Next Article in Journal
Identification of Quorum Sensing Activators and Inhibitors in The Marine Sponge Sarcotragus spinosulus
Next Article in Special Issue
Two New Alginate Lyases of PL7 and PL6 Families from Polysaccharide-Degrading Bacterium Formosa algae KMM 3553T: Structure, Properties, and Products Analysis
Previous Article in Journal
Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems
Previous Article in Special Issue
Elucidation of a Unique Pattern and the Role of Carbohydrate Binding Module of an Alginate Lyase
Open AccessArticle

Characterization of a New Chitosanase from a Marine Bacillus sp. and the Anti-Oxidant Activity of Its Hydrolysate

College of Basic Medicine, Qingdao University, Qingdao 266071, China
*
Author to whom correspondence should be addressed.
These authors contributed equally for this paper.
Mar. Drugs 2020, 18(2), 126; https://doi.org/10.3390/md18020126
Received: 22 January 2020 / Revised: 14 February 2020 / Accepted: 14 February 2020 / Published: 19 February 2020
Chitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene, csnQ, was cloned from the marine Bacillus sp. Q1098 and expressed in Escherichia coli. The recombinant chitosanase, CsnQ, showed maximal activity at pH 5.31 and 60 °C. Determination of CsnQ pH-stability showed that CsnQ could retain more than 50% of its activity over a wide pH, from 3.60 to 9.80. CsnQ is an endo-type chitosanase, yielding chitodisaccharide as the main product. Additionally, in vitro and in vivo analyses indicated that chitodisaccharide possesses much more effective anti-oxidant activity than glucosamine and low molecular weight chitosan (LMW-CS) (~5 kDa). Notably, to our knowledge, this is the first evidence that chitodisaccharide is the minimal COS fragment required for free radical scavenging. View Full-Text
Keywords: chitosanase; pH-stability; Bacillus sp. Q1098; chitooligosaccharide; anti-oxidant activity chitosanase; pH-stability; Bacillus sp. Q1098; chitooligosaccharide; anti-oxidant activity
Show Figures

Figure 1

MDPI and ACS Style

Ma, C.; Li, X.; Yang, K.; Li, S. Characterization of a New Chitosanase from a Marine Bacillus sp. and the Anti-Oxidant Activity of Its Hydrolysate. Mar. Drugs 2020, 18, 126.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop