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Open AccessFeature PaperArticle

Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites

Unité Mixte de Recherche (UMR) S949, Inserm, Strasbourg 67000, France
Etablissement Français du Sang-Alsace (EFS-Alsace), Strasbourg 67000, France
Fédération de Médecine Translationnelle de Strasbourg (FMTS), Strasbourg 67000, France
Université de Strasbourg, Strasbourg 67000, France
Materials Engineering Center, UdR INSTM, University of Perugia, Terni 05100, Italy
Department of Molecular Medicine, INSTM UdR of Pavia, Biochemistry Unit, “A Castellani”, Viale Taramelli, 3/b-27100 Pavia, Center for Health Technologies (C.H.T.), University of Pavia, Pavia 27100, Italy
Department of Public Health, Experimental Medicine and Forensics, University of Pavia, Pavia 27100, Italy
Department of Occupational Medicine, Toxicology and Environmental Risks, S. Maugeri Foundation, IRCCS Pavia 27100, Italy
Research Unit on Implant Infections, Rizzoli Orthopaedic Institute, and DIMES, University of Bologna, Via di Barbiano 1/10-40136, Bologna, Italy
Authors to whom correspondence should be addressed.
Academic Editor: Nadia Jessel
Materials 2016, 9(1), 37;
Received: 19 November 2015 / Revised: 20 December 2015 / Accepted: 21 December 2015 / Published: 11 January 2016
(This article belongs to the Special Issue Anti-Infective Materials in Medicine and Technology)
The purpose of this study was to investigate the antimicrobial properties of multifunctional nanocomposites based on poly(dl-Lactide-co-Glycolide) (PLGA) and increasing concentration of silver (Ag) nanoparticles and their effects on cell viability for biomedical applications. PLGA nanocomposite films, produced by solvent casting with 1 wt%, 3 wt% and 7 wt% of Ag nanoparticles were investigated and surface properties were characterized by atomic force microscopy and contact angle measurements. Antibacterial tests were performed using an Escherichia coli RB and Staphylococcus aureus 8325-4 strains. The cell viability and morphology were performed with a murine fibroblast cell line (L929) and a human osteosarcoma cell line (SAOS-2) by cell viability assay and electron microscopy observations. Matrix protein secretion and deposition were also quantified by enzyme-linked immunosorbent assay (ELISA). The results suggest that the PLGA film morphology can be modified introducing a small percentage of silver nanoparticles, which induce the onset of porous round-like microstructures and also affect the wettability. The PLGA/Ag films having silver nanoparticles of more than 3 wt% showed antibacterial effects against E. coli and S. aureus. Furthermore, silver-containing PLGA films displayed also a good cytocompatibility when assayed with L929 and SAOS-2 cells; indicating the PLGA/3Ag nanocomposite film as a promising candidate for tissue engineering applications. View Full-Text
Keywords: Ag nanocomposites; cell cytocompatibility; antibacterial activity Ag nanocomposites; cell cytocompatibility; antibacterial activity
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Scavone, M.; Armentano, I.; Fortunati, E.; Cristofaro, F.; Mattioli, S.; Torre, L.; Kenny, J.M.; Imbriani, M.; Arciola, C.R.; Visai, L. Antimicrobial Properties and Cytocompatibility of PLGA/Ag Nanocomposites. Materials 2016, 9, 37.

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