Next Article in Journal
Structural Analysis of Lignin-Based Furan Resin
Next Article in Special Issue
Structural Characterization of Multicomponent Crystals Formed from Diclofenac and Acridines
Previous Article in Journal
Investigations on Flexural and Compressive Strengths of Mortar Dedicated to Clinker Units—Influence of Mixing Water Content and Curing Time
Previous Article in Special Issue
Single-Crystal X-ray and Solid-State NMR Characterisation of AND-1184 and Its Hydrochloride Form
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Materials
Volume 15
Issue 1
10.3390/ma15010349
materials-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Relationship between the Crystal Structure and Tuberculostatic Activity of Some 2-Amidinothiosemicarbazone Derivatives of Pyridine

by
Katarzyna Gobis
1,*,
Małgorzata Szczesio
2,
Andrzej Olczak
2,
Tomasz Pawlak
3,
Ewa Augustynowicz-Kopeć
4,
Malwina Krause
1 and
Marek L. Główka
2
1
Department of Organic Chemistry, Medical University of Gdańsk, 107 Gen. Hallera Av., 80-438 Gdansk, Poland
2
Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland
3
Centre of Molecular and Macromolecular Studies, Polish Academy of Science, 112 Sienkiewicza, 90-363 Lodz, Poland
4
Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 26 Płocka Str., 01-138 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Materials 2022, 15(1), 349; https://doi.org/10.3390/ma15010349
Submission received: 10 December 2021 / Revised: 27 December 2021 / Accepted: 31 December 2021 / Published: 4 January 2022
(This article belongs to the Special Issue Crystal Growth and Structure)
Browse Figures
Review Reports Versions Notes

Abstract

:
Tuberculosis remains one of the most common diseases affecting developing countries due to difficult living conditions, the rapidly increasing resistance of M. tuberculosis strains and the small number of effective anti-tuberculosis drugs. This study concerns the relationship between molecular structure observed in a solid-state by X-ray diffraction and the 15N NMR of a group of pyridine derivatives, from which promising activity against M. tuberculosis was reported earlier. It was found that the compounds exist in two tautomeric forms: neutral and zwitterionic. The latter form forced the molecules to adopt a stable, unique, flat frame due to conjugation and the intramolecular hydrogen bond system. As the compounds exist in a zwitterionic form in the crystal state generally showing higher activity against tuberculosis, it may indicate that this geometry of molecules is the “active” form.

1. Introduction

The widespread use of antibiotics in intensive livestock farming, the subsequent penetration of drugs and their metabolites into groundwater, and excessively and hastily prescribing antibiotics resulted in the emergence of many bacterial strains characterized by drug resistance [1]. Increasing drug resistance among species of pathogenic bacteria poses a significant problem for the treatment of infectious diseases. One such disease is tuberculosis, caused by Mycobacterium tuberculosis [2]. According to the World Health Organization, 10 million people fell ill with tuberculosis in 2019 (including 1.2 million children under the age of 14), of which 1.4 million died. Five hundred thousand cases were resistant to rifampicin—one of the first-line drugs—and 78% were resistant to other tuberculostatics. Unfortunately, only one in three patients entered treatment [3].
One of the main reasons for the continued spread of TB (tuberculosis) is the narrow range of effective drugs. Their number remains limited due to difficulties associated with searching for new and active substances, despite several findings in structure–activity relationship studies. The difficulties in efficient implementation are the slow replication of mycobacteria, that M. tuberculosis belongs to the third group of pathogens according to the biosafety level (BSL), the shortage of scientific centers authorized to conduct research on M. tuberculosis strains, difficulties in mapping the specific environment inside the host of mycobacteria in animal models, the not fully understood biology of mycobacteria in latent form, and the hindered penetration of chemotherapeutic agents through the cell wall [4,5,6].
The study described below is a continuation of our previous studies on amidrazone derivatives exhibiting high anti-tuberculosis activity [7,8,9,10,11,12]. The previous studies allowed us to formulate a hypothesis that the planarity of these molecules being isoniazid analogues (INH) is a significant prerequisite for their tuberculostatic activity. Among others, S-methyl and S,S-dimethylamino(pyridin-2-ylmethylene)carbono-hydrazonodithioates were synthesized. These compounds showed high tuberculostatic activity in vitro with a minimum inhibitory concentration (MIC ) value of 3.13 µg/mL. Crystallographic studies were carried out for a flat structure due to conjugation and the formation of intramolecular hydrogen bonds (Figure 1) [12,13].
The same conditions are also met by some 4-substituted picolinonitrile derivatives obtained in the course of this study, having an amidrazone function in the C-2 position of the pyridine ring (Figure 2). For some of them, we obtained very promising activity against M. tuberculosis strains with MIC values of 0.4 µg/mL [14]. In this research, we try to explain the relationship between their activity and the structural features of their molecules. For example, an interesting property of the tested amidrazone derivatives is their presence in two tautomeric forms, which results in different conformations and different intramolecular hydrogen bonds. As the molecular mechanism of tuberculostatic activity for the studied compounds is unknown, we tried to find any relationship among more active compounds; however, their number is limited.

2. Materials and Methods

2.1. Synthesis

All materials and solvents were of analytical reagent grade (Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). Thin-layer chromatography was performed on Merck silica gel 60F254 plates and visualized with UV light. The results of elemental analyses (%C, H, N) for all of the obtained compounds were in agreement with the calculated values that were within the ± 0.4%range. Standard 1H and 13C NMR spectra for 4-phenylthiopicolinonitrile and 4-phenylthiopicolinohydrazonamide in DMSO-d6 were recorded on a Varian Unity Plus instrument (Palo Alto, CA). IR spectra (KBr) were determined as KBr pellets of the solids on a Satellite FT-IR spectrophotometer (Madison, WI). Melting points were determined via a Stuart SMP30 apparatus and were retained without any corrections.

2.1.1. 4-Phenylthiopicolinonitrile

First, 5.60 g (40 mmol) of 4-chloropicolinonitrile and 4.91 mL (48 mmol) of thiophenol were dissolved in 25 mL of dioxane, and then 6 mL (40 mmol) of DBU (1,8-diazabicyclo [5.4.0]undec-7-ene) were added. The mixture was refluxed for 1 h. Next, some of the solvent was evaporated and ice was added. The resulting precipitate was filtered off and dried. The crude product was purified by recrystallization from cyclohexane. Yield: 98%; mp 51–53 °C; IR (KBr): 3047 (υ CAr-H); 2234 (υ C ≡ N); 1647 (υ C = N); 1570, 1437 (υ C = C); 835, 759, 691 (γ C–H) cm−1; 1H NMR (500 MHz, DMSO-d6): δ 7.19 (dd, 1H, pyridine, J1 = 2 Hz, J2 = 3 Hz); 7.54-7.63 (m, 5H, ArH); 7.68 (d, 1H, pyridine, J = 2Hz); 8.47 (d, 1H, pyridine, J = 5Hz) ppm; Elemental analysis for C12H8N2S (212.04) calculated: C, 67.90; H, 3.80; N, 13.20; found: C, 67.73; H, 3.79; N, 13.02.

2.1.2. 4-Phenylthiopicolinohydrazonamide

To a solution of 2.12 g (10 mmol) of 4-phenylthiopicolinonitrile in 75 mL of methanol, 2 mL (13.5 mmol) of DBU was added. The mixture was refluxed for 4 h. Next, some of the solvent was evaporated, and 1 mL (31.7 mmol) of an 80% hydrazine hydrate solution was added. The solution was refluxed for 15 min. The mixture was then cooled, and 5 mL of diethyl ether was added. The precipitate was filtered off, dried and recrystallized from methanol. Yield: 65%; mp 101–103 °C; IR (KBr): 3445, 3351 (υ N-H); 3072, 3051 (υ CArH); 1574 (δ N-H); 1469, 1439 (υ C = C); 750, 689 (γ C-H) cm−1; 1H NMR (500 MHz, DMSO-d6): δ 6.46 (br s, 2H, NH2); 7.17 (dd, 1H, pyridine, J1 = 2Hz, J2 = 3Hz); 7.50–7.58 (m, 4H, 2ArH + NH2); 7.61–7.63 (m, 3H, ArH); 7.73 (d, 1H, pyridine, J = 2Hz); 8.40 (d, 1H, pyridine, J = 5Hz) ppm; 13C NMR (175 MHz, DMSO-d6): δ 117.27; 121.94; 128.75; 130.57; 130.75 (2C); 130.89; 135.59; 135.66 (2C); 148.72; 150.53 ppm; Elemental analysis for C12H12N4S (244.08) calculated: C, 58.99; H, 4.95; N, 22.93; found: C, 59.04; H, 4.93; N, 22.81.

2.1.3. 2-[Amino-(4-phenylthiopyridin-2-yl)methylene]-N-cyclohexylhydrazinecarbothioamide (1)

First, 0.244 g (1 mmol) of 4-phenylthiopicolinohydrazonamide was dissolved in 5 mL of dioxane, and then 0.155 mL (1.1 mmol) of cyclohexyl isothiocyanate was added. The mixture was refluxed for 5 min. Next, the mixture was cooled, and diethyl ether was added. The product was filtered off, dried and recrystallized from ethanol. Yield: 58%; mp 200–202 °C; IR (KBr): 3398, 3343, 3172 (υ N–H); 2929, 2851 (υ C–H); 1658 (υ C = N); 1573, 1527 (δ N–H); 1220 (υ C–N); 750 (γ Car–H) cm−1; 1H NMR (500 MHz, DMSO-d6): δ 1.17–1.29 (m, 5H, CH); 1.61–1.84 (m, 5H, CH); 4,05 (s, 1H, CH); 6.91 (s, 2H, NH2); 7.11 (dd, 1H, pyridine, J1 =2 Hz, J2=3 Hz); 7.26 (d, 1H, ArH, J = 9 Hz); 7.54–7.55 (m, 3H, ArH); 7.62–7.64 (m, 2H, ArH +NH); 7.71 (s, 1H, pyridine); 8.35 (d, 1H, pyridine, J = 5 Hz); 9.97 (s, 1H, NH) ppm; Elemental analysis for C19H23N5S2 (385.14) calculated: C, 59.19; H, 6.01; N, 18.16; found: C, 58.82; H, 5.75; N, 17.87.

2.2. Tuberculostatic Activity

Compounds 1–6 were tested using the classic test tube method consisting of successive dilution in the Proskauer and Beck modification on Youmans liquid medium with the addition of 10% bovine serum [15,16]. Bacterial suspensions were prepared from 14-day-old cultures of slow-growing strains and 48-h cultures of saprophytic strains [17]. The obtained compounds were dissolved in ethylene glycol at a concentration of 10 mg mL. Next, exponential dilutions were made in Youmans medium. Incubations were carried out at 37 °C for 21 days. The minimum inhibitory concentration (MIC) values were defined as the minimum concentration that inhibits the growth of the tuberculosis strains tested. As a control, a medium containing no test compounds was used. Isoniazid (INH) and pyrazinamide (PZA) were used as reference drugs.

2.3. X-ray

The crystal measurements of compounds 1, 2, 5 and 6 were performed on an XtaLAB Synergy diffractometer, Dualflex, Pilatus 300K (Rigaku Corporation, Tokyo, Japan), while measurements of compounds 3 and 4 were performed on Bruker SMART APEXII CCD Diffractometer (Bruker AXS Inc., Madison, WI, USA). All diffraction experiments were carried out with CuKα radiation. For data processing of compounds 1, 2, 5 and 6, CrysAlis PRO (Rigaku Oxford Diffraction Ltd., Yarnton, Oxfordshire, UK, 2020) was used. The diffraction data of compounds 3 and 4 were processed with SAINT ver. 8.34A, SADABS ver. 2014/4 and XPREP ver. 2014/2 (Bruker AXS Inc., Madison, WI, USA). The structures were determined with the ShelXT 2018/2 solution program (Version 2018/2, 2018, Göttingen, Germany) [18] and refined with ShelXL 2018/3 [19] using full matrix least-squares minimization on F2. For visualization, ShelXle [20] was used. All H atoms (except those engaged in hydrogen bonds) were geometrically optimized and allowed as riding atoms, with the appropriate C—H distances for the respective temperatures of the diffraction experiments and with Uiso(H) = 1.2 Ueq(C, N). All studied crystals were obtained by the slow evaporation of the solvent, a mixture of methanol and DMF (1:1). The tables and figures were prepared using the publCIF [21] and Mercury [22] programs.
CCDC 2125507, 2125509, 2125514, 2125531, 2125534, 2125535 contain the supplementary crystallographic data for this paper. The data is provided free of charge by The Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/structures.

2.4. Solution NMR

All NMR experiments were run at 298 K on a 600 MHz Bruker Avance III spectrometer, equipped with an HCN probe head operating at 600.15 and 60.81 MHz for 1H and 15N nuclei, respectively. The samples were prepared in DMSO-d6 (99.8% + D) from Armar Chemicals. The 13N NMR data were assigned using standard 2D 1H-15N NMR correlation techniques and gradient-selected heteronuclear single-quantum correlation (gs-HSQC) [23].

2.5. Solid-State NMR

Solid-state cross-polarization magic angle spinning (CP/MAS) NMR experiments were performed on a 400 MHz Avance III spectrometer (operating at 400.13 MHz, 40.55 MHz for 1H and 15N) equipped with a MAS probe head using 4-mm ZrO2 rotors. A sample of 13C, 15N-labeled histidine hydrochloride was used to set the Hartmann–Hahn condition and the secondary reference sample for 15N. The 15N CP/MAS spectra were performed with a proton 90° pulse length of 4 μs, a contact time of 2 ms, a repetition delay of 25 s and 5 s for compounds 1 and 4, respectively. A spectral width of 25 kHz and a time-domain size of 32 k data points were applied. The acquisition data were collected with a SPINAL decoupling sequence [24]. Spectra were processed on a PC using the Bruker TopSpin 3.6 program [25].

