Next Article in Journal
The Main Risk Factors of Nipah Disease and Its Risk Analysis in China
Next Article in Special Issue
Silvestrol Inhibits Chikungunya Virus Replication
Previous Article in Journal
Rapid Construction of a Replication-Competent Infectious Clone of Human Adenovirus Type 14 by Gibson Assembly
Open AccessArticle

Ultrastructural Analysis of Chikungunya Virus Dissemination from the Midgut of the Yellow Fever Mosquito, Aedes aegypti

Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(10), 571; https://doi.org/10.3390/v10100571
Received: 21 September 2018 / Revised: 15 October 2018 / Accepted: 16 October 2018 / Published: 18 October 2018
(This article belongs to the Special Issue Chikungunya Virus and (Re-) Emerging Alphaviruses)
The transmission cycle of chikungunya virus (CHIKV) requires that mosquito vectors get persistently infected with the virus, following its oral acqsuisition from a vertebrate host. The mosquito midgut is the initial organ that gets infected with orally acquired CHIKV. Following its replication in the midgut epithelium, the virus exits the midgut and infects secondary tissues including the salivary glands before being transmitted to another host. Here, we investigate the pattern of CHIKV dissemination from the midgut of Aedes aegypti at the ultrastructural level. Bloodmeal ingestion caused overstretching of the midgut basal lamina (BL), which was disrupted in areas adjacent to muscles surrounding the midgut as shown by scanning electron microscopy (SEM). Using both transmission electron microscopy (TEM) and focused ion beam scanning electron microscopy (FIB-SEM) to analyze midgut preparations, mature chikungunya (CHIK) virions were found accumulating at the BL and within strands of the BL at 24–32 h post-infectious bloodmeal (pibm). From 48 h pibm onwards, virions no longer congregated at the BL and became dispersed throughout the basal labyrinth of the epithelial cells. Ingestion of a subsequent, non-infectious bloodmeal caused mature virions to congregate again at the midgut BL. Our study suggests that CHIKV needs a single replication cycle in the midgut epithelium before mature virions directly traverse the midgut BL during a relatively narrow time window, within 48 h pibm. View Full-Text
Keywords: chikungunya virus; mosquito; midgut; dissemination; ultrastructural; FIB-SEM; electron microscopy; Mayaro virus; bloodmeal; basal lamina chikungunya virus; mosquito; midgut; dissemination; ultrastructural; FIB-SEM; electron microscopy; Mayaro virus; bloodmeal; basal lamina
Show Figures

Graphical abstract

MDPI and ACS Style

Kantor, A.M.; Grant, D.G.; Balaraman, V.; White, T.A.; Franz, A.W.E. Ultrastructural Analysis of Chikungunya Virus Dissemination from the Midgut of the Yellow Fever Mosquito, Aedes aegypti. Viruses 2018, 10, 571.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop