Next Article in Journal
Long-Term Neurological and Psychological Distress Symptoms among Smallholder Farmers in Costa Rica with a History of Acute Pesticide Poisoning
Next Article in Special Issue
Ethical Attitudes toward COVID-19 Passports: Evidences from Spain
Previous Article in Journal
Impact of Income, Density, and Population Size on PM2.5 Pollutions: A Scaling Analysis of 254 Large Cities in Six Developed Countries
Previous Article in Special Issue
COVID-19 Stressors on Migrant Workers in Vietnam: Cumulative Risk Consideration
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJERPH
Volume 18
Issue 17
10.3390/ijerph18179020
ijerph-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line

by
Luisa Zupin
1,*,
Francesco Fontana
2,
Rossella Gratton
1,
Margherita Milani
3,
Libera Clemente
2,
Lorella Pascolo
1,
Maurizio Ruscio
2 and
Sergio Crovella
4
1
Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy
2
Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASUGI), 34129 Trieste, Italy
3
Department of Medicine, Surgery and Health Sciences, University of Trieste, 34149 Trieste, Italy
4
Department of Biological and Environmental Sciences, College of Arts and Sciences, University of Qatar, Doha 2713, Qatar
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2021, 18(17), 9020; https://doi.org/10.3390/ijerph18179020
Submission received: 18 June 2021 / Revised: 18 August 2021 / Accepted: 25 August 2021 / Published: 26 August 2021
(This article belongs to the Special Issue SARS-CoV-2 Environmental Prevention Method: From Public Spaces to Medical Settings)
Browse Figures
Versions Notes

Abstract

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is mainly transmitted through respiratory droplets from positive subjects to susceptible hosts or by direct contact with an infected individual. Our study focuses on the in vitro minimal time of viral absorption as well as the minimal quantity of virus able to establish a persistent infection in Vero E6 cells. We observed that 1 min of in vitro virus exposure is sufficient to generate a cytopathic effect in cells after 7 days of infection, even at a multiplicity of infection (MOI) value of 0.01. Being aware that our findings have been obtained using an in vitro cellular model, we demonstrated that short-time exposures and low viral concentrations are able to cause infection, thus opening questions about the risk of SARS-CoV-2 transmissibility even following short contact times.
Keywords: SARS-CoV-2; infection; Vero E6 cells SARS-CoV-2; infection; Vero E6 cells

Share and Cite

MDPI and ACS Style

Zupin, L.; Fontana, F.; Gratton, R.; Milani, M.; Clemente, L.; Pascolo, L.; Ruscio, M.; Crovella, S. SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line. Int. J. Environ. Res. Public Health 2021, 18, 9020. https://doi.org/10.3390/ijerph18179020

AMA Style

Zupin L, Fontana F, Gratton R, Milani M, Clemente L, Pascolo L, Ruscio M, Crovella S. SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line. International Journal of Environmental Research and Public Health. 2021; 18(17):9020. https://doi.org/10.3390/ijerph18179020

Chicago/Turabian Style

Zupin, Luisa, Francesco Fontana, Rossella Gratton, Margherita Milani, Libera Clemente, Lorella Pascolo, Maurizio Ruscio, and Sergio Crovella. 2021. "SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line" International Journal of Environmental Research and Public Health 18, no. 17: 9020. https://doi.org/10.3390/ijerph18179020

APA Style

Zupin, L., Fontana, F., Gratton, R., Milani, M., Clemente, L., Pascolo, L., Ruscio, M., & Crovella, S. (2021). SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line. International Journal of Environmental Research and Public Health, 18(17), 9020. https://doi.org/10.3390/ijerph18179020

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop