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Open AccessArticle

Enzymatically Functionalized Composite Materials Based on Nanocellulose and Poly(Vinyl Alcohol) Cryogel and Possessing Antimicrobial Activity

1
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
2
N.M.Emanuel Institute of Biochemical Physics RAS, Moscow 119334, Russia
3
A.N.Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119334, Russia
*
Author to whom correspondence should be addressed.
Materials 2019, 12(21), 3619; https://doi.org/10.3390/ma12213619
Received: 27 September 2019 / Revised: 28 October 2019 / Accepted: 31 October 2019 / Published: 4 November 2019
In the present work, innovative composite biomaterials possessing bactericidal properties and based on the hexahistidine-tagged organophosphorus hydrolase (His6-OPH) entrapped in the poly(vinyl alcohol) cryogel (PVA-CG)/bacterial cellulose (BC) were developed. His6-OPH possesses lactonase activity, with a number of N-acyl homoserine lactones being the inducers of Gram-negative bacterial resistance. The enzyme can also be combined with various antimicrobial agents (antibiotics and antimicrobial peptides) to improve the efficiency of their action. In this study, such an effect was shown for composite biomaterials when His6-OPH was entrapped in PVA-CG/BC together with β-lactam antibiotic meropenem or antimicrobial peptides temporin A and indolicidin. The residual catalytic activity of immobilized His6-OPH was 60% or more in all the composite samples. In addition, the presence of BC filler in the PVA-CG composite resulted in a considerable increase in the mechanical strength and heat endurance of the polymeric carrier compared to the BC-free cryogel matrix. Such enzyme-containing composites could be interesting in the biomedical field to help overcome the problem of antibiotic resistance of pathogenic microorganisms. View Full-Text
Keywords: immobilized hexahistidine-tagged organophosphorus hydrolase; poly(vinyl alcohol) cryogel; bacterial cellulose; β-lactam antibiotic; antimicrobial peptides; bactericidal activity immobilized hexahistidine-tagged organophosphorus hydrolase; poly(vinyl alcohol) cryogel; bacterial cellulose; β-lactam antibiotic; antimicrobial peptides; bactericidal activity
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Aslanli, A.; Stepanov, N.; Razheva, T.; Podorozhko, E.A.; Lyagin, I.; Lozinsky, V.I.; Efremenko, E. Enzymatically Functionalized Composite Materials Based on Nanocellulose and Poly(Vinyl Alcohol) Cryogel and Possessing Antimicrobial Activity. Materials 2019, 12, 3619.

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