses 2018, 10, 431.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top