Next Article in Journal
Water Uptake and Transport Properties of La1−xCaxScO3−α Proton-Conducting Oxides
Previous Article in Journal
Chain Extension and Synergistic Flame-Retardant Effect of Aromatic Schiff Base Diepoxide on Polyamide 6/Aluminum Diethylphosphinate Composites
Article Menu

Export Article

Open AccessArticle

Understanding the Influence of a Bifunctional Polyethylene Glycol Derivative in Protein Corona Formation around Iron Oxide Nanoparticles

1
School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
2
Centro Nacional de Biotecnología (CNB)/CSIC, Darwin, 3, 28049 Madrid, Spain
3
Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC/Universidad de Zaragoza, C/Pedro Cerbuna 12, 50009 Zaragoza, Spain
4
Centro de Biología Molecular “Severo Ochoa” (CBMSO)/UAM-CSIC, Nicolás Cabrera, 1, 28049 Madrid, Spain
5
Instituto de Ciencia de Materiales de Madrid (ICMM)/CSIC, Sor Juana Inés de la Cruz 3, Cantoblanco, 28049 Madrid, Spain
6
Faculty of Pharmacy, Complutense University of Madrid (UCM), Plaza Ramón y Cajal s/n, 28040 Madrid, Spain
7
School of Medicine, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
*
Authors to whom correspondence should be addressed.
Present address: School of Pharmacy, Queen’s University Belfast, 97 Lisburn Rd, Belfast BT9 7BL, UK.
Materials 2019, 12(14), 2218; https://doi.org/10.3390/ma12142218
Received: 21 June 2019 / Revised: 6 July 2019 / Accepted: 8 July 2019 / Published: 10 July 2019
(This article belongs to the Special Issue Magnetic Nanoparticles for Biomedical Applications)
  |  
PDF [2485 KB, uploaded 10 July 2019]
  |     |  

Abstract

Superparamagnetic iron oxide nanoparticles are one of the most prominent agents used in theranostic applications, with MRI imaging the main application assessed. The biomolecular interface formed on the surface of a nanoparticle in a biological medium determines its behaviour in vitro and in vivo. In this study, we have compared the formation of the protein corona on highly monodisperse iron oxide nanoparticles with two different coatings, dimercaptosuccinic acid (DMSA), and after conjugation, with a bifunctional polyethylene glycol (PEG)-derived molecule (2000 Da) in the presence of Wistar rat plasma. The protein fingerprints around the nanoparticles were analysed in an extensive proteomic study. The results presented in this work indicate that the composition of the protein corona is very difficult to predict. Proteins from different functional categories—cell components, lipoproteins, complement, coagulation, immunoglobulins, enzymes and transport proteins—were identified in all samples with very small variability. Although both types of nanoparticles have similar amounts of bonded proteins, very slight differences in the composition of the corona might explain the variation observed in the uptake and biotransformation of these nanoparticles in Caco-2 and RAW 264.7 cells. Cytotoxicity was also studied using a standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Controlling nanoparticles’ reactivity to the biological environment by deciding on its surface functionalization may suggest new routes in the control of the biodistribution, biodegradation and clearance of multifunctional nanomedicines. View Full-Text
Keywords: iron oxide nanoparticles; protein corona; PEG-coated nanoparticles iron oxide nanoparticles; protein corona; PEG-coated nanoparticles
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Ruiz, A.; Alpízar, A.; Beola, L.; Rubio, C.; Gavilán, H.; Marciello, M.; Rodríguez-Ramiro, I.; Ciordia, S.; Morris, C.J.; Morales, M.P. Understanding the Influence of a Bifunctional Polyethylene Glycol Derivative in Protein Corona Formation around Iron Oxide Nanoparticles. Materials 2019, 12, 2218.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top