Next Article in Journal
Si96: A New Silicon Allotrope with Interesting Physical Properties
Previous Article in Journal
Sol-Gel Synthesis of Carbon Xerogel-ZnO Composite for Detection of Catechol
Previous Article in Special Issue
ZnO Nanostructure Templates as a Cost-Efficient Mass-Producible Route for the Development of Cellular Networks
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials
Volume 9
Issue 4
10.3390/ma9040283
materials-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells

by
Masaaki Takechi
1,
Yoshiaki Ninomiya
1,
Kouji Ohta
1,*,
Misato Tada
1,
Kazuki Sasaki
1,
Mohammad Zeshaan Rahman
1,
Akira Ohta
1,
Kanji Tsuru
2 and
Kunio Ishikawa
2
1
Department of Oral and Maxillofacial Surgery, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan
2
Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
*
Author to whom correspondence should be addressed.
Materials 2016, 9(4), 283; https://doi.org/10.3390/ma9040283
Submission received: 19 November 2015 / Revised: 3 April 2016 / Accepted: 7 April 2016 / Published: 13 April 2016
(This article belongs to the Special Issue Cellular Materials: Design and Optimisation)
Browse Figures
Versions Notes

Abstract

To improve the osteoconductivity of apatite cement (AC) for reconstruction of bone defects after oral maxillofacial surgery, we previously fabricated AC containing atelocollagen (AC(ate)). In the present study, we examined the initial attachment, proliferation and differentiation of mouse osteoblastic cells (MC3T3-E1 cells) on the surface of conventional AC (c-AC), AC(ate) and a plastic cell dish. The number of osteoblastic cells showing initial attachment to AC(ate) was greater than those attached to c-AC and similar to the number attached to the plastic cell wells. We also found that osteoblastic cells were well spread and increased their number on AC(ate) in comparison with c-AC and the wells without specimens, while the amount of procollagen type I carboxy-terminal peptide (PIPC) produced in osteoblastic cells after three days on AC(ate) was greater as compared to the others. There was no significant difference in regard to alkaline phosphatase (ALP) activity and osteocalcin production by osteoblastic cells among the three surface types after three and six days. However, after 12 days, ALP activity and the produced osteocalcin were greater with AC(ate). In conclusion, AC(ate) may be a useful material with high osteoconductivity for reconstruction of bone defects after oral maxillofacial surgery.
Keywords: apatite cement; atelocollagen; osteoconductivity; MC3T3-E1 cell proliferation apatite cement; atelocollagen; osteoconductivity; MC3T3-E1 cell proliferation

Share and Cite

MDPI and ACS Style

Takechi, M.; Ninomiya, Y.; Ohta, K.; Tada, M.; Sasaki, K.; Rahman, M.Z.; Ohta, A.; Tsuru, K.; Ishikawa, K. Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells. Materials 2016, 9, 283. https://doi.org/10.3390/ma9040283

AMA Style

Takechi M, Ninomiya Y, Ohta K, Tada M, Sasaki K, Rahman MZ, Ohta A, Tsuru K, Ishikawa K. Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells. Materials. 2016; 9(4):283. https://doi.org/10.3390/ma9040283

Chicago/Turabian Style

Takechi, Masaaki, Yoshiaki Ninomiya, Kouji Ohta, Misato Tada, Kazuki Sasaki, Mohammad Zeshaan Rahman, Akira Ohta, Kanji Tsuru, and Kunio Ishikawa. 2016. "Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells" Materials 9, no. 4: 283. https://doi.org/10.3390/ma9040283

APA Style

Takechi, M., Ninomiya, Y., Ohta, K., Tada, M., Sasaki, K., Rahman, M. Z., Ohta, A., Tsuru, K., & Ishikawa, K. (2016). Effects of Apatite Cement Containing Atelocollagen on Attachment to and Proliferation and Differentiation of MC3T3-E1 Osteoblastic Cells. Materials, 9(4), 283. https://doi.org/10.3390/ma9040283

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop