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Open AccessArticle

Characterization of the Jumbo Squid (Dosidicus gigas) Skin By-Product by Shotgun Proteomics and Protein-Based Bioinformatics

1
Department of Food Technology, Marine Research Institute (IIM), Spanish National Research Council (CSIC), 36208 Vigo, Pontevedra, Spain
2
Department of Food Research and Postgraduate, University of Sonora, 83100 Hermosillo, Sonora, Mexico
*
Author to whom correspondence should be addressed.
Mar. Drugs 2020, 18(1), 31; https://doi.org/10.3390/md18010031
Received: 26 November 2019 / Revised: 19 December 2019 / Accepted: 26 December 2019 / Published: 29 December 2019
Jumbo squid (Dosidicus gigas) is one of the largest cephalopods, and represents an important economic fishery in several regions of the Pacific Ocean, from southern California in the United States to southern Chile. Large and considerable discards of this species, such as skin, have been reported to constitute an important source of potential by-products. In this paper, a shotgun proteomics approach was applied for the first time to the characterization of the jumbo squid (Dosidicus gigas) skin proteome. A total of 1004 different peptides belonging to 219 different proteins were identified. The final proteome compilation was investigated by integrated in-silico studies, including gene ontology (GO) term enrichment, pathways, and networks studies. Potential new valuable bioactive peptides such as antimicrobial, bioactive collagen peptides, antihypertensive and antitumoral peptides were predicted to be present in the jumbo squid skin proteome. The integration of the global proteomics results and the bioinformatics analysis of the jumbo squid skin proteome show a comprehensive knowledge of this fishery discard and provide potential bioactive peptides of this marine by-product. View Full-Text
Keywords: Dosidicus gigas; squid; skin; by-product; shotgun proteomics; mass spectrometry; protein-based bioinformatics; bioactive peptides Dosidicus gigas; squid; skin; by-product; shotgun proteomics; mass spectrometry; protein-based bioinformatics; bioactive peptides
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Carrera, M.; Ezquerra-Brauer, J.M.; Aubourg, S.P. Characterization of the Jumbo Squid (Dosidicus gigas) Skin By-Product by Shotgun Proteomics and Protein-Based Bioinformatics. Mar. Drugs 2020, 18, 31.

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