2.6. QM Calculations

The DFT calculations were performed using the CASTEP 19.11 code [26]. The geometry optimization of all atomic positions and unit cell parameters were performed, starting with the X-ray diffraction crystal structures of compounds 1 and 4. The PBE (Perdew–Burke-Ernzerhof) functional with the TS (Tkatchenko-Scheffler) dispersion correction scheme (DFT-D method) was applied [27,28,29]. A comparison of the average forces remaining on the atoms after geometry optimization was carried out using a maximum plane-wave cutoff energy of 800 eV and ultrasoft pseudopotential [30]. A Monkhorst-Pack grid was used to sample the Brillouin zone [31]. The NMR chemical shifts were computed using the gauge, including the projected augmented wave (GIPAW) method [32,33]. In all cases, the optimization algorithm was Broyden–Fletcher–Shanno-Goldfarb (BFSG ) with a line search [34].

3. Results and Discussion

3.1. Synthesis

The new derivative 2-[amino-(4-phenylthiopyridin-2-yl)methylene]-N-cyclohexylhydrazinecarbothioamide 1 was obtained according to the presented Scheme 1. The substrate for the synthesis was commercially available 4-chloropicolinonitrile (Apollo Scientific, Stockport, UK). In the first step, the chlorine atom in the C4 position was subjected to a thiophenyl substitution reaction. The reaction was carried out in dioxane with the addition of 1,8-diazabicyclo [5.4.0]undec-7-ene (DBU). To prepare the hydrazonamide derivative of 4-phenylthiopicolinonitrile, an 80% solution of hydrazine hydrate was added to the methyliminoester solution prepared in situ. A method developed at the Department of Organic Chemistry, Medical University of Gdańsk was used to obtain the methylimino ester, which consisted of carrying out the reaction in a methanol environment and DBU. The reaction was carried out for 4 h in reflux. The obtained 4-phenylthiopicolinohydrazonamide was reacted with cyclohexyl isothiocyanate according to the method described by Brown et al. [35]. The yields of the performed reactions were in the range of 58–98%. The synthesis of compounds 2–6 was described by the authors earlier [14].

3.2. Tuberculostatic Activity

The obtained compounds were tested in vitro for their tuberculostatic activity. The study was carried out on two strains of M. tuberculosis: standard H37Rv and a clinical strain isolated from patients of Spec. 210, which showed resistance to isoniazid, rifampicin, ethambutol and p-aminosalicylic acid. Their anti-mycobacterial activity was expressed by the minimum inhibitory concentration (MIC) defined as the minimum concentration inhibiting the growth of the tested microorganisms. Significant discrepancies in the MIC and its low value in all tested compounds prompted us to study their structure in more detail.
All compounds tested, except compounds 1 and 6, showed moderate antitubercular activity with MIC values ranging from 6.2–12.5 µg/mL [14]. Compound 1, possessing a cyclohexyl ring at a terminal N atom and a hydrogen atom at this nitrogen, showed the lowest activity (MIC 25–50 µg/mL). Moreover, this compound showed two times lower activity against the resistant strain, while all other compounds presented in Table 1 did not show different activity against the resistant and the standard strains. As only 1 appeared in neutral form, while all other compounds studied here (having higher activity) were detected as zwitterions in the solid-state, one may suggest that the latter form is “an active one”. Among the studied species existing in zwitterionic form, compound 6 showed the highest activity with a MIC of only 0.4 µg/mL, most probably due to a good fit with an unknown receptor. Moreover, the compounds demonstrated the same antitubercular activity against the standard H37Rv as against the resistant Spec. 210 strain. Compared with the values ascribed to isoniazid (MIC of 3.1 and 12.5 µg/mL), the values for 6 are almost eight times less for the standard strain and as much as 31 times less (6 being more active), respectively, for the resistant bacteria.

3.3. Crystallography

The crystal structures of six compounds were determined (Table 2, Figure 3). Only compound 1 adopts the neutral form, while the others form zwitterions in the solid-state.
The structure of compound 1 is the only one in which molecules form dimers through strong hydrogen bonds of the N—H···N type (Table 3, Figure 4). These dimers form ribbons by hydrogen bonds of the N—H···S type. The conformation of the molecule is stabilized by intramolecular hydrogen bonds (N4—H4···N42). The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.1586 and 0.0762 Å). Interestingly, DFT calculations of energies (for 6-311 G(d,p) standard basis set and taking into account the solvent effect of water using the polarizable continuum model) for the optimized geometries of molecules 1 and 4 revealed that the forms present in the crystals are more stable than the corresponding tautomeric forms, e.g., the energy of the neutral form of 1 has an energy lower by about 8 kcal/mol, and the energy of both molecules of 4 is lower in the zwitterionic form by approximately 4 kcal/mol.
Contrary to compound 1, in a crystal state, the molecules of all other compounds studied here adopt the form of zwitterions with hydrogen found at the N3 atom. The pyridine ring in these structures is in a reversed position and forms characteristic bifurcated intramolecular hydrogen bonds N(42)···H(N3)···S1 (Table 4, Table 5, Table 6, Table 7 and Table 8). Additionally, rotation along the N2-C1 bond occurs.
Structure 2 contains two molecules in the independent part of the unit cell. The molecules form infinite chains through N-H···S hydrogen bonds (Table 4, Figure 5). The conformation of the molecules is stabilized by intramolecular hydrogen bonds of the N-H···N type. The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.0421 and 0.0846 Å).
In its crystal, the molecules of compound 3 form a chain via N4—HbB···S1 type hydrogen bonds (Figure 6, Table 5). Additionally, intramolecular hydrogen bonds are formed (N4—H4A···N2). The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.0847 Å).
In the structure of compound 4, there are two molecules of the compound, two molecules of water and two molecules of DMF (one disordered), in the independent part of the unit cell. The molecules have different positions of substituents in the piperazine ring (equatorial–equatorial or equatorial–axial), with the phenyl always in the equatorial position. This compound also forms chains of hydrogen-bonded molecules with the water molecules as “bridges” (Table 6, Figure 7). The DMF molecules fill the gaps and form hydrogen bonds of the N-H···O type (Figure 8). The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.0655 and 0.0830 Å).
The molecules of 5 form chains via N3-H3···S1 hydrogen bonds (Table 7, Figure 9) in the studied crystal. Additionally, intramolecular hydrogen bonds (N4-H4B···S1) are formed. The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.0775 Å).
The crystal structure of compound 6 reveals the standard N-H···S hydrogen bonds as the rest of the zwitterion compounds studied here, forming infinite chains (Table 8, Figure 10). Additionally, the molecules form intermolecular hydrogen bonds with oxygen atoms in morpholine rings. The molecule fragment (N’-thioformylpicolinohydrazonamide) takes a flat form (r.m.s. deviation = 0.0871 Å).
The molecules of compound 6 in the zwitterion form have an extended conformation, while compound 1 (neutral form) has a bent form (Figure 11). The pattern of intramolecular hydrogen bonds depends on the form adopted.
There are 16 analogous crystal structures in the CSD [36,37] database: eight are in the zwitterionic form, and eight are in the neutral one (Figure 12). Several of them originated from our laboratories, and their antitubercular activity is known. Unfortunately, other studies on the crystal structure of similar compounds do not mention their activity, except that of Almeida et al. [38], which quotes the lowest MIC of about 160 [µg/mL] for antitubercular action, well above the values found in the studied species here (Table 1). All compounds in each of the two groups have the same intramolecular pattern of hydrogen bonds related to the form in which the molecules occur, which is shown (Figure 12 and Figure 13) by overlaying an N(H2)-C-N-N-C(S) fragment in all 16 structures. Only compounds in zwitterionic form adopt an elongated geometry.

3.4. NMR

The 15N CP MAS spectra of compound 1 (a) and compound 4 (b) are shown in Figure 14. As described in the previous section, both structures contain two crystallographically nonequivalent molecules in the asymmetric part of the unit cell. It is also easily visible for the compound 1 spectrum, where most of the positions show separated resonance lines. However, even a brief look at the compound 4 spectrum shows that most of the positions are isochronous and overlap, giving single resonances. The full assignment of 15N CP MAS signals to the conformers is not an easy task. To solve this problem, we applied the GIPAW method. The usefulness of this approach for the assignment of solid-state NMR signals is unquestionable [48,49,50]. After comparing the experimentally obtained chemical shifts with GIPAW calculation nuclear shieldings, we were able to assign 15N CP MAS signals, as shown in Figure 14. The numerical values are presented in Table 9 and Table 10.
The main question regarding these two structures was the localisation of the hydrogen atom in the -N = NH- link. These positions are indicated as N2 and N3 in the crystal structures for both structures. Keeping in mind the uncertain location of the hydrogen atom we investigated, two theoretical models with the different locations of the hydrogen atom were constructed, and GIPAW calculations were performed. Our results, presented in Table 9 and Table 10, fully supported the supposition that in a solid-state, the hydrogen atom is localised in the same position where it was found in single crystal X-ray structures. However, it has to be stressed that such a statement is not fully unambiguous for compound 1 since the differences between both tautomers are rather small. Nevertheless, it means that the selected single crystal for X-ray measurement is representative of the bulk material. Our additional 15N NMR measurements in a DMSO-d6 solution provided the same conclusion about the studied material. The 15N NMR spectra clearly show only one cross peak in Figure 15, which suggests that there is only one tautomeric form, also in the liquid state. The chemical shifts recorded for the –NH = signal are very similar to the solid-state NMR values, which suggests that the same tautomeric form is in the solid-state and the DMSO-d6 solution.

4. Conclusions

The structures of six new 2-amidinothiosemicarbazone derivatives of pyridine were determined in the solid-state by means of X-ray diffraction and NMR. For the first time, synthesis and tuberculostatic activity are also presented for compound 1. The studied amidrazone derivatives exist either in zwitterionic or in neutral form. Zwitterionic molecules in the solid-state adopt an extended conformation due to conjugation and a unique intramolecular hydrogen-bonds pattern, which secures both the planarity and similarity of the common frame of these molecules. Protonated nitrogen at the C = S group, i.e., having other properties, may influence the preferred form of the molecule. In general, the compounds that prefer the zwitterionic state in their crystals show higher antitubercular activity than the compound detected in the neutral form.

Author Contributions

Conceptualization, K.G., M.S., A.O., M.L.G. and T.P.; synthesis, K.G. and M.K.; tuberculostatic activity, E.A.-K.; methodology, K.G., M.S., A.O., T.P. and E.A.-K.; software, M.S., A.O. and T.P.; validation, M.S., A.O., M.L.G. and T.P.; writing—original draft preparation, K.G., M.S., A.O., M.L.G. and T.P.; writing—review and editing, K.G., M.S., A.O., M.L.G. and T.P.; visualization, M.S., A.O. and T.P.; fundraising for research, K.G. All authors have read and agreed to the published version of the manuscript.

