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Functional Characterization of Carbohydrate-Binding Modules in a New Alginate Lyase, TsAly7B, from Thalassomonas sp. LD5
Open AccessArticle

Elucidation of a Unique Pattern and the Role of Carbohydrate Binding Module of an Alginate Lyase

1
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China
2
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, CAS, Dalian 116023, China
3
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(1), 32; https://doi.org/10.3390/md18010032
Received: 3 November 2019 / Revised: 12 December 2019 / Accepted: 13 December 2019 / Published: 30 December 2019
Alginate oligosaccharides with different degrees of polymerization (DPs) possess diverse physiological activities. Therefore, in recent years, increasing attention has been drawn to the use of enzymes for the preparation of alginate oligosaccharides for food and industrial applications. Previously, we identified and characterized a novel bifunctional alginate lyase Aly7A, which can specifically release trisaccharide from three different substrate types with a unique degradation pattern. Herein, we investigated its degradation pattern by modular truncation and molecular docking. The results suggested that Aly7A adopted a unique action mode towards different substrates with the substrate chain sliding into the binding pocket of the catalytic domain to position the next trisaccharide for cleavage. Deletion of the Aly7A carbohydrate binding module (CBM) domain resulted in a complex distribution of degradation products and no preference for trisaccharide formation, indicating that the CBM may act as a “controller” during the trisaccharide release process. This study further testifies CBM as a regulator of product distribution and provides new insights into well-defined generation of alginate oligosaccharides with associated CBMs. View Full-Text
Keywords: alginate lyase; trisaccharide preparation; degrading pattern; unique mechanism; oligosaccharides alginate lyase; trisaccharide preparation; degrading pattern; unique mechanism; oligosaccharides
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MDPI and ACS Style

Hu, F.; Zhu, B.; Li, Q.; Yin, H.; Sun, Y.; Yao, Z.; Ming, D. Elucidation of a Unique Pattern and the Role of Carbohydrate Binding Module of an Alginate Lyase. Mar. Drugs 2020, 18, 32.

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