Differential Expression of Genes Related to the Formation of Giant Leaves in Triploid Poplar
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Total RNA Isolation, cDNA Preparation, and Transcriptome Sequencing
2.3. RT-PCR Validation of Differentially Expressed Genes
3. Results
3.1. Transcriptome Sequencing and Data Analysis
3.2. Differentially Expressed Genes and RT-PCR Validation
3.3. Differentially Expressed Genes Related to Plant Hormones
3.4. Differentially Expressed Genes Related to Photosynthesis and Energy Metabolism
3.5. Differentially Expressed Genes Related to Lignin and Cellulose
4. Discussion
4.1. Endogenous Hormone-Related DEGs
4.2. Lignin and Cellulose-Related DEGs
4.3. Photosynthesis and Energy Metabolism Related DEGs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | AF | DF | Transcription Factors | Description |
---|---|---|---|---|
up | ||||
Potri.005G098200 | 27.234 | 3.46619 | NAC1 | NAC(transcription factor NAC) domain containing protein 1 |
Potri.004G181900 | 545.664 | 218.295 | NAC036 | NAC domain containing protein 36 |
Potri.009G141600 | 736.5 | 296.549 | NAC036 | NAC domain containing protein 36 |
Potri.016G137900 | 3589.25 | 1505.05 | WRKY70 | WRKY(transcription factor WRKY) DNA-binding protein 70 |
Potri.006G109100 | 3168.18 | 1614.5 | WRKY70 | WRKY DNA-binding protein 70 |
Potri.002G168700 | 107.436 | 58.9119 | WRKY46 | WRKY DNA-binding protein 46 |
down | ||||
Potri.T044100 | 101.378 | 207.353 | TCP20 | TEOSINTE BRANCHED 1, TCP (transcription factor TCP)-domain family protein 20 |
Potri.011G153300 | 100.003 | 219.598 | NAC012 | NAC domain containing protein 12 |
Gene Name | AD | DD | Transcription Factors | Description | |
---|---|---|---|---|---|
up | |||||
Potri.004G211700 | 462.101 | 284.943 | ARF10 | auxin response factor 10 | |
Potri.005G126400 | 418.002 | 226.958 | BZR1 | Brassinosteroid signaling positive regulator (BZR1) family protein | |
Potri.002G133700 | 576.321 | 326.778 | BZR1 | Brassinosteroid signaling positive regulator (BZR1) family protein | |
Potri.002G142800 | 75.7639 | 42.1725 | GATA2 | GATA(transcription factor GATA) transcription factor 2 | |
Potri.009G123400 | 588.683 | 357.448 | GATA5 | GATA transcription factor 5 | |
Potri.005G116800 | 30.0181 | 4.70138 | VND1 | Vascular related NAC-domain protein 1 | |
Potri.002G168700 | 379.122 | 94.068 | WRKY46 | WRKY DNA-binding protein 46 | |
Potri.006G109100 | 3542.13 | 1256.65 | WRKY70 | WRKY DNA-binding protein 70 | |
down | |||||
Potri.003G050100 | 48.6193 | 101.898 | ATHB-15 | Homeobox-leucine zipper family protein | |
Potri.005G182900 | 76.5924 | 143.312 | AT1G21580 | Zinc finger C-x8-C-x5-C-x3-H type family protein | |
Potri.004G095100 | 2.89458 | 20.2711 | AT1G66810 | Zinc finger C-x8-C-x5-C-x3-H type family protein | |
Potri.010G118700 | 0.3343 | 22.2819 | AT1G68200 | Zinc finger C-x8-C-x5-C-x3-H type family protein | |
Potri.001G079800 | 41.3335 | 86.3043 | AT5G51190 | Integrase-type DNA-binding superfamily protein |
Gene Name | AN | DN | Transcription Factors | Description | |
---|---|---|---|---|---|
up | |||||
Potri.005G236700 | 121.671 | 50.9552 | MP | Transcriptional factor B3 family protein/auxin-responsive factor AUX/IAA-related | |
Potri.018G029500 | 182.423 | 103.379 | HY5 | Basic-leucine zipper (bZIP) transcription factor family proteisn | |
Potri.014G117000 | 25.0831 | 1.97366 | MYB2 | Myb domain protein 2 | |
Potri.002G180800 | 397.419 | 111.409 | LHY | Homeodomain-like superfamily protein | |
Potri.005G116800 | 27.7685 | 4.70297 | VND1 | Vascular related NAC-domain protein 1 | |
Potri.014G108100 | 334.082 | 153.051 | TIP | TCV-interacting protein | |
Potri.002G168700 | 294.105 | 125.42 | WRKY46 | WRKY DNA-binding protein 46 | |
down | |||||
Potri.019G112000 | 207.012 | 368.607 | AT1G05710 | Basic helix-loop-helix (bHLH) DNA-binding superfamily protein | |
Potri.008G161800 | 234.024 | 436.951 | BHLH92 | Basic helix-loop-helix (bHLH) DNA-binding superfamily protein | |
Potri.003G139300 | 180.925 | 325.849 | AT1G64380 | Integrase-type DNA-binding superfamily protein | |
Potri.001G092400 | 86.1233 | 159.747 | AT1G64380 | Integrase-type DNA-binding superfamily protein | |
Potri.007G076800 | 81.6476 | 160.502 | ABR1 | Integrase-type DNA-binding superfamily protein | |
Potri.002G142800 | 134.963 | 291.083 | GATA2 | GATA transcription factor 2 | |
Potri.004G078800 | 472.198 | 830.782 | SCL8 | SCARECROW-like 8 |
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Du, K.; Han, Q.; Zhang, Y.; Kang, X. Differential Expression of Genes Related to the Formation of Giant Leaves in Triploid Poplar. Forests 2019, 10, 920. https://doi.org/10.3390/f10100920
Du K, Han Q, Zhang Y, Kang X. Differential Expression of Genes Related to the Formation of Giant Leaves in Triploid Poplar. Forests. 2019; 10(10):920. https://doi.org/10.3390/f10100920
Chicago/Turabian StyleDu, Kang, Qiang Han, Ying Zhang, and Xiangyang Kang. 2019. "Differential Expression of Genes Related to the Formation of Giant Leaves in Triploid Poplar" Forests 10, no. 10: 920. https://doi.org/10.3390/f10100920