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Article

Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves

1
Department of Mechanical Engineering and Agrophysics, Faculty of Production Engineering and Energetics, University of Agriculture in Krakow, Balicka 120, 30-149 Krakow, Poland
2
Institute of Health, State Higher Vocational School in Nowy Sacz, Staszica 1, 33-300 Nowy Sacz, Poland
3
Department of Bioorganic Chemistry, Chair of Organic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland
4
Department of Machinery Exploitation, Ergonomics and Production Processes, Faculty of Production Engineering and Energetics, University of Agriculture in Krakow, Balicka 116 B, 30-149 Krakow, Poland
5
Department of Toxicology, Collegium Medicum, Jagiellonian University, Medyczna 9, 30-688 Kraków, Poland
6
Department of Biotechnology and General Food Technology, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland
7
Department of Biochemistry, Faculty of Science, Ege University, Izmir 35040, Turkey
*
Author to whom correspondence should be addressed.
Materials 2020, 13(24), 5811; https://doi.org/10.3390/ma13245811
Submission received: 17 November 2020 / Revised: 3 December 2020 / Accepted: 16 December 2020 / Published: 19 December 2020
(This article belongs to the Special Issue Complex Biomaterials Systems and Their Applications)

Abstract

The influence of harvest period and drying method of Salvia officinalis L. leaves on the content of essential oils, polyphenols and antioxidant properties was investigated. Sage leaves were collected twice during plant blossoming (in June and July) and dried traditionally in natural conditions and at 35 °C. Antioxidant activity was assayed in methanol-acetone extracts and infusions of dried leaves with the use of free radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP) technique. Total phenolic content in extracts as well as in infusions was determined by the means of Folin–Ciocalteu method. Based on the LC/MS analysis, the polyphenol compounds present in both extracts and in infusions were identified. The extracts contained more polyphenols and were characterized by higher antioxidant activity than infusions. In the extracts significant amount of ferulic acid was found, which was influenced both by the harvest period and drying method. The amount of ferulic acid found in extracts obtained from the June leaves dried traditionally was three times lower (6.185 μg/g DW) than in extracts from July leaves dried in the same conditions. Harvest period had a significant effect on the essential oils’ content, leaves collected in July contained 15% more oils than those collected in June.
Keywords: antioxidants; chromatographic analysis; DPPH; FRAP; phenols; Salvia officinalis antioxidants; chromatographic analysis; DPPH; FRAP; phenols; Salvia officinalis

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MDPI and ACS Style

Francik, S.; Francik, R.; Sadowska, U.; Bystrowska, B.; Zawiślak, A.; Knapczyk, A.; Nzeyimana, A. Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials 2020, 13, 5811. https://doi.org/10.3390/ma13245811

AMA Style

Francik S, Francik R, Sadowska U, Bystrowska B, Zawiślak A, Knapczyk A, Nzeyimana A. Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials. 2020; 13(24):5811. https://doi.org/10.3390/ma13245811

Chicago/Turabian Style

Francik, Sławomir, Renata Francik, Urszula Sadowska, Beata Bystrowska, Agnieszka Zawiślak, Adrian Knapczyk, and Abdul Nzeyimana. 2020. "Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves" Materials 13, no. 24: 5811. https://doi.org/10.3390/ma13245811

APA Style

Francik, S., Francik, R., Sadowska, U., Bystrowska, B., Zawiślak, A., Knapczyk, A., & Nzeyimana, A. (2020). Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves. Materials, 13(24), 5811. https://doi.org/10.3390/ma13245811

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