Next Article in Journal
Three-Dimensional Characterization of Hardened Paste of Hydrated Tricalcium Silicate by Serial Block-Face Scanning Electron Microscopy
Previous Article in Journal
α-Fe2O3 Nanoparticles/Vermiculite Clay Material: Structural, Optical and Photocatalytic Properties
Article Menu
Issue 11 (June-1) cover image

Export Article

Open AccessArticle

In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material

1
Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
2
Department of Biomedical Engineering, Graduate School of Medicine, Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
3
Department of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
4
Department of Orthopaedic Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
*
Author to whom correspondence should be addressed.
Materials 2019, 12(11), 1881; https://doi.org/10.3390/ma12111881
Received: 18 April 2019 / Revised: 6 June 2019 / Accepted: 10 June 2019 / Published: 11 June 2019
(This article belongs to the Special Issue Design of Materials for Bone Tissue Scaffolds)
  |  
PDF [10576 KB, uploaded 11 June 2019]
  |  

Abstract

We evaluated starfish-derived β-tricalcium phosphate (Sf-TCP) obtained by phosphatization of starfish-bone-derived porous calcium carbonate as a potential bone substitute material. The Sf-TCP had a communicating pore structure with a pore size of approximately 10 μm. Although the porosity of Sf-TCP was similar to that of Cerasorb M (CM)—a commercially available β-TCP bone filler—the specific surface area was roughly three times larger than that of CM. Observation by scanning electron microscopy showed that pores communicated to the inside of the Sf-TCP. Cell growth tests showed that Sf-TCP improved cell proliferation compared with CM. Cells grown on Sf-TCP showed stretched filopodia and adhered; cells migrated both to the surface and into pores. In vivo, vigorous tissue invasion into pores was observed in Sf-TCP, and more fibrous tissue was observed for Sf-TCP than CM. Moreover, capillary formation into pores was observed for Sf-TCP. Thus, Sf-TCP showed excellent biocompatibility in vitro and more vigorous bone formation in vivo, indicating the possible applications of this material as a bone substitute. In addition, our findings suggested that mimicking the microstructure derived from whole organisms may facilitate the development of superior artificial bone. View Full-Text
Keywords: starfish; calcium carbonate; porous calcium phosphate; β-tricalcium phosphate; bone substitute; angiogenesis starfish; calcium carbonate; porous calcium phosphate; β-tricalcium phosphate; bone substitute; angiogenesis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Ishida, H.; Haniu, H.; Takeuchi, A.; Ueda, K.; Sano, M.; Tanaka, M.; Takizawa, T.; Sobajima, A.; Kamanaka, T.; Saito, N. In Vitro and In Vivo Evaluation of Starfish Bone-Derived β-Tricalcium Phosphate as a Bone Substitute Material. Materials 2019, 12, 1881.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top