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Article

Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution

1
Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394, Japan
2
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Magdalenka, Poland
3
Department of Functional Morphology, Division of Cell Regeneration and Transplantation, Nihon University School of Medicine, Tokyo 173-8610, Japan
*
Author to whom correspondence should be addressed.
Materials 2019, 12(11), 1759; https://doi.org/10.3390/ma12111759
Received: 3 April 2019 / Revised: 17 May 2019 / Accepted: 22 May 2019 / Published: 30 May 2019
(This article belongs to the Special Issue New Materials and Technologies for Guided Tissue Regeneration)
To better understand the regulation and function of cellular interactions, three-dimensional (3D) assemblies of single cells and subsequent functional analysis are gaining popularity in many research fields. While we have developed strategies to build stable cellular structures using optical tweezers in a minimally invasive state, methods for manipulating a wide range of cell types have yet to be established. To mimic organ-like structures, the construction of 3D cellular assemblies with variety of cell types is essential. Our recent studies have shown that the presence of nonspecific soluble polymers in aqueous solution is the key to creating stable 3D cellular assemblies efficiently. The present study further expands on the construction of 3D single cell assemblies using two different cell types. We have successfully generated 3D cellular assemblies, using GFP-labeled adipose tissue-derived stem cells and endothelial cells by using optical tweezers. Our findings will support the development of future applications to further characterize cellular interactions in tissue regeneration. View Full-Text
Keywords: mesenchymal stem cells; endothelial cells; three-dimensional assemblies; composites with different cell types; depletion effect of polymer mesenchymal stem cells; endothelial cells; three-dimensional assemblies; composites with different cell types; depletion effect of polymer
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MDPI and ACS Style

Yamazaki, T.; Kishimoto, T.; Leszczyński, P.; Sadakane, K.; Kenmotsu, T.; Watanabe, H.; Kazama, T.; Matsumoto, T.; Yoshikawa, K.; Taniguchi, H. Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution. Materials 2019, 12, 1759. https://doi.org/10.3390/ma12111759

AMA Style

Yamazaki T, Kishimoto T, Leszczyński P, Sadakane K, Kenmotsu T, Watanabe H, Kazama T, Matsumoto T, Yoshikawa K, Taniguchi H. Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution. Materials. 2019; 12(11):1759. https://doi.org/10.3390/ma12111759

Chicago/Turabian Style

Yamazaki, Takehiro, Toshifumi Kishimoto, Paweł Leszczyński, Koichiro Sadakane, Takahiro Kenmotsu, Hirofumi Watanabe, Tomohiko Kazama, Taro Matsumoto, Kenichi Yoshikawa, and Hiroaki Taniguchi. 2019. "Construction of 3D Cellular Composites with Stem Cells Derived from Adipose Tissue and Endothelial Cells by Use of Optical Tweezers in a Natural Polymer Solution" Materials 12, no. 11: 1759. https://doi.org/10.3390/ma12111759

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