Funding

This project was funded by the National Science Centre (Cracow, Poland) based on decision number 2017/25/B/NZ7/00124.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are contained within the article.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Fair, R.J.; Tor, Y. Antibiotics and Bacterial Resistance in the 21st Century. Perspect. Med. Chem. 2014, 6, 25–64. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Koul, A.; Arnoult, E.; Lounis, N.; Guillemont, J.; Andries, K. The challenge of new drug discovery for tuberculosis. Nature 2011, 469, 483–490. [Google Scholar] [CrossRef]
  3. Global Tuberculosis Report. Available online: https://www.who.int/teams/global-tuberculosis-programme/tb-reports/global-tuberculosis-report-2021 (accessed on 14 October 2021).
  4. Bhat, Z.S.; Rather, M.A.; Maqbool, M.; Ahmad, Z. Drug targets exploited in Mycobacterium tuberculosis: Pitfalls and promises on the horizon. Biomed. Pharmacother. 2018, 103, 1733–1747. [Google Scholar] [CrossRef] [PubMed]
  5. Liu, Y.; Matsumoto, M.; Ishida, H.; Ohguro, K.; Yoshitake, M.; Gupta, R.; Geiter, L.; Hafkin, J. Delamanid: From discovery to its use for pulmonary multidrug-resistant tuberculosis (MDR-TB). Tuberculosis 2018, 111, 20–30. [Google Scholar] [CrossRef]
  6. Rather, M.A.; Maqbool, M. Cell wall: A versatile fountain of drug targets in Mycobacterium tuberculosis. Biomed. Pharmacother. 2017, 95, 1520–1534. [Google Scholar]
  7. Szczesio, M.; Gołka, J.; Korona-Głowniak, I.; Orlewska, C.; Gobis, K.; Olczak, A. Planarity of heteroaryldithiocarbazic acid derivatives showing tuberculostatic activity: Structure–activity relationships. Acta Cryst. 2018, C74, 400–405. [Google Scholar] [CrossRef] [PubMed]
  8. Krause, M.; Foks, H.; Augustynowicz-Kopeć, E.; Napiórkowska, A.; Szczesio, M.; Gobis, K. Synthesis and Tuberculostatic Activity Evaluation of Novel Benzazoles with Alkyl, Cycloalkyl or Pyridine Moiety. Molecules 2018, 23, 985. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  9. Szczesio, M.; Korona-Głowniak, I.; Gobis, K. Planarity of benzoyldithiocarbazate tuberculostatics. V antibacterial activities of diesters of benzoyldithiocarbazic acid. J. Mol. Struct. 2018, 1167, 127–133. [Google Scholar] [CrossRef]
  10. Olczak, A.; Szczesio, M.; Gołka, J.; Orlewska, C.; Gobis, K.; Foks, H.; Główka, M.L. Planarity of heteroaryldithiocarbazic acid derivatives showing tuberculostatic activity. II Crystal structures of 3-[amino(pyrazin-2-yl)methylidene]-2-methylcarbazic acid esters. Acta Cryst. 2011, C67, o37–o42. [Google Scholar] [CrossRef] [PubMed]
  11. Olczak, A.; Główka, M.L.; Gołka, J.; Szczesio, M.; Bojarska, J.; Kozłowska, K.; Foks, H.; Orlewska, C. Is planarity of pyridin-2-yl- and pyrazin-2-yl-formamide thiosemicarbazones related to their tuberculostatic activity? X-ray structures of two pyrazine-2-carboxamide-N’-carbonothioyl- hydrazones. J. Mol. Struct. 2007, 830, 171–175. [Google Scholar] [CrossRef]
  12. Główka, M.L.; Martynowski, D.; Olczak, A.; Orlewska, C.; Foks, H.; Bojarska, J.; Szczesio, M.; Gołka, J. Planarity of N’-(amino-2-pyridylmethylene)-hydrazide carbodithioic acid frame and crystal structure of its methyl ester dihydrate. J. Chem. Crystallogr. 2005, 35, 477–480. [Google Scholar] [CrossRef]
  13. Szczesio, M.; Gobis, K.; Korona-Głowniak, I.; Ziembicka, D.; Olczak, A. Structure and antibacterial activity of selected hydrazide derivatives. Z. Kristall. Suppl. 2020, 40, abs. P60, 85. [Google Scholar]
  14. Krause, M.; Foks, H.; Ziembicka, D.; Augustynowicz-Kopeć, E.; Głogowska, A.; Korona-Głowniak, I.; Bojanowski, K.; Siluk, D.; Gobis, K. 4-Substituted picolinohydrazonamides as a new class of potential antitubercular agents. Eur. J. Med. Chem. 2020, 190, 112106. [Google Scholar] [CrossRef] [PubMed]
  15. Youmans, G.P.; Youmans, A.S. A method for the determination of the rate of growth of tubercle bacilli by the use of small inocula. J. Bacteriol. 1949, 58, 247–255. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Youmans, G.P. Test tube evaluation of tuberculostatic agents. Am. Rev. Tuberc. 1947, 56, 376. [Google Scholar]
  17. Atlas, R.M.; Snyder, J.W. Handbook of Media for Clinical Microbiology, 2nd ed.; CRC Press: Boca Raton, MA, USA, 2006. [Google Scholar]
  18. Sheldrick, G.M. SHELXT—Integrated space-group and crystal-structure determination. Acta Cryst. 2015, C71, 3–8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Sheldrick, G.M. A short history of SHELX. Acta Cryst. 2008, A64, 112–122. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  20. Hübschle, C.B.; Sheldrick, G.M.; Dittrich, B. ShelXle: A Qt graphical user interface for SHELXL. J. Appl. Cryst. 2011, 44, 1281–1284. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  21. Westrip, S.P. publCIF: Software for editing, validating and formatting crystallographic information files. J. Appl. Cryst. 2010, 43, 920–925. [Google Scholar] [CrossRef] [Green Version]
  22. Macrae, C.F.; Bruno, I.J.; Chisholm, J.A.; Edgington, P.R.; McCabe, P.; Pidcock, E.; Rodriguez-Monge, L.; Taylor, R.; van de Streek, J.; Wood, P.A. Mercury CSD 2.0—New features for the visualization and investigation of crystal structures. J. Appl. Cryst. 2008, 41, 466–470. [Google Scholar] [CrossRef]
  23. Bodenhausen, G.; Ruben, D.J. Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy. Chem. Phys. Lett. 1980, 69, 185–189. [Google Scholar] [CrossRef] [Green Version]
  24. Fung, B.M.; Khitrin, A.K.; Ermolaev, K. An Improved Broadband Decoupling Sequence for Liquid Crystals and Solids. J. Magn. Reson. 2000, 142, 97–101. [Google Scholar] [CrossRef] [PubMed]
  25. Topspin; Version 3.6; Bruker Biospin Gmbh: Karlsruhe, Germany, 2020.
  26. Clark, S.J.; Segall, M.D.; Pickard, C.J.; Hasnip, P.J.; Probert, M.J.; Refson, K.; Payne, M.C. First Principles Methods Using CASTEP. Z. Krist. 2005, 220, 567–570. [Google Scholar] [CrossRef] [Green Version]
  27. Perdew, J.P.; Burke, K.; Ernzerhof, K.M. Generalized Gradient Approximation Made Simple. Phys. Rev. Lett. 1996, 77, 3865–3868. [Google Scholar] [CrossRef] [Green Version]
