Next Article in Journal
Potential Therapeutic Agents for Feline Calicivirus Infection
Next Article in Special Issue
Capsid Structure of dsRNA Fungal Viruses
Previous Article in Journal
Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens
Previous Article in Special Issue
Two Novel Hypovirulence-Associated Mycoviruses in the Phytopathogenic Fungus Botrytis cinerea: Molecular Characterization and Suppression of Infection Cushion Formation
Open AccessCommunication

Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea

by Fangmin Hao 1,2, Mingde Wu 1,2,* and Guoqing Li 1,2
1
The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(8), 432; https://doi.org/10.3390/v10080432
Received: 24 July 2018 / Revised: 12 August 2018 / Accepted: 14 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Mycoviruses)
Here, we characterized a negative single-stranded (−ss)RNA mycovirus, Botrytis cinerea mymonavirus 1 (BcMyV1), isolated from the phytopathogenic fungus Botrytis cinerea. The genome of BcMyV1 is 7863 nt in length, possessing three open reading frames (ORF1–3). The ORF1 encodes a large polypeptide containing a conserved mononegaviral RNA-dependent RNA polymerase (RdRp) domain showing homology to the protein L of mymonaviruses, whereas the possible functions of the remaining two ORFs are still unknown. The internal cDNA sequence (10-7829) of BcMyV1 was 97.9% identical to the full-length cDNA sequence of Sclerotinia sclerotiorum negative stranded RNA virus 7 (SsNSRV7), a virus-like contig obtained from Sclerotinia sclerotiorum metatranscriptomes, indicating BcMyV1 should be a strain of SsNSRV7. Phylogenetic analysis based on RdRp domains showed that BcMyV1 was clustered with the viruses in the family Mymonaviridae, suggesting it is a member of Mymonaviridae. BcMyV1 may be widely distributed in regions where B. cinerea occurs in China and even over the world, although it infected only 0.8% of tested B. cinerea strains. View Full-Text
Keywords: Botrytis cinerea; Botrytis cinerea mymonavirus 1; Mymonaviridae Botrytis cinerea; Botrytis cinerea mymonavirus 1; Mymonaviridae
Show Figures

Figure 1

MDPI and ACS Style

Hao, F.; Wu, M.; Li, G. Molecular Characterization and Geographic Distribution of a Mymonavirus in the Population of Botrytis cinerea. Viruses 2018, 10, 432.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop