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Open AccessArticle

In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material

1
Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
2
Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
3
Department of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
4
Department of Orthopaedic Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
*
Author to whom correspondence should be addressed.
Materials 2019, 12(11), 1881; https://doi.org/10.3390/ma12111881
Received: 18 April 2019 / Revised: 6 June 2019 / Accepted: 10 June 2019 / Published: 11 June 2019
(This article belongs to the Special Issue Design of Materials for Bone Tissue Scaffolds)
We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)—a commercially available β-TCP bone filler—the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone. View Full-Text
Keywords: starfish; calcium carbonate; porous calcium phosphate; β-tricalcium phosphate; bone substitute; angiogenesis starfish; calcium carbonate; porous calcium phosphate; β-tricalcium phosphate; bone substitute; angiogenesis
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Ishida, H.; Haniu, H.; Takeuchi, A.; Ueda, K.; Sano, M.; Tanaka, M.; Takizawa, T.; Sobajima, A.; Kamanaka, T.; Saito, N. In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material. Materials 2019, 12, 1881.

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