  28. McNellis, E.R.; Meyer, J.; Reuter, K. Azobenzene at Coinage Metal Surfaces: Role of Dispersive van Der Waals Interactions. Phys. Rev. B 2009, 80, 205414. [Google Scholar] [CrossRef] [Green Version]
  29. Tkatchenko, A.; Scheffler, M. Accurate Molecular Van Der Waals Interactions from Ground-State Electron Density and Free-Atom Reference Data. Phys. Rev. Lett. 2009, 102, 073005. [Google Scholar] [CrossRef] [Green Version]
  30. Vanderbilt, D. Soft Self-Consistent Pseudopotentials in a Generalized Eigenvalue Formalism. Phys. Rev. B 1990, 41, 7892–7895. [Google Scholar] [CrossRef] [PubMed]
  31. Monkhorst, H.J.; Pack, J.D. On Special Points for Brillouin Zone Integrations. Phys. Rev. B 1976, 13, 5188–5912. [Google Scholar] [CrossRef]
  32. Pickard, C.J.; Mauri, F. All-Electron Magnetic Response with Pseudopotentials: NMR Chemical Shifts. Phys. Rev. B 2001, 63, 245101. [Google Scholar] [CrossRef] [Green Version]
  33. Yates, J.R.; Pickard, C.J.; Mauri, F. Calculation of NMR Chemical Shifts for Extended Systems Using Ultrasoft Pseudopotentials. Phys. Rev. B 2007, 76, 024401. [Google Scholar] [CrossRef]
  34. Nocedal, J.; Wright, S.J. Numerical Optimization, 2nd ed.; Springer: Berlin, Germany; New York, NY, USA, 2006. [Google Scholar]
  35. Brown, K.K.; Hampton, M.B. Bological targets of isothiocyanates. Biochim. Biophys. Acta 2011, 1810, 888–894. [Google Scholar] [CrossRef] [PubMed]
  36. Groom, C.R.; Bruno, I.J.; Lightfoot, M.P.; Ward, S.C. The Cambridge Structural Database. Acta Cryst. 2016, B72, 171–179. [Google Scholar] [CrossRef] [PubMed]
  37. Bruno, I.J.; Cole, J.C.; Edgington, P.R.; Kessler, M.; Macrae, C.F.; McCabe, P.; Pearson, J.; Taylor, R. New software for searching the Cambridge Structural Database and visualising crystal structures. Acta Cryst. 2002, B58, 389–397. [Google Scholar] [CrossRef]
  38. Almeida, J.C.L.; Amin, R.S.; Pessoa, C.; Laurenco, M.C.S.; Mendes, L.C.; Lessa, J.A. Bismuth(III) complexes with pyrazineformamide thiosemicarbazones: Investigation on the antimicrobial and cytotoxic effects. Polyhedron 2020, 189, 114709–114715. [Google Scholar] [CrossRef]
  39. Bermejo, E.; Castineiras, A.; Garcıa-Santos, I.; West, D.X. Structural and Coordinative Variability in Zinc(II), Cadmium(II), and Mercury(II) Complexes of 2-Pyridineformamide 3-Hexamethyleneiminylthiosemicarbazone. Z. Anorg. Allg. Chem. 2004, 630, 1096–1109. [Google Scholar] [CrossRef]
  40. Orlewska, C.; Foks, H.; Sowinski, P.; Martynowski, D.; Olczak, A.; Glowka, M.L. Synthesis, structure and tuberculostatic activity of N’-(amino-pyridal-methylene)-hydrazinecarbodithioic acid methyl esters. Polish J. Chem. 2001, 75, 1237–1245. [Google Scholar]
  41. Ketcham, K.A.; Swearingen, J.K.; Castineiras, A.; Garcia, I.; Bermejo, E.; West, D.X. Iron(III), cobalt(II,III), copper(II) and zinc(II) complexes of 2-pyridineformamide 3-piperidylthiosemicarbazone. Polyhedron 2001, 20, 3265–3273. [Google Scholar] [CrossRef]
  42. Bermejo, E.; Castiñeiras, A.; Fostiak, L.M.; García, I.; Llamas-Saiz, A.L.; Swearingen, J.K.; West, D.X. Synthesis, Characterization and Molecular Structure of 2-Pyridyl-formamide N(4)-Dimethylthiosemicarbazone and Some Five-Coordinated Zinc(II) and Cadmium(II) Complexes. Z. Naturforsch. 2001, 56, 1297–1305. [Google Scholar] [CrossRef]
  43. Bermejo, E.; Castineiras, A.; Garcia-Santos, I.; Fostiak, L.M.; Swearingen, J.K.; West, D.X. Spectral and Structural Studies of Transition Metal Complexes of 2-Pyridineformamide N(4)-ethylthiosemicarbazone. Z. Anorg. Allg. Chem. 2005, 631, 728–738. [Google Scholar] [CrossRef]
  44. Castineiras, A.; Garcia-Santos, I.; Nogueiras, S.; Rodriguez-Gonzalez, I.; Rodriguez-Riobo, R. Supramolecular interactions in biologically relevant compounds. 2-Pyrazineformamide thiosemicarbazones and some products of their cyclization. J. Mol. Struct. 2014, 1074, 1–18. [Google Scholar] [CrossRef]
  45. Amim, R.S.; Firmino, G.S.S.; Rego, A.C.P.D.; Nery, A.L.; Da-Silva, S.A.G.; de Souza, M.V.N.; Pessoa, C.; Resende, J.A.L.C.; Lessa, J.D.F.-V.J.A. Cytotoxicity and Leishmanicidal Activity of Isoniazid-Derived Hydrazones and 2-Pyrazineformamide Thiosemicarbazones. J. Braz. Chem. Soc. 2016, 27, 769–777. [Google Scholar] [CrossRef]
  46. Castineiras, A.; Gacia, I.; Bermejo, E.; West, D.X. Structural and Spectral Studies of 2-Pyridineformamide Thiosemicarbazone and its Complexes Prepared with Zinc Halides. Z. Naturforsch. B Chem. Sci. 2000, 55, 511. [Google Scholar] [CrossRef]
  47. Francuski, B.M.; Novakovic, S.B.; Francuski, D.D.; Bogdanovic, G.A. Charge Density Analysis of 2-Pyridineformamide N(4)-Methylthiosemicarbazone (Z′ = 4): Role of an Enhanced N–H···S Thioureido Dimer. Cryst. Growth Des. 2017, 17, 2993–3004. [Google Scholar] [CrossRef]
  48. Favre-Nicolin, V.; Černý, R. FOX, ‘free Objects for Crystallography’: A Modular Approach to Ab Initio Structure Determination from Powder Diffraction. J. Appl. Cryst. 2002, 35, 734–743. [Google Scholar] [CrossRef] [Green Version]
  49. Toby, B.H.; Von Dreele, R.B. GSAS-II: The Genesis of a Modern Open-Source All Purpose Crystallography Software Package. J. Appl. Cryst. 2013, 46, 544–549. [Google Scholar] [CrossRef]
  50. Bruno, I.J.; Cole, J.C.; Kessler, M.; Luo, J.; Motherwell, W.D.S.; Purkis, L.H.; Smith, B.R.; Taylor, R.; Cooper, R.I.; Harris, S.E.; et al. Retrieval of Crystallographically-Derived Molecular Geometry Information. J. Chem. Inf. Comput. Sci. 2004, 44, 2133–2144. [Google Scholar] [CrossRef]
Materials 15 00349 g001 550
Figure 1. Intramolecular hydrogen bonds formed in the molecules of S,S-diesters of picolinoylcarbonohydrazodithioic acid and amino(pyridin-2-yl)methylenecarbonohydrazodithioic acid.
Figure 1. Intramolecular hydrogen bonds formed in the molecules of S,S-diesters of picolinoylcarbonohydrazodithioic acid and amino(pyridin-2-yl)methylenecarbonohydrazodithioic acid.
Materials 15 00349 g001
Materials 15 00349 g002 550
Figure 2. Structural formulas of the studied compounds.
Figure 2. Structural formulas of the studied compounds.
Materials 15 00349 g002
Materials 15 00349 sch001 550
