A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa
Abstract
:1. Introduction
2. Results
2.1. Isolation and Taxonomical Identification of QSI-producing Bacteria from Anemones and Holothurians
2.2. Effect of Cell Pellet Extract from Strain M3-10 on C. Violaceum ATCC 12472 Violacein Production, Pyoverdine Production, Biofilm Formation, and Pseudomonas aeruginosa PAO1 Motility
2.3. Identification of QSI Compounds from V. alginolyticus M3-10
2.4. Evaluation of QSI Activity of Tyramine and N-acetyltyramine Standards
2.5. Whole-Genome Analysis of V. alginolyticus M3-10
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Media, Compounds, and Culture Conditions
4.2. Screening for QS Inhibition Activity
4.3. Preparation of Methanolic Extracts of QSI-producing Bacteria
4.4. Violacein Production Assay
4.5. Pseudomonas Virulence Factor Tests: Pyoverdine, Biofilm, and Motility
4.6. Bioassay-Guided Fractionation and Identification of QSI Compounds by LC–DAD–HRMS and NMR
4.7. QSI inhibition Activity Test of Tyramine and N-acetyltyramine
4.8. Taxonomic Identification and Genome Analysis of Strain M3-10
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Strain | Identification | Violacein Inhibition Halo (mm) |
---|---|---|
M1-9 | Vibrio neocaledonicus | 16 |
M2-99 | Vibrio antiquarius | 13 |
M3-10 | Vibrio alginolyticus | 18 |
M3-21 | V. neocaledonicus | 15 |
M3-78 | V. alginolyticus | 15 |
M3-99 | V. alginolyticus | 15 |
M4-31 | V. alginolyticus | 17 |
M4-35 | V. alginolyticus | 12 |
M4-116 | V. alginolyticus | 13 |
M4-117 | V. neocaledonicus | 12 |
M4-119 | V. neocaledonicus | 16 |
M4-126 | V. neocaledonicus | 11 |
M5-23 | V. neocaledonicus | 13 |
M5-35 | V. neocaledonicus | 16 |
M5-47 | V. neocaledonicus | 18 |
M5-50 | V. neocaledonicus | 13 |
M6-17 | V. neocaledonicus | 13 |
M6-26 | V. neocaledonicus | 13 |
M6-31 | V. alginolyticus | 11 |
M6-39 | V. zhuhaiensis | 14 |
M6-50 | V. neocaledonicus | 17 |
M6-66 | V. alginolyticus | 15 |
M10-18 | V. neocaledonicus | 14 |
Extract | Violacein Production (Absorbance at 585 nm) | |
---|---|---|
M3-10 | cell pellet | 0.034 |
supernatant | 0.206 | |
M4-31 | cell pellet | 0.044 |
supernatant | 0.282 | |
M4-119 | cell pellet | 0.06 |
supernatant | 0.276 | |
M5-47 | cell pellet | 0.044 |
supernatant | 0.216 | |
M6-50 | cell pellet | 0.042 |
supernatant | 0.280 | |
MeOH (negative control) | 0.489 |
Mean in the Absence Of Cell Pellet Extract | Mean in the Presence Of Cell Pellet Extract | |
---|---|---|
C. violaceum ATCC 12472 | ||
Violacein (% production) | 100 [±10.9] | 33.89 * [±17.1] |
P. aeruginosa PAO1 | ||
Pyoverdine (% production) | 100 [±16.1] | 74.02 * [±8] |
Biofilm (% production) | 100 [±12.7] | 71.96 * [±5.9] |
Swimming (% motility) | 55.6 [±8.1] | 42.8 * [±2.5] |
Swarming (% motility) | 39.46 [±15.3] | 27.36 [±9.6] |
Twitching (% motility) | 39.15 [±13.6] | 31.54 [±10.7] |
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Reina, J.C.; Pérez-Victoria, I.; Martín, J.; Llamas, I. A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa. Mar. Drugs 2019, 17, 494. https://doi.org/10.3390/md17090494
Reina JC, Pérez-Victoria I, Martín J, Llamas I. A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa. Marine Drugs. 2019; 17(9):494. https://doi.org/10.3390/md17090494
Chicago/Turabian StyleReina, José Carlos, Ignacio Pérez-Victoria, Jesús Martín, and Inmaculada Llamas. 2019. "A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa" Marine Drugs 17, no. 9: 494. https://doi.org/10.3390/md17090494