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Article

A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans

1
A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia
2
Institute of Genetics and Cancer, The University of Edinburgh, Edinburgh EH4 2XU, UK
3
Institute of Life Sciences and Biomedicine, Far Eastern Federal University, 690922 Vladivostok, Russia
*
Author to whom correspondence should be addressed.
Academic Editors: Yasuhiro Ozeki, Yuki Fujii and Marco Gerdol
Mar. Drugs 2021, 19(12), 668; https://doi.org/10.3390/md19120668
Received: 28 October 2021 / Revised: 18 November 2021 / Accepted: 24 November 2021 / Published: 26 November 2021
(This article belongs to the Special Issue Marine Glycomics)
C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) in a lectin-like manner. A new protein MkC1qDC from the hemolymph plasma of Modiolus kurilensis bivalve mollusk widespread in the Northwest Pacific was purified. The isolation procedure included ammonium sulfate precipitation followed by affinity chromatography on pectin-Sepharose. The full-length MkC1qDC sequence was assembled using de novo mass-spectrometry peptide sequencing complemented with N-terminal Edman’s degradation, and included 176 amino acid residues with molecular mass of 19 kDa displaying high homology to bivalve C1qDC proteins. MkC1qDC demonstrated antibacterial properties against Gram-negative and Gram-positive strains. MkC1qDC binds to a number of saccharides in Ca2+-dependent manner which characterized by structural meta-similarity in acidic group enrichment of galactose and mannose derivatives incorporated in diversified molecular species of glycans. Alginate, κ-carrageenan, fucoidan, and pectin were found to be highly effective inhibitors of MkC1qDC activity. Yeast mannan, lipopolysaccharide (LPS), peptidoglycan (PGN) and mucin showed an inhibitory effect at concentrations three orders of magnitude greater than for the most effective saccharides. MkC1qDC localized to the mussel hemal system and interstitial compartment. Intriguingly, MkC1qDC was found to suppress proliferation of human adenocarcinoma HeLa cells in a dose-dependent manner, indicating to the biomedical potential of MkC1qDC protein. View Full-Text
Keywords: bivalve mollusk; C1q domain-containing; lectin-like; pattern recognition receptor; polysaccharides; interstitial compartment bivalve mollusk; C1q domain-containing; lectin-like; pattern recognition receptor; polysaccharides; interstitial compartment
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MDPI and ACS Style

Grinchenko, A.V.; von Kriegsheim, A.; Shved, N.A.; Egorova, A.E.; Ilyaskina, D.V.; Karp, T.D.; Goncharov, N.V.; Petrova, I.Y.; Kumeiko, V.V. A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans. Mar. Drugs 2021, 19, 668. https://doi.org/10.3390/md19120668

AMA Style

Grinchenko AV, von Kriegsheim A, Shved NA, Egorova AE, Ilyaskina DV, Karp TD, Goncharov NV, Petrova IY, Kumeiko VV. A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans. Marine Drugs. 2021; 19(12):668. https://doi.org/10.3390/md19120668

Chicago/Turabian Style

Grinchenko, Andrei V., Alex von Kriegsheim, Nikita A. Shved, Anna E. Egorova, Diana V. Ilyaskina, Tatiana D. Karp, Nikolay V. Goncharov, Irina Y. Petrova, and Vadim V. Kumeiko. 2021. "A Novel C1q Domain-Containing Protein Isolated from the Mollusk Modiolus kurilensis Recognizing Glycans Enriched with Acidic Galactans and Mannans" Marine Drugs 19, no. 12: 668. https://doi.org/10.3390/md19120668

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