Next Article in Journal
Walking Ability Outcome Measures in Individuals with Spinal Cord Injury: A Systematic Review
Next Article in Special Issue
Workers with Cardiac AIMD Exposed to EMF: Methods and Case Studies for Risk Analysis in the Framework of the European Regulations
Previous Article in Journal
Immediate Effects of Acupuncture on Explosive Force Production and Stiffness in Male Knee Joint
Previous Article in Special Issue
Radiofrequency Exposure Levels from Mobile Phone Base Stations in Outdoor Environments and an Underground Shopping Mall in Japan
Article

Anti-Oxidative and Immune Regulatory Responses of THP-1 and PBMC to Pulsed EMF Are Field-Strength Dependent

1
Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, 8010 Graz, Austria
2
Institute of Experimental Physics, Graz University of Technology, 8010 Graz, Austria
*
Author to whom correspondence should be addressed.
Academic Editors: Valentina Hartwig and Giuseppe Acri
Int. J. Environ. Res. Public Health 2021, 18(18), 9519; https://doi.org/10.3390/ijerph18189519
Received: 6 August 2021 / Revised: 7 September 2021 / Accepted: 8 September 2021 / Published: 9 September 2021
(This article belongs to the Special Issue Occupational and General Public Exposure to Electromagnetic Fields)
Innate immune cells react to electromagnetic fields (EMF) by generating reactive oxygen species (ROS), crucial intracellular messengers. Discrepancies in applied parameters of EMF studies, e.g., flux densities, complicate direct comparison of downstream anti-oxidative responses and immune regulatory signaling. We therefore compared the impact of different EMF flux densities in human leukemic THP1 cells and peripheral blood mononuclear cells (PBMC) of healthy donors to additionally consider a potential disparate receptivity based on medical origin. ROS levels increased in THP1 cells stimulated with lipopolysaccharide (LPS) after one hour of EMF exposure. Moreover, weak EMF mitigated the depletion of the reducing agent NAD(P)H in THP1. Neither of these effects occurred in PBMC. Landscaping transcriptional responses to varied EMF revealed elevation of the anti-oxidative enzymes PRDX6 (2-fold) and DHCR24 (6-fold) in THP1, implying involvement in lipid metabolism. Furthermore, our study confirmed anti-inflammatory effects of EMF by 6-fold increased expression of IL10. Strikingly, THP1 responded to weak EMF, while PBMC were primarily affected by strong EMF, yet with severe cellular stress and enhanced rates of apoptosis, indicated by HSP70 and caspase 3 (CASP3). Taken together, our results emphasize an altered susceptibility of immune cells of different origin and associate EMF-related effects with anti-inflammatory signaling and lipid metabolism. View Full-Text
Keywords: extremely low frequency pulsed electromagnetic fields (ELF-PEMF); reactive oxygen species (ROS); EM field strength; anti-oxidative response; immune stimulation extremely low frequency pulsed electromagnetic fields (ELF-PEMF); reactive oxygen species (ROS); EM field strength; anti-oxidative response; immune stimulation
Show Figures

Figure 1

MDPI and ACS Style

Groiss, S.; Lammegger, R.; Brislinger, D. Anti-Oxidative and Immune Regulatory Responses of THP-1 and PBMC to Pulsed EMF Are Field-Strength Dependent. Int. J. Environ. Res. Public Health 2021, 18, 9519. https://doi.org/10.3390/ijerph18189519

AMA Style

Groiss S, Lammegger R, Brislinger D. Anti-Oxidative and Immune Regulatory Responses of THP-1 and PBMC to Pulsed EMF Are Field-Strength Dependent. International Journal of Environmental Research and Public Health. 2021; 18(18):9519. https://doi.org/10.3390/ijerph18189519

Chicago/Turabian Style

Groiss, Silvia, Roland Lammegger, and Dagmar Brislinger. 2021. "Anti-Oxidative and Immune Regulatory Responses of THP-1 and PBMC to Pulsed EMF Are Field-Strength Dependent" International Journal of Environmental Research and Public Health 18, no. 18: 9519. https://doi.org/10.3390/ijerph18189519

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop