Evaluating the Performance of Biogas Plants Operating on Organic Waste: A Practical Approach
Abstract
1. Introduction
2. Types and Characteristics of Feedstock Used in Biogas Plants
2.1. High-Energy Feedstocks: Characteristics and Role in Biogas Yield
2.2. Medium and Low-Energy Feedstocks: Slurry and Liquid Substrates
2.3. Co-Digestion Strategies and Optimization of Feedstock Mixtures
- Achieving the appropriate C:N ratio in the substrate mix (ideally in the range of 20–30:1) [96].
- Ammonium nitrogen concentration should remain within an optimal range. According to Procházka et al. [97] optimal concentrations for methanogens were 2.1, 2.6 and 3.1 g/L of ammonia nitrogen in dependence on inoculum origin. Higher ammonium nitrogen content may increase the risk of ammonium inhibition, while too low NH4+-N concentrations can cause nitrogen starvation [98]. However, variations in operating temperature, reactor design, and the level of ammonia nitrogen inhibition tolerated by microbial communities across different systems preclude the definition of universal threshold values [99].
- Certain substrates, such as various types of straw or beet pulp, are significantly deficient in essential micronutrients and trace elements essential for the metabolism of microorganisms involved in the anaerobic digestion process. Therefore, substrates should be supplemented with those rich in these elements, such as animal manure [100].
- The average dry matter content of the substrate mix fed to fermenters in wet fermentation should be approximately 12%. A lower value will result in a low organic loading rate (OLR), and as a result, the anaerobic digestion potential of the fermenters will be poorly utilized [101].
- Depending on the substrate mixing technology used and its pumpability, a specific dry matter content (TS) level of the substrate mixture should not be exceeded. Some high-solids technologies allow AD at dry matter contents often exceeding 15% [102].
2.4. Influence of Feedstock Properties on Biogas Plant Performance
- Proper organic loading rate which is the amount of organic matter added to the fermenter daily. It is a key parameter controlling the efficiency and stability of a biogas plant. An OLR that is too high can lead to the accumulation of organic acids and acidification (and consequently to process failure), while an OLR that is too low can result in underutilization of reactor capacity. An appropriate OLR in the digester may vary depending on the substrate or substrate mixture used, temperature of the process and technology used [107,108]. For agricultural biogas plants, it typically should not exceed approximately 4–5 kg of VS/m3/d. Seick et al. [109] in laboratory conditions reached 5.8 g VS/L/day using maize silage and changing the regime from mesophilic to thermophilic conditions; Lindorfer et al. [110] in real-scale biogas plant conditions increased OLR from 2.11 to 4.25 kg of VS/m3/d in an agricultural biogas plant using pig manure, solid energy crops such as silages from maize and rye, ground grains from maize and wheat, and residues from vegetable processing as feedstock. Tamborrino et al. [111] described an installation in Italy fermenting olive waste (pulp and pitted olive pomace) and pulp pitted olive pomace and wheat bran shorts in the amount of 10% of the total powered biomass operated at an OLR of approximately 5.3–5.5 kg VS/m3/d. In turn, in the study by Jiang et al. [112] digested organic fraction of municipal solid waste (OFMSW) in a semi-continuous mode showed that the maximum achievable OLR was 9.00 g VS/L/d but the recommended operating conditions for the anaerobic digestion of OFMSW is an OLR of 7.50 g VS/L/d and thermophilic condition.
- Efficient mixing and substrate homogenization are essential for stable anaerobic digestion. Mechanical pretreatment such as grinding or chopping of biomass improves substrate accessibility for microorganisms and enhances the hydrolysis stage of the digestion process [113]. Particle size reduction increases the specific surface area of the substrate, facilitating microbial attachment and enzymatic degradation of organic matter. As reported in experimental studies, smaller particle sizes significantly improve methane production due to enhanced mass transfer and substrate availability for anaerobic microorganisms [114,115]. Mechanical pretreatment also improves reactor hydrodynamics and mixing efficiency. In industrial biogas plants, substrates are often milled or shredded before feeding to produce a pumpable slurry that can be effectively mixed in continuously stirred tank reactors. Adequate mixing ensures uniform distribution of microorganisms, nutrients and heat, which is essential for maintaining stable digestion performance [33,116].
- The absence of inhibitory compounds (e.g., antibiotics, toxic chemicals, excessive ammonium nitrogen). Antibiotics are among the most frequently reported inhibitors of anaerobic digestion because they directly affect microbial metabolism. These substances can disturb the activity of microorganisms responsible for the degradation of organic matter and the final stage of methane formation. According to Koniuszewska et al. [117] antibiotics in wastewater and cattle slurry used as substrates in the AD process can pose serious problems for anaerobic microorganisms, because they may reduce the activity of methanogenic microorganisms or alter the microbial community structure responsible for methane production [24]. Experimental studies confirm that many antibiotics can significantly reduce biogas production. For example, the addition of antimicrobial compounds to cattle manure during mesophilic anaerobic digestion caused substantial decreases in methane generation; in some cases, amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively [117]. Therefore, maintaining substrates free from inhibitory compounds is essential for stable biogas plant operation. In practice, this requires careful control of feedstock sources, avoiding wastes contaminated with pharmaceuticals, disinfectants, or toxic industrial chemicals. In cases where inhibitors are present, strategies such as dilution, co-digestion with clean substrates, or adsorption using materials like biochar may help mitigate their negative effects on the anaerobic digestion process [73,118]. Another inhibitor may be ammonium nitrogen, which is found in large quantities in poultry manure, which can affect the operation of biogas plant [119]. In this context, cooperation between the farmer supplying animal manure to the biogas plant and the biogas plant operator is crucial [120]. If a herd becomes ill and a veterinarian administers a strong dose of antibiotics to the animals, a significant portion of the antibiotic will be excreted by the animals and found in the slurry or manure. There are numerous cases where digested pulp containing antimicrobials and antibiotics reduces or even stops the fermentation process [121,122,123].
- Removal of mineral and other non-biodegradable contaminants from the feedstock is a critical step in the operation of biogas plants. Substrates used for anaerobic digestion, such as manure, agricultural residues, food waste or municipal organic waste, often contain inert materials including sand, stones, soil particles, glass, metals or plastics [124,125]. These materials are not degradable in the anaerobic digestion process and may lead to both operational and mechanical problems and can cause damage to mixers, pumps, or valves. In turn, Hoffmann et al. [126] observed a positive effect of sand on methane yield—sewage sludge degradation to CH4 was significantly enhanced in the presence of sand.
- The pH of the input substrate is generally of secondary importance, as a well-managed biogas plant maintains a stable pH in the individual fermentation reactors (maintained spontaneously by cooperating groups of bacteria). Unless substrate is fed too intensively, which would overload the fermenter and cause acidification. The appropriate pH level, is difficult to determine, it depends on the technology used and on whether the digestion process phases are separated. Ward et al. [127] assumed that the pH level suitable for methanogenesis ranges from 6.8 to 7.2; on the other hand Khalid et al. [128] assumed that the pH levels suitable for hydrolysis and acidogenesis are 5.5 and 6.5, respectively. Some experts (biogas plant operators) even recommend values for methanogenesis at pH level of 7.4–7.7.
3. Internal Consumption of Electricity and Heat in the Biogas Plant
- Building local heating pipelines and heating supply to nearby buildings [150];
- Building drying facilities for wood, agricultural products or digestate drying [151];
- Heating greenhouses [152];
- Combining biogas production with ethyl alcohol production [153];
- Producing cold from heat using trigeneration technology and utilizing the cold in industrial installations (dairies, cold stores, etc.) [154].
4. Energy Efficiency in Biogas Plant Operation
5. Discussion and Future Outlook
6. Conclusions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
| AD | Anaerobic Digestion |
| BMP | Biochemical Methane Potential |
| CHP | Combined Heat And Power |
| C:N | Carbon to Nitrogen Ratio |
| CNG | Compressed Natural Gas |
| COD | Chemical Oxygen Demand |
| DM | Dry Matter |
| EU | European Union |
| GHG | Greenhouse Gasses |
| HRT | Hydraulic Retention Time |
