Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems
Abstract
:1. Introduction
2. Materials and Methods
2.1. Site Description
2.2. CH4 Flux Measurements
2.3. Determination of the Soil Methane Oxidation Rate
2.4. Methane Concentration
2.5. Soil Chemical and Physical Properties
2.6. Statistical Analysis
2.7. Soil DNA Extraction
2.8. Illumina Sequencing and Analysis of 16S rRNA Gene Fragments
2.9. Illumina Sequencing and Analysis of pmoA Gene Fragments
3. Results and Discussion
3.1. Methane Fluxes
3.2. Soil Methane Oxidation Rates
3.3. Prokaryote Diversity Patterns in Forest Soils
3.4. Identification of Methanotrophs Based on 16S rRNA Gene Analysis
3.5. PmoA-Based Identification of Methanotrophic Bacteria
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Forest Ecosystem | Coordinates | Bulk Density, g cm−3 | Litter Thickness, cm | pH | Corg, % | NH4+, µgN gDW−1 | NO3−, µgN gDW−1 |
---|---|---|---|---|---|---|---|
Aspen | 61.05623° N 69.42942° E | 0.84 ± 0.08 | 2 ± 1 | 5.8 ± 0.2 | 2.5 ± 0.4 | 7.1 ± 1.4 | 121.0 ± 9.8 |
Siberian pine | 61.08571° N 69.46918° E | 0.99 ± 0.05 | 1 ± 1 | 5.1 ± 0.2 | 1.4 ± 0.4 | 12.9 ± 6.1 | 93.2 ± 23.6 |
Mixed | 61.08301° N 69.45383° E | 1.05 ± 0.06 | 1 ± 1 | 5.4 ± 0.2 | 1.8 ± 0.3 | 9.8 ± 3.9 | 151.4 ± 68.9 |
Parameter | Season | Ecosystem | Depth |
---|---|---|---|
Flux | 0.72 *** | 0.03 ** | NM |
CH4 oxidation rate | 0.29 *** | NS | 0.48 *** |
Soil moisture (by volume) | 0.46 *** | 0.05 *** | 0.08 *** |
Water-filled pore space | 0.27 *** | 0.03 ** | 0.39 *** |
CH4 concentration | 0.25 *** | 0.02 ** | 0.45 *** |
Soil Temperature | 0.90 *** | 0.01 *** | 0.05 *** |
pH | NS | 0.17 ** | NS |
NH4+ | 0.24 ** | NS | 0.16 ** |
NO3− | 0.40 *** | 0.12 ** | NS |
Methanotrophs abundance a | 0.04 * | NS | 0.53 *** |
OTU ID | Relative Abundance (%) | Taxonomy | Closest Silva Match | Habitat | |
---|---|---|---|---|---|
Siberian Pine Forest | SF-16S-1 | 5.71 | Bradyrhizobium sp. 175LB2PYPT | HQ698291 | Root nodules of Acacia pycnantha |
SF-16S-2 | 3.92 | Uncultured WD260 group of Gammaproteobacteria | EU150272 | Boreal pine forest soil | |
SF-16S-3 | 3.02 | Uncultured RCP2-54 bacterium | DQ451494 | Forest soil | |
SF-16S-4 | 2.75 | Uncultured WD260 group of Gammaproteobacteria | EU150268 | Soil from Niwot Ridge LTER | |
SF-16S-5 | 2.73 | Mycobacterium celatum MB72 | AJ416914 | AIDS patients | |
SF-16S-6 | 2.58 | Acidobacteriales (Sd 1) uncultured | HQ598256 | Woodland soil | |
SF-16S-7 | 2.43 | Acidobacteriales (Sd 1) uncultured | FJ624925 | Boreal pine forest soil | |
SF-16S-8 | 2.29 | Acidobacteriales (Sd 1) uncultured | AY963436 | Soil of evergreen broad-leaved forest | |
SF-16S-9 | 2.13 | Bradyrhizobium sp. | JX644393 | Earthworm nephridia | |
SF-16S-10 | 2.13 | Acidobacteriae (Sd 2) uncultured | FJ466148 | Acidic fen soil | |
Mixed Forest | MF-16S-1 | 6.22 | Bradyrhizobium sp. 175LB2PYPT | HQ698291 | Root nodules of Acacia pycnantha |
MF-16S-2 | 5.43 | Solirubrobacteraceae uncultured | HM270154 | Tobacco rhizosphere | |
MF-16S-3 | 2.54 | Uncultured WD260 group of Gammaproteobacteria | EU150268 | Soil from Niwot Ridge LTER | |
MF-16S-4 | 2.02 | Mycobacterium sp. MPLK-65 | KX689762 | Subterranean mine | |
MF-16S-5 | 1.80 | Uncultured WD260 group of Gammaproteobacteria | AB991083 | Temperate highland grassland | |
MF-16S-6 | 1.79 | Mycobacterium celatum MB72 | AJ416914 | AIDS patients | |
MF-16S-7 | 1.27 | Conexibacter sp. uncultured | HM263196 | Grassland soil | |
MF-16S-8 | 1.09 | Xanthobacteraceae uncultured | AY963361 | Poplar tree rhizosphere | |
MF-16S-9 | 1.01 | Roseiarcus sp. | AY425766 | Tobacco rhizosphere | |
MF-16S-10 | 0.99 | Uncultured WD260 group of Gammaproteobacteria | EU150272 | Boreal pine forest soil | |
Aspen Forest | AF-16S-1 | 9.10 | Bradyrhizobium sp. 175LB2PYPT | HQ698291 | Root nodules of Acacia pycnantha |
AF-16S-2 | 3.86 | Mycobacterium sp. MPLK-65 | KX689762 | Subterranean mine | |
AF-16S-3 | 3.15 | Uncultured WD260 group of Gammaproteobacteria | AB991083 | Temperate highland grassland | |
AF-16S-4 | 2.91 | Mycobacterium celatum MB72 | AJ416914 | AIDS patients | |
AF-16S-5 | 2.32 | Uncultured WD260 group of Gammaproteobacteria | EU150268 | Soil from Niwot Ridge LTER | |
AF-16S-6 | 1.67 | Acidobacteriae (Sd 2) uncultured | AY963303 | Soil of evergreen broad-leaved forest | |
AF-16S-7 | 1.58 | Roseiarcus sp. | AY425766 | Tobacco rhizosphere | |
AF-16S-8 | 1.39 | Mycobacterium conspicuum JCM14738 | X88922 | Patients with disseminated infections | |
AF-16S-9 | 1.35 | Granulicella sp. | JN023575 | Temperate highland grassland | |
AF-16S-10 | 1.32 | Solirubrobacteraceae uncultured | KM200386 | Tobacco rhizosphere |
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Sabrekov, A.F.; Danilova, O.V.; Terentieva, I.E.; Ivanova, A.A.; Belova, S.E.; Litti, Y.V.; Glagolev, M.V.; Dedysh, S.N. Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems. Forests 2021, 12, 1738. https://doi.org/10.3390/f12121738
Sabrekov AF, Danilova OV, Terentieva IE, Ivanova AA, Belova SE, Litti YV, Glagolev MV, Dedysh SN. Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems. Forests. 2021; 12(12):1738. https://doi.org/10.3390/f12121738
Chicago/Turabian StyleSabrekov, Aleksandr F., Olga V. Danilova, Irina E. Terentieva, Anastasia A. Ivanova, Svetlana E. Belova, Yuri V. Litti, Mikhail V. Glagolev, and Svetlana N. Dedysh. 2021. "Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems" Forests 12, no. 12: 1738. https://doi.org/10.3390/f12121738