Scheme 1. Synthesis of 2-[amino-(4-phenylthiopyridin-2-yl)methylene]-N-cyclohexylhydrazinecarbothioamide 1. (I) thiophenol (1.2 equiv.), dioxane, DBU, reflux, 1 h; (II) methanol, DBU, reflux, 4 h; then hydrazine hydrate, reflux, 15 min; (III) cyclohexyl isothiocyanate (1.1 equiv.), reflux, 5 min.
Scheme 1. Synthesis of 2-[amino-(4-phenylthiopyridin-2-yl)methylene]-N-cyclohexylhydrazinecarbothioamide 1. (I) thiophenol (1.2 equiv.), dioxane, DBU, reflux, 1 h; (II) methanol, DBU, reflux, 4 h; then hydrazine hydrate, reflux, 15 min; (III) cyclohexyl isothiocyanate (1.1 equiv.), reflux, 5 min.
Materials 15 00349 sch001
Materials 15 00349 g003a 550Materials 15 00349 g003b 550Materials 15 00349 g003c 550
Figure 3. The molecular structures of compounds 1–6, showing the atom-labeling schemes. Displacement ellipsoids are drawn at the 50% probability level, and H atoms are shown as small spheres of arbitrary radii. Drawing prepared with Mercury software.
Figure 3. The molecular structures of compounds 1–6, showing the atom-labeling schemes. Displacement ellipsoids are drawn at the 50% probability level, and H atoms are shown as small spheres of arbitrary radii. Drawing prepared with Mercury software.
Materials 15 00349 g003aMaterials 15 00349 g003bMaterials 15 00349 g003c
Materials 15 00349 g004 550
Figure 4. The intermolecular hydrogen bonds in compound 1.
Figure 4. The intermolecular hydrogen bonds in compound 1.
Materials 15 00349 g004
Materials 15 00349 g005 550
Figure 5. The intermolecular hydrogen bonds in compound 2.
Figure 5. The intermolecular hydrogen bonds in compound 2.
Materials 15 00349 g005
Materials 15 00349 g006 550
Figure 6. The intermolecular hydrogen bonds in compound 3.
Figure 6. The intermolecular hydrogen bonds in compound 3.
Materials 15 00349 g006
Materials 15 00349 g007 550
Figure 7. The intermolecular hydrogen bonds in compound 4.
Figure 7. The intermolecular hydrogen bonds in compound 4.
Materials 15 00349 g007
Materials 15 00349 g008 550
Figure 8. The crystal packing of compound 4.
Figure 8. The crystal packing of compound 4.
Materials 15 00349 g008
Materials 15 00349 g009 550
Figure 9. The intermolecular hydrogen bonds in compound 5.
Figure 9. The intermolecular hydrogen bonds in compound 5.
Materials 15 00349 g009
Materials 15 00349 g010 550
Figure 10. The intermolecular hydrogen bonds in compound 6.
Figure 10. The intermolecular hydrogen bonds in compound 6.
Materials 15 00349 g010
Materials 15 00349 g011 550
Figure 11. Overlay of molecules for all determined structures; 1—red and orange, 2—light green and green, 3—magenta, 4—light blue and blue, 5—grey and 6—purple.
Figure 11. Overlay of molecules for all determined structures; 1—red and orange, 2—light green and green, 3—magenta, 4—light blue and blue, 5—grey and 6—purple.
Materials 15 00349 g011
Materials 15 00349 g012 550
Figure 12. Overlay of molecules for analog structures from CSD (zwitterion form (YACFOY [39], WEXSUO [11], WEXSOI [11], SUZGUP [40], PASZEP [12], MEVNIK [41], BEWLOH [7], ADABIQ [42])—blue, neutral form (YAPJUV [43], XOTWUY01 [44], WOGGAC [44], WOGFUV [44], UYOSIM [45], MEMWUW [46], DIFROZ01 [47])—red).
Figure 12. Overlay of molecules for analog structures from CSD (zwitterion form (YACFOY [39], WEXSUO [11], WEXSOI [11], SUZGUP [40], PASZEP [12], MEVNIK [41], BEWLOH [7], ADABIQ [42])—blue, neutral form (YAPJUV [43], XOTWUY01 [44], WOGGAC [44], WOGFUV [44], UYOSIM [45], MEMWUW [46], DIFROZ01 [47])—red).
Materials 15 00349 g012
Materials 15 00349 g013 550
Figure 13. Overlay of molecules for analog structures from CSD (zwitterion form—blue, neutral form—red).
Figure 13. Overlay of molecules for analog structures from CSD (zwitterion form—blue, neutral form—red).
Materials 15 00349 g013
Materials 15 00349 g014 550
Figure 14. 15N CP MAS NMR spectra of compound 1 (a) and compound 4 (b), recorded at a spinning rate of 8 kHz and 1H Larmor frequency of 400.1 MHz at ambient temperature. Assignments are colored blue and indicate –N = NH- positions by green rectangles.
Figure 14. 15N CP MAS NMR spectra of compound 1 (a) and compound 4 (b), recorded at a spinning rate of 8 kHz and 1H Larmor frequency of 400.1 MHz at ambient temperature. Assignments are colored blue and indicate –N = NH- positions by green rectangles.
Materials 15 00349 g014
Materials 15 00349 g015 550
Figure 15. 15N CP NMR solution spectra in DMSO-d6 of compound 1 (a) and compound 4 (b), recorded at 298 K.
Figure 15. 15N CP NMR solution spectra in DMSO-d6 of compound 1 (a) and compound 4 (b), recorded at 298 K.
Materials 15 00349 g015
Table
Table 1. Tuberculostatic activity of 2-amidinothiosemicarbazone pyridine derivatives 1–6 [14].
Table 1. Tuberculostatic activity of 2-amidinothiosemicarbazone pyridine derivatives 1–6 [14].
CompoundMIC [µg/mL] *
H37RvSpec.210
12550
212.512.5
36.256.25
46.266.25
56.256.25
60.40.4
INH3.112.5
PZA25>400
* MIC-minimum inhibitory concentration, H37Rv-standard strain of M. tuberculosis, Spec. 210 strain of M. tuberculosis resistant to isoniazid, rifampicin, ethambutol and p-aminosalicylic acid.
Table
Table 2. Crystal data, data collection and refinement details.
Table 2. Crystal data, data collection and refinement details.
Compound123456
Chemical formulaC19H23N5S2C18H21N5S2C15H22N6OSC21H27N7OSC3H7NO·H2OC21H27N7SC15H22N6OS
Mr385.54371.52334.45516.67409.56334.45
Crystal system, space groupTriclinic, P-1Monoclinic, P21/nMonoclinic, P21/nTriclinic, P-1Triclinic, P-1Monoclinic, P21/c
Temperature (K)100100110120100100
a, b, c (Å)9.9607 (1), 11.4800 (2), 17.9470 (2)9.63579 (7), 11.57077 (6), 32.5353 (2)9.6759 (2), 15.3925 (4), 11.4019 (3)9.1277 (5), 9.2367 (5), 35.3349 (19)6.5854 (3), 7.8371 (3), 19.5806 (6)9.7946 (1), 12.8549 (2), 12.6723 (2)
α, β, γ (°)79.552 (1), 75.589 (1), 85.158 (1)90, 97.4227 (7), 9090, 97.977 (1), 9090.778 (2), 92.528 (2), 116.791 (3)95.660 (3), 95.484 (3), 98.811 (3)90, 91.200 (1), 90
V (Å3)1953.03 (5)3597.08 (4)1681.73 (7)2654.8 (3)987.46 (7)1595.20 (4)
Z (Z’)4 (2)8 (2)4 (1)4 (2)2 (1)4 (1)
μ (mm−1)2.572.771.831.421.641.93
Crystal size (mm)0.81 × 0.42 × 0.200.81 × 0.38 × 0.070.85 × 0.40 × 0.251.06 × 0.50 × 0.210.17 × 0.05 × 0.030.55 × 0.4 × 0.3
