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Open AccessArticle

Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata

by ZhenXing Du 1,2,3, XueJing Jia 1,3, Jing Chen 1,2,3, SiYi Zhou 1,2,3, JianPing Chen 1, XiaoFei Liu 1, XiaoHuang Cao 1, SaiYi Zhong 1,2,3,4,* and PengZhi Hong 1,2,3
1
School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
2
Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China
3
Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China
4
Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(1), 6; https://doi.org/10.3390/md18010006
Received: 19 October 2019 / Revised: 23 November 2019 / Accepted: 3 December 2019 / Published: 19 December 2019
(This article belongs to the Special Issue Marine Molecules for the Treatment of Thrombosis)
Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong anti-thrombotic activities. This study reports on a purified heparinoid from Coelomactra antiquata, which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S (or GlcNS6S)-(1→4)-β-GlcA-(1→4)-α-GlcNS6S (or GlcNAC)-(1→. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU/mg and 1.96 IU/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry. View Full-Text
Keywords: shellfish; heparin; structural characteristics; anticoagulant activity; fibrinolytic activity shellfish; heparin; structural characteristics; anticoagulant activity; fibrinolytic activity
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Du, Z.; Jia, X.; Chen, J.; Zhou, S.; Chen, J.; Liu, X.; Cao, X.; Zhong, S.; Hong, P. Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata. Mar. Drugs 2020, 18, 6.

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