Streptinone, a New Indanone Derivative from a Marine-Derived Streptomyces massiliensis, Inhibits Particulate Matter-Induced Inflammation
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. General Experimental Procedures and Reagents
3.2. Strain and Fermentation
3.3. Extraction and Isolation of Compounds
3.4. Computational Analysis
3.5. Cell Culture and Cell Viability Evaluation
3.6. Cytoprotectivity and NO-Inhibitory Activity Evaluation
3.7. Evaluation of PGE2 and Levels of Pro-Inflammatory Cytokines in PM-Stimulated RAW 264.7 Cells
3.8. Gene Expression Analysis
3.9. Protein Expression Evaluation
3.10. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No | δC, Type | δH, Mult. (J in Hz) |
---|---|---|
1 | 209.7, C | |
2 | 36.9, CH2 | 2.52 t (overlapped) |
3 | 27.6, CH2 | 2.93 m 2.66 m |
4 | 180.0, C | |
5 | 136.2, C | |
6 | 132.5, C | |
7 | 131.2, CH | 5.70 br s |
8 | 39.3, CH | 2.54 m (overlapped) |
9 | 75.0, CH | 4.26 d (12.2) |
10 | 62.9, CH2 | 4.33 d (13.6) 4.29 d (13.6) |
11 | 18.4, CH3 | 1.20 d (7.2) |
No | Primer Name | Sequence |
---|---|---|
1 | GAPDH forward | 5′-AAGGGTCATCATCTCTGCCC-3′ |
2 | GAPDH reverse | 5′-GTGATGGCATGGACTGTGGT-3′ |
3 | TNF-α forward | 5′-TTGACCTCAGCGCTGAGTTG-3′ |
4 | TNF-α reverse | 5′-CCTGTAGCCCACGTCGTAGC-3′ |
5 | IL-6 forward | 5′-GTACTCCAGAAGACCAGAGG-3′ |
6 | IL-6 reverse | 5′-TGCTGGTGACAACCACGGCC-3′ |
7 | IL-1β forward | 5′-CAGGATGAGGACATGAGCACC-3′ |
8 | IL-1β reverse | 5′-CTCTGCAGACTCAAACTCCAC-3′ |
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Lee, H.-S.; Nagahawatta, D.P.; Jeon, Y.-J.; Lee, M.A.; Heo, C.-S.; Park, S.J.; Shin, H.J. Streptinone, a New Indanone Derivative from a Marine-Derived Streptomyces massiliensis, Inhibits Particulate Matter-Induced Inflammation. Mar. Drugs 2023, 21, 640. https://doi.org/10.3390/md21120640
Lee H-S, Nagahawatta DP, Jeon Y-J, Lee MA, Heo C-S, Park SJ, Shin HJ. Streptinone, a New Indanone Derivative from a Marine-Derived Streptomyces massiliensis, Inhibits Particulate Matter-Induced Inflammation. Marine Drugs. 2023; 21(12):640. https://doi.org/10.3390/md21120640
Chicago/Turabian StyleLee, Hwa-Sun, Dineth Pramuditha Nagahawatta, You-Jin Jeon, Min Ah Lee, Chang-Su Heo, Sun Joo Park, and Hee Jae Shin. 2023. "Streptinone, a New Indanone Derivative from a Marine-Derived Streptomyces massiliensis, Inhibits Particulate Matter-Induced Inflammation" Marine Drugs 21, no. 12: 640. https://doi.org/10.3390/md21120640