| IRENA | International Renewable Energy Agency |
| LCFAs | Long-Chain Fatty Acids |
| LNG | Liquefied Natural Gas |
| OFMSW | Organic Fraction of Municipal Solid |
| OLR | Organic Loading Rate |
| ORC | Organic Rankine Cycle |
| POME | Palm Oil Mill Effluent |
| RED III | Renewable Energy Directive III |
| VS | Volatile Solids |
| TS | Total Solids |
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| Region | 2015 | 2016 | 2017 | 2018 | 2019 | 2020 | 2021 | 2022 | 2023 | 2024 |
|---|---|---|---|---|---|---|---|---|---|---|
| Europe | 11,314 | 11,795 | 12,222 | 12,946 | 13,348 | 13,860 | 13,465 | 13,577 | 13,674 | 13,845 |
| South America | 301 | 367 | 397 | 421 | 512 | 590 | 633 | 672 | 700 | 712 |
| North America | 2622 | 2626 | 2730 | 2686 | 2649 | 2549 | 2521 | 2463 | 2340 | 2319 |
| Asia | 1047 | 1212 | 1447 | 1701 | 1949 | 2105 | 2445 | 2631 | 2851 | 2885 |
| Africa | 26 | 35 | 38 | 44 | 55 | 55 | 100 | 100 | 110 | 110 |
| Australia | 282 | 241 | 240 | 240 | 240 | 247 | 247 | 247 | 247 | 216 |
| Eurasia | 266 | 311 | 390 | 452 | 561 | 754 | 1006 | 1145 | 1200 | 1196 |
| Σ: | 15,858 | 16,587 | 17,464 | 18,490 | 19,314 | 20,160 | 20,417 | 20,835 | 21,122 | 21,283 |
| Substrate | Methane Yield | Advantages | Disadvantages |
|---|---|---|---|
| Maize silage | 294.5–376.2 m3 CH4/t vs. (Volatile Solids) [49] | High starch content (up to 35%) ensures rapid fermentation, has an optimal C:N ratio [50] | Low C:N ratio (may require nitrogen supplementation) and risk of soil degradation with intensive cultivation [51] |
| Grass silage | 341 m3 CH4/t vs. [52] | High availability and low production costs (marginal agricultural soils) [51] | Lower methane yield than maize (more lignin), greater structural requirements of the fermenter [51] |
| Cereal silage | 300.8 m3 CH4/t vs. for winter wheat, 277 m3 CH4/t vs. for oat [49] | Balanced composition (protein, carbohydrates); quantitatively available raw biomass; contains sugars and starch (e.g., wheat, triticale) that facilitate fermentation [53] | Conflict between food production and feedstock production for biogas plants [54] |
| Sorghum | 287.8 m3 CH4/t vs. [49], 277.25–301.86 m3 CH4/t vs. [33] | Drought-resistant, an alternative to maize in Europe, tolerates higher temperatures [33,55] | Lignin present in sorghum hinders microbial decomposition of the cell wall, which may negatively impact biogas production [56] |
| Oil | up to 1014 m3 CH4/t vs. [57] with 70% methane content in biogas | Huge energy potential—very high biochemical methane potential (BMP) [57] | Risk of inhibition by LCFAs (long-chain fatty acids) [58] |
| Whey | 395.6–565.8 m3 CH4/t vs. [59] | Very high bioavailability (high lactose content) [59] | Low alkalinity levels, tendency for rapid acidification [60] |
| Sugar beet pulp | open silos 337–420 m3 CH4/t VS, closed silos 411 to 451 m3 CH4/t vs. [61] | Sugar beet pulp contains 60–70% carbohydrates in dry matter, of which almost 90% is composed of cellulose, hemicellulose and pectin [62] | Seasonal availability from sugar refineries [63] |
| Raw brewer’s spent grains | 301 m3 CH4/t vs. [64], 387 m3 CH4/t vs. [65] | Rich in protein and readily available carbohydrates; a waste product [66] | Often requires pretreatment, partially difficult to decompose [67] |
| Food waste | 467–529 m3 CH4/t vs. [68] * | Very high biodegradability (high content of sugars, fats, proteins), gives a quick and high level of biogas [69] | High protein and nitrogen content, therefore, nitrogen-containing organic compounds, such as proteins, amino acids, and nucleic acids, can be hydrolyzed to ammonium nitrogen [70] |
| Fruit and vegetable waste | 420 m3 CH4/t vs. grape pomace, 231 m3 CH4/t vs. beet leaves [71] | Very easily biodegradable (lots of sugars, pectins), digests quickly [72] | Often high humidity, variable composition, rapid acidification, leading to decreased pH within the bioreactor [19,73] |
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Pochwatka, P. Evaluating the Performance of Biogas Plants Operating on Organic Waste: A Practical Approach. Energies 2026, 19, 2337. https://doi.org/10.3390/en19102337
Pochwatka P. Evaluating the Performance of Biogas Plants Operating on Organic Waste: A Practical Approach. Energies. 2026; 19(10):2337. https://doi.org/10.3390/en19102337
Chicago/Turabian StylePochwatka, Patrycja. 2026. "Evaluating the Performance of Biogas Plants Operating on Organic Waste: A Practical Approach" Energies 19, no. 10: 2337. https://doi.org/10.3390/en19102337
APA StylePochwatka, P. (2026). Evaluating the Performance of Biogas Plants Operating on Organic Waste: A Practical Approach. Energies, 19(10), 2337. https://doi.org/10.3390/en19102337

