Bioactive Brominated Oxindole Alkaloids from the Red Sea Sponge Callyspongia siphonella
AbstractIn the present study, LC-HRESIMS-assisted dereplication along with bioactivity-guided isolation led to targeting two brominated oxindole alkaloids (compounds 1 and 2) which probably play a key role in the previously reported antibacterial, antibiofilm, and cytotoxicity of Callyspongia siphonella crude extracts. Both metabolites showed potent antibacterial activity against Gram-positive bacteria, Staphylococcus aureus (minimum inhibitory concentration (MIC) = 8 and 4 µg/mL) and Bacillus subtilis (MIC = 16 and 4 µg/mL), respectively. Furthermore, they displayed moderate biofilm inhibitory activity in Pseudomonas aeruginosa (49.32% and 41.76% inhibition, respectively), and moderate in vitro antitrypanosomal activity (13.47 and 10.27 µM, respectively). In addition, they revealed a strong cytotoxic effect toward different human cancer cell lines, supposedly through induction of necrosis. This study sheds light on the possible role of these metabolites (compounds 1 and 2) in keeping fouling organisms away from the sponge outer surface, and the possible applications of these defensive molecules in the development of new anti-infective agents. View Full-Text
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El-Hawary, S.S.; Sayed, A.M.; Mohammed, R.; Hassan, H.M.; Rateb, M.E.; Amin, E.; Mohammed, T.A.; El-Mesery, M.; Bin Muhsinah, A.; Alsayari, A.; Wajant, H.; Anany, M.A.; Abdelmohsen, U.R. Bioactive Brominated Oxindole Alkaloids from the Red Sea Sponge Callyspongia siphonella. Mar. Drugs 2019, 17, 465.
El-Hawary SS, Sayed AM, Mohammed R, Hassan HM, Rateb ME, Amin E, Mohammed TA, El-Mesery M, Bin Muhsinah A, Alsayari A, Wajant H, Anany MA, Abdelmohsen UR. Bioactive Brominated Oxindole Alkaloids from the Red Sea Sponge Callyspongia siphonella. Marine Drugs. 2019; 17(8):465.Chicago/Turabian Style
El-Hawary, Seham S.; Sayed, Ahmed M.; Mohammed, Rabab; Hassan, Hossam M.; Rateb, Mostafa E.; Amin, Elham; Mohammed, Tarek A.; El-Mesery, Mohamed; Bin Muhsinah, Abdullatif; Alsayari, Abdulrhman; Wajant, Harald; Anany, Mohamed A.; Abdelmohsen, Usama R. 2019. "Bioactive Brominated Oxindole Alkaloids from the Red Sea Sponge Callyspongia siphonella." Mar. Drugs 17, no. 8: 465.
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