An Emerging Pathogen from Rotted Chestnut in China: Gnomoniopsis daii sp. nov.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Isolation
2.2. DNA Extraction and Phylogenetic Analysis
2.3. Morphological Identification and Characterization
2.4. Pathogenicity Trials
3. Results
3.1. Fungal Isolation and Identification
3.2. Phylogeny
3.3. Morphology and Taxonomy
3.4. Pathogenicity Trials
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Country | Host | Strain | GenBank Accession Number | ||
---|---|---|---|---|---|---|
Internal Transcribed Spacer (ITS) | tub2 | tef1 | ||||
Apiognomonia veneta | France | Platanus occidentalis | CBS 342.86 | DQ313531 | EU219235 | DQ318036 |
Gnomoniopsis alderdunensis | USA | Rubus pedatus | CBS 125679 | GU320826 | GU320788 | GU320813 |
Gnomoniopsis alderdunensis | USA | Rubus parviflorus | CBS 125680 | GU320825 | GU320787 | GU320801 |
Gnomoniopsis alderdunensis | USA | Rubus parviflorus | CBS 125681 | GU320827 | GU320789 | GU320802 |
Gnomoniopsis chamaemori | Finland | Rubus chamaemorus | CBS 804.79 | GU320817 | GU320777 | GU320809 |
Gnomoniopsis clavulata | USA | Quercus falcata | CBS 121255 | EU254818 | EU219211 | GU320807 |
Gnomoniopsis comari | Finland | Comarum palustre | CBS 806.79 | EU254821 | EU219156 | GU320810 |
Gnomoniopsis comari | Finland | Comarum palustre | CBS 807.79 | EU254822 | GU320779 | GU320814 |
Gnomoniopsis comari | Switzerland | Comarum palustre | CBS 809.79 | EU254823 | GU320778 | GU320794 |
Gnomoniopsis daii | China | Castanea mollissima | CMF002A | MN598671 | MN605519 | MN605517 |
Gnomoniopsis daii | China | Castanea mollissima | CMF002B | MN598672 | MN605520 | MN605518 |
Gnomoniopsis daii | China | Castanea mollissima | CMF095 | MN598673 | NA | NA |
Gnomoniopsis daii | China | Castanea mollissima | CMF098 | MN598674 | NA | NA |
Gnomoniopsis daii | China | Castanea mollissima | CMF099 | MN598675 | NA | NA |
Gnomoniopsis daii | China | Castanea mollissima | CMF116 | MN598676 | NA | NA |
Gnomoniopsis fructicola | USA | Fragaria vesca | CBS 121226 | EU254824 | EU219144 | GU320792 |
Gnomoniopsis fructicola | France | Fragaria sp. | CBS 208.34 | EU254826 | EU219149 | GU320808 |
Gnomoniopsis fructicola | USA | Fragaria sp. | CBS 125671 | GU320816 | GU320776 | GU320793 |
Gnomoniopsis guttulata | Bulgaria | Agrimonia eupatoria | NA | EU254812 | NA | NA |
Gnomoniopsis idaeicola | USA | Rubus sp. | CBS 125672 | GU320823 | GU320781 | GU320797 |
Gnomoniopsis idaeicola | USA | Rubus pedatus | CBS 125673 | GU320824 | GU320782 | GU320798 |
Gnomoniopsis idaeicola | France | Rubus sp. | CBS 125674 | GU320820 | GU320780 | GU320796 |
Gnomoniopsis idaeicola | USA | Rubus procerus | CBS 125675 | GU320822 | GU320783 | GU320799 |
Gnomoniopsis idaeicola | USA | Rubus procerus | CBS 125676 | GU320821 | GU320784 | GU320811 |
Gnomoniopsis macounii | USA | Spiraea sp. | CBS 121468 | EU254762 | EU219126 | GU320804 |
Gnomoniopsis occulta | USA | Potentilla sp. | CBS 125677 | GU320828 | GU320785 | GU320812 |
Gnomoniopsis occulta | USA | Potentilla sp. | CBS 125678 | GU320829 | GU320786 | GU320800 |
Gnomoniopsis occulta | Russia | Potentilla anserina | NA | EU254811 | NA | NA |
Gnomoniopsis paraclavulata | USA | Quercus alba | CBS 123202 | GU320830 | GU320775 | GU320815 |
Gnomoniopsis racemula | USA | Chamerion angustifolium | CBS 121469 | EU254841 | EU219125 | GU320803 |
Gnomoniopsis sanguisorbae | Switzerland | Sanguisorba minor | CBS 858.79 | GU320818 | GU320790 | GU320805 |
Gnomoniopsis smithogilvyi | Australia | Castanea sp. | CBS 130190 | JQ910642 | JQ910639 | KR072534 |
Gnomoniopsis smithogilvyi | Australia | Castanea sp. | CBS 130189 | JQ910644 | JQ910641 | KR072535 |
Gnomoniopsis smithogilvyi | Australia | Castanea sp. | CBS 130188 | JQ910643 | JQ910640 | KR072536 |
Gnomoniopsis smithogilvyi | Italy | Castanea sativa | MUT 401 | HM142946 | KR072532 | KR072537 |
Gnomoniopsis smithogilvyi | New Zealand | Castanea sativa | MUT 411 | HM142948 | KR072533 | KR072538 |
Gnomoniopsis tormentillae | Switzerland | Potentilla sp. | CBS 904.79 | EU254856 | EU219165 | GU320795 |
Sirococcus castaneae | Switzerland | Castanea sativa | CBS 142041 | KX929744 | KX958443 | KX929710 |
Strain | No. of Affected Nuts | No. of Asymptomatic Nuts |
---|---|---|
CMF002A | 26 | 4 |
CMF002B | 24 | 6 |
CMF095 | 22 | 8 |
CMF098 | 23 | 7 |
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Jiang, N.; Tian, C. An Emerging Pathogen from Rotted Chestnut in China: Gnomoniopsis daii sp. nov. Forests 2019, 10, 1016. https://doi.org/10.3390/f10111016
Jiang N, Tian C. An Emerging Pathogen from Rotted Chestnut in China: Gnomoniopsis daii sp. nov. Forests. 2019; 10(11):1016. https://doi.org/10.3390/f10111016
Chicago/Turabian StyleJiang, Ning, and Chengming Tian. 2019. "An Emerging Pathogen from Rotted Chestnut in China: Gnomoniopsis daii sp. nov." Forests 10, no. 11: 1016. https://doi.org/10.3390/f10111016
APA StyleJiang, N., & Tian, C. (2019). An Emerging Pathogen from Rotted Chestnut in China: Gnomoniopsis daii sp. nov. Forests, 10(11), 1016. https://doi.org/10.3390/f10111016