No. of measured, independent and observed [I > 2σ(I)] reflections39,531, 8158, 7475174,670, 7668, 718617,901, 3252, 321642,785, 10,708, 98839850, 3829, 324316,851, 3188, 3010
Rint0.0540.0820.0220.0370.0410.030
(sin θ/λ)max (Å−1)0.6370.6370.6180.6260.6350.637
R[F2 > 2σ(F2)], wR(F2), S0.040, 0.110, 1.100.055, 0.153, 1.190.031, 0.082, 1.050.065, 0.155, 1.250.040, 0.108, 1.060.030, 0.080, 1.05
No. of parameters494471266772274220
Δmax, Δmin (e Å−3)0.39, −0.360.77, −0.460.21, −0.320.54, −0.330.41, −0.330.24, −0.25
Table
Table 3. Hydrogen-bond geometry (Å, °) for compound 1.
Table 3. Hydrogen-bond geometry (Å, °) for compound 1.
D—H···AD—HH···AD···AD—H···A
N4A—H4AA···S1B i0.85 (3)2.47 (3)3.2938 (16)166 (3)
N4A—H4AB···N42A0.86 (3)2.30 (3)2.676 (2)107 (2)
N4A—H4AB···N42B0.86 (3)2.53 (3)3.256 (2)142 (3)
N2A—H2A···S1B i0.85 (2)2.65 (2)3.4953 (14)176.1 (18)
N2B—H2B···S1A ii0.84 (2)2.67 (2)3.4657 (14)158.1 (18)
N4B—H4BA···N42B0.83 (2)2.26 (2)2.648 (2)108.8 (19)
N4B—H4BB···S1A ii0.86 (2)2.43 (2)3.2872 (16)173 (2)
Symmetry codes: (i) x, y + 1, z; (ii) x, y − 1, z.
Table
Table 4. Hydrogen-bond geometry (Å, °) for compound 2.
Table 4. Hydrogen-bond geometry (Å, °) for compound 2.
D—H···AD—HH···AD···AD—H···A
N4B—H4BA···N2B0.84 (5)2.30 (5)2.702 (3)109 (4)
N4B—H4BB···S1 B i0.90 (5)2.53 (5)3.423 (3)171 (4)
N4A—H4AA···N2A0.85 (4)2.31 (4)2.691 (4)108 (4)
N3A—H3A···S1A0.84 (5)2.35 (4)2.827 (2)116 (3)
N3A—H3A···N42A0.84 (5)2.27 (4)2.636 (3)106 (3)
N3B—H3B···S1B0.90 (5)2.30 (4)2.839 (2)119 (3)
N3B—H3B···N42B0.90 (5)2.32 (4)2.660 (3)103 (3)
N4A—H4AB···S1A ii0.87 (4)2.49 (4)3.348 (3)170 (4)
Symmetry codes: (i) −x + 3/2, y + 1/2, −z + 1/2; (ii) −x + 1/2, y − 1/2, −z + 1/2.
Table
Table 5. Hydrogen-bond geometry (Å, °) for compound 3.
Table 5. Hydrogen-bond geometry (Å, °) for compound 3.
D—H···AD—HH···AD···AD—H···A
N4—HbB···S1 i0.911 (18)2.350 (18)3.2508 (11)169.7 (15)
N4—H4A···N20.876 (17)2.213 (17)2.6222 (15)108.2 (12)
Symmetry codes: (i) x + 1/2, −y + 1/2, z − 1/2.
Table
Table 6. Hydrogen-bond geometry (Å, °) for 4.
Table 6. Hydrogen-bond geometry (Å, °) for 4.
D—H···AD—HH···AD···AD—H···A
N4B—H4BA···O0D0.882.203.003 (4)152
O1A—H1A···O54X0.82 (5)2.04 (6)2.85 (2)170 (5)
O1A—H1A···O54A0.82 (5)2.02 (6)2.84 (3)178 (7)
O1B—H1B···S1B i0.92 (5)2.34 (5)3.247 (3)171 (4)
N4B—H4BB···O1B0.881.992.827 (4)159
O1A—H2A···S1A ii0.94 (6)2.30 (6)3.232 (4)172 (4)
O1B—H2B···O54Q iii0.84 (6)2.21 (6)3.025 (19)164 (5)
O1B—H2B···O54B iii0.84 (6)1.90 (6)2.70 (3)161 (5)
N4A—H4AA···O1M0.882.172.969 (4)150
N4A—H4AB···O1A iv0.881.992.831 (4)160
Symmetry codes: (i) x + 1, y + 1, z; (ii) −x, −y + 1, −z + 1; (iii) −x + 3, −y + 2, −z; (iv) −x, −y + 2, −z + 1.
Table
Table 7. Hydrogen-bond geometry (Å, °) for compound 5.
Table 7. Hydrogen-bond geometry (Å, °) for compound 5.
D—H···AD—HH···AD···AD—H···A
N3—H3···S10.94 (3)2.29 (2)2.8351 (14)116.5 (18)
N4—H4B···S1 i0.87 (3)2.70 (3)3.4792 (16)150 (2)
Symmetry code: (i) x + 1, y, z.
Table
Table 8. Hydrogen-bond geometry (Å, °) for compound 6.
Table 8. Hydrogen-bond geometry (Å, °) for compound 6.
D—H···AD—HH···AD···AD—H···A
N3—H3···S10.871 (18)2.395 (17)2.8596 (10)113.8 (13)
N3—H3···N420.871 (18)2.237 (17)2.6219 (14)106.6 (13)
N4—H4A···N20.828 (19)2.332 (18)2.6827 (15)106.1 (14)
N4—H4A···S1 i0.828 (19)2.679 (19)3.3937 (11)145.5 (16)
N4—H4B···O14 ii0.855 (19)2.176 (18)2.9458 (14)149.8 (17)
Symmetry codes: (i) x, −y + 1/2, z − 1/2; (ii) −x, y + 1/2, −z + 1/2.
Table
Table 9. 15N CP MAS NMR experimental and GIPAW calculated chemical shifts (δ) [in ppm] (recalculated from GIPAW nuclear shieldings from equation δ = 230 − σGIPAW) for compound 1 with the hydrogen attached to the nitrogen in position N2 or N3 in the crystal structure.
Table 9. 15N CP MAS NMR experimental and GIPAW calculated chemical shifts (δ) [in ppm] (recalculated from GIPAW nuclear shieldings from equation δ = 230 − σGIPAW) for compound 1 with the hydrogen attached to the nitrogen in position N2 or N3 in the crystal structure.
Structure
(Position)		δExpδGIPAW
(Hydrogen on N2)		δGIPAW
(Hydrogen on N3)
N2A166.3173.1256.6
N2B166.3177.9247.4
N3A231.8230.4176.1
N3B231.8230.2165.1
N4A80.078.886.8
N4B80.079.695.0
N5A138.2136.2114.3
N5B138.2135.1119.0
N42A292.9288.2276.2
N42B292.9282.7286.0
Table
Table 10. 15N CP MAS NMR experimental and GIPAW calculated chemical shifts (δ) [in ppm] (recalculated from GIPAW nuclear shieldings from equation δ = 230 − σGIPAW) for compound 4 with the hydrogen attached to the nitrogen in position N2 or N3 in the crystal structure.
Table 10. 15N CP MAS NMR experimental and GIPAW calculated chemical shifts (δ) [in ppm] (recalculated from GIPAW nuclear shieldings from equation δ = 230 − σGIPAW) for compound 4 with the hydrogen attached to the nitrogen in position N2 or N3 in the crystal structure.
Structure
(Position)		δExpδGIPAW
(Hydrogen on N2)		δGIPAW
(Hydrogen on N3)
N2A235.3174.4237.9
N2B239.33171.9237.6
N3A184.3248.2183.6
N3B184.3246.0181.5
N4A88.678.392.2
N4B91.381.594.1
N11A107.7111.8115.0
N11B107.7115.0110.8
N14A74.973.478.9
N14B76.576.376.0
N42A269.4282.9262.2
N42B270.8281.6263.6
N51A84.576.682.4
N51B84.577.982.0
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Gobis, K.; Szczesio, M.; Olczak, A.; Pawlak, T.; Augustynowicz-Kopeć, E.; Krause, M.; Główka, M.L. Relationship between the Crystal Structure and Tuberculostatic Activity of Some 2-Amidinothiosemicarbazone Derivatives of Pyridine. Materials 2022, 15, 349. https://doi.org/10.3390/ma15010349

AMA Style

Gobis K, Szczesio M, Olczak A, Pawlak T, Augustynowicz-Kopeć E, Krause M, Główka ML. Relationship between the Crystal Structure and Tuberculostatic Activity of Some 2-Amidinothiosemicarbazone Derivatives of Pyridine. Materials. 2022; 15(1):349. https://doi.org/10.3390/ma15010349

Chicago/Turabian Style

Gobis, Katarzyna, Małgorzata Szczesio, Andrzej Olczak, Tomasz Pawlak, Ewa Augustynowicz-Kopeć, Malwina Krause, and Marek L. Główka. 2022. "Relationship between the Crystal Structure and Tuberculostatic Activity of Some 2-Amidinothiosemicarbazone Derivatives of Pyridine" Materials 15, no. 1: 349. https://doi.org/10.3390/ma15010349

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop