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TopUp SERS Substrates with Integrated Internal Standard
Open AccessFeature PaperArticle

Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection

1
CNR-IPCF, Istituto per i Processi Chimico-Fisici, Viale F. Stagno D’Alcontres 37, I-98168 Messina, Italy
2
Dottorato di Ricerca in Fisica, Università di Messina, Viale F. Stagno D’Alcontres 31, I-98166 Messina, Italy
3
Laboratoire CSPBAT, Université de Paris 13, Sorbonne Paris Cité, CNRS, 74 Rue Marcel-Cachin, F-93017 Bobigny, France
4
Institut des Molécules et Matériaux du Mans (IMMM-UMR CNRS 6283), Université du Mans, Avenue Olivier Messiaen, 72085 Le Mans, France
*
Author to whom correspondence should be addressed.
Now at LPICM, Ecole Polytechnique, CNRS, 91128 Palaiseau, France.
Now at CNR—Istituto di Fisica Applicata “Nello Carrara” (IFAC), I-50019 Sesto Fiorentino (FI), Italy.
Materials 2018, 11(3), 440; https://doi.org/10.3390/ma11030440
Received: 19 January 2018 / Revised: 12 March 2018 / Accepted: 15 March 2018 / Published: 17 March 2018
(This article belongs to the Special Issue SERS-Active Substrates)
Optical forces are used to aggregate plasmonic nanoparticles and create SERS–active hot spots in liquid. When biomolecules are added to the nanoparticles, high sensitivity SERS detection can be accomplished. Here, we pursue studies on Bovine Serum Albumin (BSA) detection, investigating the BSA–nanorod aggregations in a range from 100 µM to 50 nM by combining light scattering, plasmon resonance and SERS, and correlating the SERS signal with the concentration. Experimental data are fitted with a simple model describing the optical aggregation process. We show that BSA–nanorod complexes can be optically printed on non-functionalized glass surfaces, designing custom patterns stable with time. Furthermore, we demonstrate that this methodology can be used to detect catalase and hemoglobin, two Raman resonant biomolecules, at concentrations of 10 nM and 1 pM, respectively, i.e., well beyond the limit of detection of BSA. Finally, we show that nanorods functionalized with specific aptamers can be used to capture and detect Ochratoxin A, a fungal toxin found in food commodities and wine. This experiment represents the first step towards the addition of molecular specificity to this novel biosensor strategy. View Full-Text
Keywords: SERS; biosensor; gold nanoparticles; aptamers; toxins; hemeprotein; optical forces; optical tweezers; optical patterning; colloids SERS; biosensor; gold nanoparticles; aptamers; toxins; hemeprotein; optical forces; optical tweezers; optical patterning; colloids
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MDPI and ACS Style

Foti, A.; D’Andrea, C.; Villari, V.; Micali, N.; Donato, M.G.; Fazio, B.; Maragò, O.M.; Gillibert, R.; Lamy de la Chapelle, M.; Gucciardi, P.G. Optical Aggregation of Gold Nanoparticles for SERS Detection of Proteins and Toxins in Liquid Environment: Towards Ultrasensitive and Selective Detection. Materials 2018, 11, 440.

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