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Viruses 2018, 10(12), 700; https://doi.org/10.3390/v10120700

Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains

1
Department of Microbiology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USA
2
Department of Microbiology, Peking University Health Science Center School of Basic Medical Sciences, Beijing 100191, China
*
Author to whom correspondence should be addressed.
Received: 14 September 2018 / Revised: 1 December 2018 / Accepted: 5 December 2018 / Published: 9 December 2018
(This article belongs to the Special Issue New Advances on Zika Virus Research)
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Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus that has emerged as an important human viral pathogen, causing congenital malformation including microcephaly among infants born to mothers infected with the virus during pregnancy. Phylogenetic analysis suggested that ZIKV can be classified into African and Asian lineages. In this study, we have developed a stable plasmid-based reverse genetic system for robust production of both ZIKV prototype African-lineage MR766 and clinical Asian-lineage FSS13025 strains using a tetracycline (Tet)-controlled gene expression vector. Transcription of the full-length ZIKV RNA is under the control of the Tet-responsive Ptight promoter at the 5′ end and an antigenomic ribozyme of hepatitis delta virus at the 3′ end. The transcription of infectious ZIKV RNA genome was efficiently induced by doxycycline. This novel ZIKV reverse genetics system will be valuable for the study of molecular viral pathogenesis of ZIKV and the development of new vaccines against ZIKV infection. View Full-Text
Keywords: Zika virus; reverse genetics; infectious cDNA; Tet-inducible; MR766; FSS13025 Zika virus; reverse genetics; infectious cDNA; Tet-inducible; MR766; FSS13025
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Zhang, L.; Ji, W.; Lyu, S.; Qiao, L.; Luo, G. Tet-Inducible Production of Infectious Zika Virus from the Full-Length cDNA Clones of African- and Asian-Lineage Strains. Viruses 2018, 10, 700.

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