Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Media
2.2. Instrumental Analysis
2.3. Enrichment and Isolation of DEHP-Degrading Strain
2.4. Molecular Identification of DEHP-Degrading Strain
2.5. Inoculum Preparation
2.6. Effects of Environmental Factors on DEHP Degradation
2.7. Degradation Efficiency of DEHP at Maximum and Minimum Concentrations
2.8. Substrate Utilization Tests
2.9. Analysis of Intermediates
2.10. Decontamination of Artificially DEHP-Polluted Soil
2.11. Statistical Analysis
3. Results
3.1. Microbial Isolation and Identification of DEHP-Degrading Bacterium
3.2. Utilization of DEHP as Sole Source of Carbon for Growth by YC-IL1
3.3. Effects of Environmental Factors on DEHP Degradation
3.3.1. Effect of Temperature on DEHP Degradation by YC-IL1
3.3.2. Effect of pH on DEHP Degradation by YC-IL1
3.3.3. Effect of NaCl Concentration on DEHP Degradation by YC-IL1
3.4. Substrate Spectrum of YC-IL1
3.5. Degradation Efficiency of DEHP at Maximum and Minimum Concentrations
3.6. Deduction of DEHP Degradation Pathway
3.7. Bioremediation of DEHP-Contaminated Soil
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chemicals | Formula | MW (g/mol) | Retention Time (min) | m/z | ESI Polarity |
---|---|---|---|---|---|
DEHP (Substrate) | C24H38O4 | 390.56 | 7.369 | 149.0&391.1&413.1&436.2 | Positive |
MEHP | C16H22O4 | 278.34 | 2.607 | 277.0 | Negative |
PA | C8H6O4 | 166.13 | 1.443 | 164.9&121.0 | Negative. |
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Lamraoui, I.; Eltoukhy, A.; Wang, J.; Lamraoui, M.; Ahmed, A.; Jia, Y.; Lu, T.; Yan, Y. Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria. Int. J. Environ. Res. Public Health 2020, 17, 7501. https://doi.org/10.3390/ijerph17207501
Lamraoui I, Eltoukhy A, Wang J, Lamraoui M, Ahmed A, Jia Y, Lu T, Yan Y. Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria. International Journal of Environmental Research and Public Health. 2020; 17(20):7501. https://doi.org/10.3390/ijerph17207501
Chicago/Turabian StyleLamraoui, Imane, Adel Eltoukhy, Junhuan Wang, Messaouda Lamraoui, Amer Ahmed, Yang Jia, Tiegang Lu, and Yanchun Yan. 2020. "Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria" International Journal of Environmental Research and Public Health 17, no. 20: 7501. https://doi.org/10.3390/ijerph17207501